Abstract：
:Although red blood cell (RBC) antigen typing by agglutination is generally useful, several situations exist where this approach is difficult or impossible. For example, following a massive transfusion, a patient's residual RBCs are mixed with transfused normal donor RBCs. In this case, typing by hemagglutination primarily detects the antigens on the heterogeneous population of transfused RBCs. Agglutination testing is also of limited use for determining the phenotype of a fetus at risk for hemolytic disease of the newborn because fetal RBCs must be obtained by periumbilical blood sampling. Determining the genotype of an individual by analyzing genomic DNA isolated from peripheral blood nucleated cells or amniocytes is an alternative approach for determining the RBC antigen type. In this report, the allele specific polymerase chain reaction (AS-PCR) was used to identify the alleles at the MN and Ss loci that encode the corresponding antigens on glycophorin A (GPA) and glycophorin B (GPB), respectively. This method was used to type these alleles in peripheral blood samples obtained from normal individuals and from patients following massive transfusion. Of 23 peripheral blood specimens analyzed, all were correctly typed by this method. The allele specific polymerase chain reaction was also used to determine these alleles using amniotic fluid samples. Of 11 amniotic fluid specimens analyzed, 8 were correctly typed at both loci. Mistyping of three amniotic fluid specimens was explained by possible maternal blood contamination.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Eshleman JR,Shakin-Eshleman SH,Church A,Kant JA,Spitalnik SLdoi
10.1093/ajcp/103.3.353subject
Has Abstractpub_date
1995-03-01 00:00:00pages
353-7issue
3eissn
0002-9173issn
1943-7722journal_volume
103pub_type
杂志文章abstract：:Cytology frequently has some role in preoperatively distinguishing pancreatic mucus-producing neoplasia (intraductal papillary mucinous neoplasms [IPMNs] and mucinous cystic neoplasms [MCNs]) from other pancreatic cysts. We evaluated all cytologic specimens at our institutions from resected pancreatic cystic lesions f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/Y16QCXE3YGMXX19Q
更新日期：2008-01-01 00:00:00
abstract：:The authors report a case of metastatic prostate cancer masquerading as a primary lung tumor. Histologically, the lung tumor displayed eosinophilic crystalloids in the malignant glands typical of those previously described in prostatic adenocarcinoma. Review of histologic material from 30 additional patients with meta...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.3.266
更新日期：1994-03-01 00:00:00
abstract：:Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.1.89
更新日期：1995-07-01 00:00:00
abstract：:In view of their reported reciprocal effects on apoptosis, the expression of p53 and bcl-2 proteins was studied in 46 cases of Hodgkin's disease by immunocytochemical labeling. We found p53 protein in Reed-Sternberg cells and their mononuclear variants in 16 of the 46 cases (34.7%) of Hodgkin's disease, mainly in a nu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.6.663
更新日期：1993-06-01 00:00:00
abstract：:A case of septicemia caused by Pseudomonas paucimobilis is reported. This represents the first definitive case of disease produced by this yellow-pigmented, nonfermentative bacillus. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.5.882
更新日期：1979-11-01 00:00:00
abstract：:Critical Path Method (CPM) and Program Evaluation and Review Technique (PERT) are quantitative network planning strategies that facilitate management of projects with many interrelated activities. They determine the minimum time necessary to complete a project and identify which activities are critical to achieving th...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.3.353
更新日期：1983-03-01 00:00:00
abstract：:This study compared the ability to diagnose and accurately classify hepatic mass lesions by ultrasound-guided biopsy using two different needle types: a 20 gauge needle that yielded primarily cytologic material and an 18 gauge automated core biopsy needle. Slides prepared from each of the needle types were separately ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.5.583
更新日期：1995-11-01 00:00:00
abstract：:We describe 13 cases in which the submucosa and muscularis mucosae of the gastrointestinal tract exhibited a focal or diffuse increase of elastin fibers. This elastosis or elastofibromatous change most commonly manifested as a colonic polyp and usually was found during screening colonoscopy. Gastric and small intestin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/LBGT-CG1Q-CD96-M3NF
更新日期：2004-08-01 00:00:00
abstract：:The cases of 20 patients, each of whom has a positive lupus erythematosus cell preparation and a negative antinuclear factor test, are presented. The concept of a false-positive lupus erythematosus preparation is suggested. Five common mechanisms causing a false-negative antinuclear factor test are discussed and evalu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.3.495
更新日期：1976-09-01 00:00:00
abstract：:Fourteen patients treated by allogeneic bone marrow transplantation for chronic myelogenous leukemia (CML) were evaluated by the polymerase chain reaction (PCR) for bcr-abl-specific transcripts. Nine patients were transplanted in the first chronic phase, three in the second chronic phase, and two in the accelerated ph...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.5.510
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVES:To compare next-generation sequencing (NGS) platforms with mutation-specific analysis platforms in a clinical setting, in terms of sensitivity, mutation specificity, costs, capacity, and ease of use. METHODS:We analyzed 25 formalin-fixed, paraffin-embedded lung cancer samples of different size and tumor per...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP40XETVYAMJPY
更新日期：2015-04-01 00:00:00
abstract：OBJECTIVES:We report a case of a patient with diabetes mellitus and unexpectedly low hemoglobin A1c results associated with paroxysmal nocturnal hemoglobinuria (PNH). We review the impact of shortened RBC half-life on the interpretation of hemoglobin A1c levels. METHODS:Patient history and laboratory test results were...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPTK1HPR2KULJU
更新日期：2014-08-01 00:00:00
abstract：OBJECTIVES:An intraoperative consultation (IOC) checklist was developed and implemented aimed at standardizing slide labeling and monitoring metrics central to quality and safety in surgical pathology. DESIGN:Data were collected for all IOC cases over a 9-month period. Slide labeling defect rates and IOC turnaround ti...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz009
更新日期：2019-05-03 00:00:00
abstract：OBJECTIVES:This session of the 2013 Society of Hematopathology/European Association for Haematopathology Workshop was dedicated to B-acute lymphoblastic leukemia (B-ALL)/lymphoblastic lymphoma (LBL) with recurrent translocations and not otherwise specified. METHODS:In this review, we summarize the cases discussed duri...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPAN7BH5DNYWZB
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVES:Although diffuse alveolar damage, a subtype of acute lung injury (ALI), is the most common microscopic pattern in coronavirus disease 2019 (COVID-19), other pathologic patterns have been described. The aim of the study was to review autopsies from COVID-19 decedents to evaluate the spectrum of pathology and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa156
更新日期：2020-11-04 00:00:00
abstract：:The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sens...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPI72YAXRHYHEE
更新日期：2010-04-01 00:00:00
abstract：OBJECTIVES:The coronavirus disease 2019 pandemic has halted in-person medical student education in many large academic centers, including the University of Washington. We identified a unique opportunity to bring comprehensive and targeted anatomic pathology training to large numbers of medical students who would not re...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa154
更新日期：2020-10-13 00:00:00
abstract：:Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPVFY95MAOBKRS
更新日期：2011-02-01 00:00:00
abstract：:Patients using anticoagulation point-of-care (POC) monitors are advised to periodically test these systems against laboratory methods to monitor performance. The international normalized ratio (INR), however, can vary between test systems owing to different instrument-reagent combinations. In a randomized study evalua...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2005-02-01 00:00:00
abstract：:Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.1.35
更新日期：1991-01-01 00:00:00
abstract：:A three-and-a-half-year old boy suffering from streptococcal pneumonia developed hemolytic-uremic syndrome and disseminated intravascular coagulation (DIC). His red blood cells (RBC) were shown to be T- and Tk-activated; serial testing showed his mature RBCs as well as neocytes remained T-activated at 40 days. Anti-T ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.2.244
更新日期：1985-08-01 00:00:00
abstract：:Two separate courses in clinical pathology were presented to two groups of second-year medical students. The first (large) group was taught by the conventional lecture method; the other (small) group, by a self-instructional method for roughly half of the course, followed by the conventional lecture method for the sec...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.6.847
更新日期：1978-12-01 00:00:00
abstract：:We tested the hypothesis that extensively keratinized squamous intraepithelial lesions (SILs) are difficult to grade precisely by identifying 100 Papanicolaou smears with a keratinizing SIL that had been originally judged difficult to grade. Of these, 65 were confirmed as low-grade SIL (LSIL) or high-grade SIL (HSIL) ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/RQBN-Y51J-CJTK-P9H5
更新日期：2001-01-01 00:00:00
abstract：:Campylobacter fetus subspecies jejuni has recently been recognized as a significant cause of enteritis in humans. The organism has been previously postulated to cause illness by direct invasion of the mucosa of the gastrointestinal tract, but this has not been documented. Reported is a case of Campylobacter enteritis ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.5.706
更新日期：1980-05-01 00:00:00
abstract：:The Digene Hybrid Capture 2 (hc2) high-risk human papillomavirus (HPV) DNA test (Digene, Gaithersburg, MD) is widely used for triage of women with atypical squamous cells of undetermined significance. Results in a "retest zone" (weakly positive tests) are repeated up to 2 times according to the Digene-recommended algo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/4WCPTUV506HLP06P
更新日期：2007-01-01 00:00:00
abstract：:Therapeutic control achieved by an anticoagulant clinic may be assessed by calculation of the proportion of time spent by the patients within the prothrombin time target range aimed for by the clinic. Three calculation methods for such assessment have been described by other investigators. The three methods were compa...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1093/ajcp/90.6.685
更新日期：1988-12-01 00:00:00
abstract：:Counterimmunoelectrophoresis was used to detect Cryptococcus neoformans capsular polysaccharide in the body fluids of infected patients. Using rabbit anti-C. neoformans A15 globulin, counterimmunoelectrophoresis detected 1.25 microgram of purified cryptococcal polysaccharide per ml of cerebrospinal fluid (CSF) or urin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.1.39
更新日期：1977-07-01 00:00:00
abstract：:The clinical, light microscopic, and immunohistochemical features of 14 sinonasal malignant melanomas were studied to show their diverse morphologic appearance and distinction from therapeutically more amenable neoplasms that occur in this region. The tumors arose in 6 men and 8 women (median age, 70 years). Eleven pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.6.689
更新日期：1991-12-01 00:00:00
abstract：:The effects of physiologic temperature variations on antibiotic-induced killing of Escherichia coli, Pseudomonas aeruginosa, Streptococcus faecalis, and Streptococcus pneumoniae were studied. At concentrations less than or equal to four times the minimal bactericidal concentration, the activity of each of the antibiot...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.1.57
更新日期：1981-07-01 00:00:00
abstract：:Data collection can be enhanced with the use of computers. Care must be taken, however, to ensure that the data collected have a purpose and meet either the quality assessment needs or business functions of the transfusion service. Required data elements are frequently in several data repositories and must be merged t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1997-04-01 00:00:00
