Quantitative measurement of HbA1c by an immunoturbidimetric assay compared to a standard HPLC method.

Abstract:

:Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatography for total GHb. In this study, a new turbidimetric immunoassay for HbA1c (Boehringer Mannheim, Germany) was evaluated that was performed on a Hitachi 911 clinical chemistry analyzer (Boehringer Mannheim). Good linearity in the range of 5% to 15% HbA1c, within-run and between-run coefficients of variation ranging from 2.4% to 5.9% were obtained. Results of 179 diabetic and nondiabetic patients showed good correlation to those of a routine HPLC method (r = 0.96). In addition, HbA2, HbS, and HbF in samples from nondiabetic patients were not detected by the immunoturbimetric assay and the "labile" HbA1c fraction (Schiff base) did not interfere with the new test.

journal_name

Am J Clin Pathol

authors

Hamwi A,Schweiger CR,Veitl M,Schmid R

doi

10.1093/ajcp/104.1.89

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

89-95

issue

1

eissn

0002-9173

issn

1943-7722

journal_volume

104

pub_type

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