Abstract：
:During a 6-month period at Walter Reed Army Hospital the monthly attack rate of Staphylococcus aureus bacteremia increased to 3.8 +/- 0.5 (mean +/- SEM) from 2.5 +/- 0.2 cases per 1,000 dispositions for the previous 48 months (P less than 0.05). A predominant phage pattern, designated S, was found in 12 (39%) of 31 bacteremic isolates typed and another strain, delta, was associated with four catheter-related infections. Two other strains also accounted for infections. Patients with isolates of the S phage pattern had a higher mortality (59%) than patients with non-S isolates (37%). Thirty-eight per cent of S. aureus carriers among hospital personnel harbored S or delta strains. Limitation of intravascular devices, strict handwashing, and the use of gloves were associated with a significant decrease in the incidence of S. aureus bacteremia to 1.9 +/- 0.5/1,000 dispositions over the next 6 months (P less than 0.05). S and delta strains were reduced to 20% of these isolates despite their persistence in 32% of staphylococcal carriers upon reculture of personnel. We conclude that S. aureus persists as an important pathogen in the hospitals, and that phage typing S. aureus isolates remains an important tool in hospital epidemiology. The presence of multiple S. aureus strains causing this outbreak and the extent of their dissemination among patients and personnel reported here emphasizes the need to reevaluate strategies of nosocomial staphylococcal control.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Cross AS,Zierdt CH,Roup B,Almazan R,Swan JCdoi
10.1093/ajcp/79.5.598subject
Has Abstractpub_date
1983-05-01 00:00:00pages
598-603issue
5eissn
0002-9173issn
1943-7722journal_volume
79pub_type
杂志文章abstract：:To facilitate the differential diagnosis of poorly differentiated metastatic carcinomas of unknown primary site, we evaluated p63 and cytokeratin (CK) 5/6 as immunohistochemical markers for squamous cell carcinomas. The study cases were as follows: squamous cell carcinoma of the lungs, head/neck, esophagus, cervix ute...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/21TW-2NDG-JRK4-PFJX
更新日期：2001-12-01 00:00:00
abstract：:Reducing the rate of avoidable errors is crucial to patient safety. Telephone calls with misunderstood critical results constitute one area in which opportunities for improvement exist. The aviation industry has dealt with this issue by requiring pilots to repeat instructions received from the air traffic controller. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/9DYM-6R0T-M830-U95Q
更新日期：2004-06-01 00:00:00
abstract：:Urine dipstick jars often are left uncapped, which led the authors to wonder what effect prolonged air exposure might have on dipstick accuracy. Unexpired Ames Multistixs (Miles Inc., Elkhart, IN) were exposed to ambient air for intervals of up to eight weeks and were used to test urine for the presence or absence of ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.3.398
更新日期：1991-09-01 00:00:00
abstract：:The "floral" variant of follicle center lymphoma (FCL) may be confused with progressive transformation of germinal centers or lymphocyte predominance Hodgkin lymphoma. Immunohistochemistry and gene rearrangement studies are usually sufficient to differentiate among these entities. We present 11 cases of floral FCL tha...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/V6PJ-BDAP-F0LU-CB6T
更新日期：2000-12-01 00:00:00
abstract：:Acute paraplegia developed in a 53-year-old man with pulmonary aspergilloma because of contiguous extension of Aspergillus infection to the epidural and subdural spaces and spinal cord. Histopathologic findings of the spinal cord showed Aspergillus hyphae penetrating the myelin sheath and myelomalacia, predominantly i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.6.763
更新日期：1985-12-01 00:00:00
abstract：:The cases of three patients who had clinical courses and hematologic data consistent with plasma-cell leukemia or a terminal leukemic phase of multiple myeloma are presented. A double Bence Jones protein was demonstrated in the urine of two of the patients. Immunoelectrophoresis and immunofluorescence studies demonstr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.3.410
更新日期：1975-09-01 00:00:00
abstract：:The cases of 20 patients, each of whom has a positive lupus erythematosus cell preparation and a negative antinuclear factor test, are presented. The concept of a false-positive lupus erythematosus preparation is suggested. Five common mechanisms causing a false-negative antinuclear factor test are discussed and evalu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.3.495
更新日期：1976-09-01 00:00:00
abstract：:By electrophoresis, prealbumin is seen as a discrete band anodic to the albumin fraction. Cases of duplicate bands in the prealbumin region have been described previously in serum, cerebrospinal fluid, and urine, and have had an association with varied clinical entities. The authors describe a novel association of sev...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.4.475
更新日期：1994-04-01 00:00:00
abstract：:Examination of urinary sediment has been recommended as a useful diagnostic aid for renal amyloidosis. This procedure was evaluated in the cases of 57 patients, including 11 with proven renal amyloidosis and 13 considered very likely to have the disease. At least three types of urinary fibrillar material were observed...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.1.36
更新日期：1979-01-01 00:00:00
abstract：:We optimized fluorogenic thrombin-generation assays with regard to sample volume, calibration, analytic corrections, and activation reagents. Lower sample volumes (40 vs 80 microL) were associated with better recovery of thrombin activity, lower interference due to absorbance of light, and higher total thrombin genera...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP6AY4HTRAAJFQ
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVES:To examine carbonic anhydrase IX (CAIX), a marker of renal cell carcinoma that recently has been described in malignant effusions. METHODS:Pleural and peritoneal fluids with the following diagnoses-reactive (n = 23), carcinoma (n = 17), and "suspicious for mesothelioma" (n = 4)-were immunostained for CAIX, ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP8BJ8CJXUEHZD
更新日期：2014-07-01 00:00:00
abstract：:From November 1985 to December 1990, 2,552 endomyocardial biopsy specimens from 209 heart transplant patients were studied. Forty-four (21%) patients developed 45 episodes of major human cytomegalovirus infection (HCMV). Human cytomegalovirus infection was primary in 13 of 44 patients. Thirty-one patients developed ep...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:The cytodiagnosis of Pneumocystis carinii (PC) in bronchoalveolar lavage (BAL) fluids has traditionally required a special stain such as Gomori's methenamine silver (GMS) stain. Recent reports indicate that identification of foamy alveolar casts (FACs) with Papanicolaou's (Pap) stain may provide a sensitive and less c...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.583
更新日期：1991-04-01 00:00:00
abstract：:The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.6.687
更新日期：1990-12-01 00:00:00
abstract：:The expression of HLA class I antigens in testicular germ cell tumors (TGCTs) was studied by the immunoperoxidase technique. In the normal testicle, the interstitial cells of Leydig as well as most of the germ cells were significantly stained. In typical seminoma, 75% of the tumor cells in stage I and 30% in stage II ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.2.202
更新日期：1990-02-01 00:00:00
abstract：:Cell counter-based formulas have been used in the differential diagnosis of microcytic anemia. The measurement of RBC subpopulations is now available on the Sysmex XE 5000 analyzer (Sysmex, Kobe, Japan). We describe the new formulas: % microcytic - % hypochromic; and % microcytic - % hypochromic - red cell distributio...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPJRH1I0XTNFGA
更新日期：2011-03-01 00:00:00
abstract：:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.517
更新日期：1991-04-01 00:00:00
abstract：:Using digital cell image analysis performed on Feulgen-stained nuclei, the nuclear characteristics of prostatic neoplasia, ranging from benign (benign prostatic hyperplasia [BPH]), through dysplastic (prostatic intraepithelial neoplasia [PIN] 1-3), to carcinoma were studied. Four histopathologic groups were studied: g...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.5.628
更新日期：1991-11-01 00:00:00
abstract：:Determination of HER-2/neu oncogene amplification has become clinically important in the present management of breast cancer and may have important applications in other areas of clinical oncology and scientific research. In situ hybridization is an extremely accurate and sensitive technique for assessing amplificatio...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/p40p2ead42pukdmg
更新日期：2003-03-01 00:00:00
abstract：:The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPAH5WR2LFTUSI
更新日期：2011-12-01 00:00:00
abstract：:Immunohistochemistry occasionally is used to determine the lineage of entirely necrotic tumors. However, the sensitivity and specificity of antibodies on necrotic tissue are unknown. To determine the usefulness of immunohistochemistry with necrotic lesions, a series of 24 known tumors consisting of 14 carcinomas, 2 ly...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/110.5.641
更新日期：1998-11-01 00:00:00
abstract：:To assess the observation that blood type B might be a marker for longevity, we reviewed the records and determined the ABO blood types of all patients who died in our hospital in 2004. Age was stratified by decade of death, and linear regressions were calculated by ABO percentage. ABO survival curves were compared. I...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPMIHJ6L3RPHZX
更新日期：2011-01-01 00:00:00
abstract：:A multiplex polymerase chain reaction (PCR) assay using oligonucleotide primers to detect mecA and 16S ribosomal RNA gene was developed to aid in identification of methicillin-resistant staphylococci. Validation included 99 isolates of staphylococcus grouped into one of five categories: methicillin-susceptible coagula...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.3.368
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVES:Millions of biospecimens will be collected during the coronavirus disease 2019 (COVID-19) pandemic. As learned from severe acute respiratory syndrome (SARS), proper biospecimen handling is necessary to prevent laboratory-related infections. METHODS:Centers for Disease Control and Prevention and World Health...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/aqaa171
更新日期：2021-01-04 00:00:00
abstract：:Campylobacter medium (CAMPY, Becton Dickinson [BD] Microbiology Systems, Cockeysville, MD) and Vancomycin Screen Agar (VSA, BD) were compared for their ability to screen for vancomycin-resistant Enterococcus (VRE) from primary plates. A microdilution minimal inhibitory concentration (MIC) assay (Pasco, BD) served as t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TWY5-04QE-9KV0-52MT
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVES:Our aim was to investigate the frequency of steatohepatitic morphology in hepatocellular adenoma (HCA) and correlate with its clinical parameters and risk factors underlying nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH). METHODS:We examined a series of 41 liver resection sp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa075
更新日期：2020-09-08 00:00:00
abstract：:Monoclonal immunoradiometric assays (IRMA) for human chorionic gonadotropin (hCG) are available for either intact hCG (IhCG) or beta subunit hCG (beta hCG). The authors evaluated the clinical applications of both methods. Serum samples (N = 180) were divided into the following five clinical groups: Group 1: elevated l...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.3.347
更新日期：1990-03-01 00:00:00
abstract：:The reliability of "standard" plasmas used by clinical laboratories to assay factor VIII concentrations of patients' plasmas was studied by assessing the credibility of their reference values. Two of three commercial reference plasmas were considered to be unreliable. The third commercial reference plasma appeared to ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.2.197
更新日期：1981-02-01 00:00:00
abstract：:Plasma samples from patients with severe classic hemophilia, suspected of having mild inhibitors not detected in the standard Bethesda inhibitor test, were examined in modified Bethesda tests and in a screening test based on the activated partial thromboplastin time (APTT). The APTT test was more sensitive to mild inh...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/77.6.749
更新日期：1982-06-01 00:00:00
abstract：:A 55-year-old man presented with nerve compression and examination of tissue removed by laminectomy, and bone marrow aspiration was diagnostic of multiple myeloma. Protein studies showed a total serum protein of 5.7 g/dL, with a M-component in the fast beta region. The abnormal protein reacted only with anti-lambda an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
