Abstract：
OBJECTIVES:Millions of biospecimens will be collected during the coronavirus disease 2019 (COVID-19) pandemic. As learned from severe acute respiratory syndrome (SARS), proper biospecimen handling is necessary to prevent laboratory-related infections. METHODS:Centers for Disease Control and Prevention and World Health Organization severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interim biosafety guidelines continue to be updated. Presented here are additional considerations intended to complement the interim guidance. These considerations draw on prior SARS recommendations and recent COVID-19 reports. RESULTS:SARS-CoV-2 viral RNA has been detected in various biospecimen types; however, studies are needed to determine whether viral load indicates viable virus. Throughout the pandemic, biospecimens will be collected for various purposes from COVID-19 known and suspected cases, as well as presymptomatic and asymptomatic individuals. Current data suggest the pandemic start may be as early as October 2019; thus, all biospecimens collected since could be considered potentially infectious. CONCLUSIONS:All entities handling these biospecimens should do risk assessments in accordance with institutional policies and adhere to any guidance provided. The scientific community has a responsibility to safely handle and maintain all biospecimens collected during the COVID-19 pandemic. Soon, it will be imperative to convene expert working groups to address the current and long-term storage and use of these biospecimens. Ideally, worldwide guidelines will be established to protect the personnel handling these biospecimens and communities at large.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Lankes HA,Makhlouf Hdoi
10.1093/ajcp/aqaa171subject
Has Abstractpub_date
2021-01-04 00:00:00pages
55-63issue
1eissn
0002-9173issn
1943-7722pii
5911469journal_volume
155pub_type
杂志文章,评审abstract：:The immunochemical characterization of the light chains of the mesangial immunoglobulin A (IgA) deposits were studied in 45 patients with IgA nephropathy. Kappa and lambda light chains were detected with direct immunofluorescence (IF) method, using monospecific rabbit anti-human anti-kappa and anti-lambda anti-sera. T...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.5.548
更新日期：1986-05-01 00:00:00
abstract：:During a three-year period, Staphylococcus aureus and Pseudomonas aeruginosa were the organisms most commonly isolated from blood cultures of burned children. Microorganisms were considered to contribute to the cause of death in 17 of 20 patients who died from various complications of thermal injuries. Pseudomonas aer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/63.4.502
更新日期：1975-04-01 00:00:00
abstract：:Acute myeloid leukemia (AML) with normal cytogenetics represents approximately 40% to 50% of de novo AML. This heterogeneous AML subgroup constitutes the single largest cytogenetic group with an intermediate prognosis. Previous studies have suggested that the Fms-like tyrosine kinase-3 internal tandem duplication (FLT...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/NRTX9AKXHR5JBT93
更新日期：2008-04-01 00:00:00
abstract：:There is a need for a simple, sensitive, specific, and inexpensive test for immunoglobulin M antibody to hepatitis B core antigen (anti-HBc IgM). A solid phase passive hemagglutination test (SP-PHA) was developed for this purpose and compared with the enzyme-linked immunosorbent assay (ELISA) test. Hepatitis B core an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/87.2.267
更新日期：1987-02-01 00:00:00
abstract：:A case of Torulopsis glabrata endocarditis involving the mitral valve is reported. Endocarditis developed during convalescence from severe abdominal trauma and was associated with fungemia. Morphologically characteristic organisms were present in the mitral valve. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.3.420
更新日期：1978-09-01 00:00:00
abstract：:The cytologic features of fine-needle aspiration specimens from 16 breast carcinomas that closely simulated benign lesions were analyzed and compared to smears from fibroadenomas and fibrocystic change. No combination of features was found that accurately separated all benign and malignant cases. Many nuclei with disc...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/98.2.155
更新日期：1992-08-01 00:00:00
abstract：:The API-20 Enteric Strip has been proposed to have the capacity to differentiate the nonfermentative gram-negative bacilli. This multitest strip serves as the basis of an evolving system, which first provided a Register, then a Selector, and more recently a computer Index. Twenty-three strains of Pseudomonas aeruginos...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.1.41
更新日期：1978-01-01 00:00:00
abstract：:The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/YTU2-FUHB-EXJL-WMLD
更新日期：2003-10-01 00:00:00
abstract：:This study aimed to ascertain pathologic findings of surgical follow-up excision (FUE) on patients who had radiologic finding of calcifications and lobular neoplasia (LN) on core biopsy. Breast core biopsy specimens from 2006-2011 with a diagnosis of pure classic-type LN (lobular carcinoma in situ [LCIS] and atypical ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPYG48TUTFIBMR
更新日期：2012-07-01 00:00:00
abstract：:To facilitate the differential diagnosis of poorly differentiated metastatic carcinomas of unknown primary site, we evaluated p63 and cytokeratin (CK) 5/6 as immunohistochemical markers for squamous cell carcinomas. The study cases were as follows: squamous cell carcinoma of the lungs, head/neck, esophagus, cervix ute...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/21TW-2NDG-JRK4-PFJX
更新日期：2001-12-01 00:00:00
abstract：:Although reports have suggested the incomplete type of intestinal metaplasia (IM) had a close correlation with carcinoma, considerable data showed no apparent relationship between the particular type of IM and the intestinal type carcinoma. The purpose of this study was to establish a novel classification of IM using ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.2.181
更新日期：1998-02-01 00:00:00
abstract：OBJECTIVES:Method imprecision, error rates, and explanatory causes that were identified in the Institute for Quality Management in Healthcare point-of-care (POC) glucose proficiency testing (PT) program were assessed in comparison with results obtained from laboratory glucose PT surveys. METHODS:POC and laboratory glu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP5YS2MVSKBYY
更新日期：2014-12-01 00:00:00
abstract：:Two hundred serum samples were tested with two assays for rapid detection of cytomegalovirus (CMV) antibodies. The assays were CMV Scan, a two-step latex particle agglutination assay for CMV IgG and IgM antibodies, and TranSTAT-CMV, a newly available six-step solid-phase enzyme immunoassay for CMV IgG antibodies. Comp...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.5.539
更新日期：1993-05-01 00:00:00
abstract：OBJECTIVES:To determine the public health surveillance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing volume needed, both for acute infection and seroprevalence. METHODS:Required testing volumes were developed using standard statistical methods based on test analytical performance, disease preval...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa099
更新日期：2020-07-07 00:00:00
abstract：:We conducted this study to ascertain the prevalence of erb-b2 gene amplification in breast cancer specimens read as 2+ in immunohistochemical analysis. Slides from patients with metastatic or recurrent breast cancer were eligible for fluorescent in situ hybridization (FISH) study if they were read as 2+ immunohistoche...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/R2X4KK22QCL7PLME
更新日期：2005-07-01 00:00:00
abstract：:Automation was introduced into the clinical microbiology laboratory in the 1960s but initially met with limited success. Today, instruments are an integral part of many clinical laboratories and are used for microbial detection, identification, and susceptibility testing; detection of positive blood cultures; screenin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1992-10-01 00:00:00
abstract：:Two commercial enzyme-linked immunosorbent assays (ELISA) for detection of herpes simplex virus (HSV) antigen in direct clinical specimens were compared with cell culture in primary rabbit kidney and MRC-5. With a total of 238 specimens, the sensitivity and specificity of the Dako ELISA method were determined to be 70...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.2.229
更新日期：1986-02-01 00:00:00
abstract：:Proliferative lesions, which include collagen, elastic fibers and abnormally oriented muscle fibers, were found to develop in intramyocardial arteries more rapidly with age in cigarette smokers than in nonsmokers. The study attempted to determine whether these lesions reduce perfusion pressures in the subendocardial z...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/68.4.493
更新日期：1977-10-01 00:00:00
abstract：OBJECTIVES:Preanalytical factors, such as hemolysis, affect many components of a test panel. Machine learning can be used to recognize these patterns, alerting clinicians and laboratories to potentially erroneous results. In particular, machine learning might identify which cases of elevated potassium from a point-of-c...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa039
更新日期：2020-07-07 00:00:00
abstract：OBJECTIVES:BCOR-CCNB3 sarcoma (BCS) is an undifferentiated tumor that has some clinical and morphologic similarity to classic Ewing sarcoma, but it is characterized by a distinct BCOR-CCNB3 gene fusion. There are no reports describing its cytomorphologic findings. METHODS:We describe cytologic findings of five molecul...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz159
更新日期：2020-02-08 00:00:00
abstract：Objectives:To analyze interobserver reproducibility and compare practice patterns between academic and community settings of Lower Anogenital Squamous Terminology (LAST). Methods:In total, 132 anal biopsy slides were revised as well as p16 immunostains. Results:LAST was used in 49% of cases (academic center, 68%; sat...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqx044
更新日期：2017-07-01 00:00:00
abstract：:New federal regulations require review of all available negative gynecologic smears within the previous 5 years for each patient with a current high grade squamous intraepithelial lesion (HGSIL) or above. Two university hospitals retrospectively reviewed all patients who had HGSIL or above and who had prior negative s...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.1.19
更新日期：1994-01-01 00:00:00
abstract：:p53 protein expression was evaluated in a series of 204 primary colorectal adenocarcinomas by immunohistochemistry using frozen tissue sections and monoclonal antibody DO-7. Nuclear staining of more than 5% of neoplastic cells was observed in 124 (60.8%) adenocarcinomas, which were classified as p53 positive. p53 immu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/105.5.604
更新日期：1996-05-01 00:00:00
abstract：:The uptake and storage of 5-hydroxytryptamine by human platelets from normal donors and patients with the carcinoid syndrome are reported. When platelets were incubated in the presence of 3H-5-hydroxytryptamine (50 microM), an initial phase of rapid uptake was observed until 5-hydroxytryptamine levels of 1--2 microgra...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.4.628
更新日期：1978-10-01 00:00:00
abstract：:A case of a patient with otherwise typical multiple myeloma whose myeloma cells were phagocytic is described. Phagocytosis of erythrocytes, erythrocytic precursors, lymphocytes, and platelets was found. Ingestion of latex particles by myeloma cells was demonstrated in vitro. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.6.722
更新日期：1979-06-01 00:00:00
abstract：OBJECTIVES:To examine laser microdissection and mass spectrometry (LMD-MS), which has emerged as a new tool to aid in typing amyloid proteins. RESULTS:ALECT-2 is a potential cause of hepatic amyloidosis best detected by LMD-MS. METHODS:One of the more recently reported proteins is ALECT-2 (leukocyte cell-derived chem...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPCLK54RKXTRDI
更新日期：2014-02-01 00:00:00
abstract：:Anaerobiospirillum succiniciproducens is a little-known spiral bacterium. Presented here are two septicemia cases similar in that both occurred in rural northern Virginia, both patients presented with dental infection, and in both cases the organism was first suspected to be Campylobacter. The morphology, motility, bi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.1.73
更新日期：1986-01-01 00:00:00
abstract：:Immunohistochemistry occasionally is used to determine the lineage of entirely necrotic tumors. However, the sensitivity and specificity of antibodies on necrotic tissue are unknown. To determine the usefulness of immunohistochemistry with necrotic lesions, a series of 24 known tumors consisting of 14 carcinomas, 2 ly...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/110.5.641
更新日期：1998-11-01 00:00:00
abstract：:In diagnostic pathology, decision-making skills are used to match the facts of a particular case to a diagnostic category. Ideally the diagnosis is established with "beyond a reasonable doubt" certainty, but substantial uncertainty orfrank diagnostic error can afflict the diagnostic process for a variety of reasons. M...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/YUCA-HMEE-BKQ5-BH5B
更新日期：2001-12-01 00:00:00
abstract：:We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
