Simplified neutrophil hexose monophosphate shunt assay on whole blood.

Abstract:

:Neutrophil function was determined in resting and phagocytic cells by an assay of hexose monophosphate shunt activity performed on small volumes of whole blood. This new micromethod clearly differentiated between zymosan-stimulated and unstimulated hexose monophosphate shunt activity. Furthermore, the whole blood microassay was able to measure the large quantities of 14CO2 that previously have been measured by hexose monophosphate shunt assays performed on erythrocyte-free leukocyte preparations. Thus, because of its simplicity and accuracy, the microassay described in this report can be used for routine evaluation of those metabolic activities of neutrophils associated with phagocytosis and bactericidal activity.

journal_name

Am J Clin Pathol

authors

King JW,McCall CE

doi

10.1093/ajcp/74.1.88

subject

Has Abstract

pub_date

1980-07-01 00:00:00

pages

88-93

issue

1

eissn

0002-9173

issn

1943-7722

journal_volume

74

pub_type

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