Rapid immunocytochemical analysis of acute leukemias.

abstract：:The expression of HLA class I antigens in testicular germ cell tumors (TGCTs) was studied by the immunoperoxidase technique. In the normal testicle, the interstitial cells of Leydig as well as most of the germ cells were significantly stained. In typical seminoma, 75% of the tumor cells in stage I and 30% in stage II ...

journal_title：American journal of clinical pathology

authors： Klein B,Klein T,Konichezky M,Nyska A,Livini E,Levine I,Zamir R,Kooperman O,Lurie H

更新日期：1990-02-01 00:00:00

abstract：:In 42 patients with locally advanced breast cancer treated with neoadjuvant chemotherapy followed by surgery and radiation therapy, the effects of chemotherapy on tumor architecture, morphometric nuclear and nucleolar characteristics, DNA ploidy, proliferation index measured by mitotic activity index, expression of di...

journal_title：American journal of clinical pathology

authors： Honkoop AH,Pinedo HM,De Jong JS,Verheul HM,Linn SC,Hoekman K,Wagstaff J,van Diest PJ

更新日期：1997-02-01 00:00:00

abstract：:The demonstration of tartrate-resistant acid phosphatase (TRAP) activity has long been a cornerstone in the diagnosis of hairy cell leukemia (HCL). Recently a monoclonal antibody to this enzyme has been developed that can be used in an immunoperoxidase method on paraffin-embedded tissues. By using a peroxidase-labeled...

journal_title：American journal of clinical pathology

authors： Hoyer JD,Li CY,Yam LT,Hanson CA,Kurtin PJ

更新日期：1997-09-01 00:00:00

abstract：:Peripheral blood samples of 23 patients who had hairy cell leukemia were examined for leukocyte alkaline phosphatase scores. Leukocyte alkaline phosphatase levels were elevated in seventeen patients (74%), were normal in six (26%), and were not decreased in any patient. The mean leukocyte alkaline phosphatase score wa...

journal_title：American journal of clinical pathology

authors： Aiba M,Raffa PP,Katayama I

更新日期：1980-09-01 00:00:00

abstract：:The present immunoperoxidase study attempted to localize aldosterone and cortisol in formalin-fixed paraffin-embedded adrenal glands or tumors. Apparently, steroid substances are solubilized during routine tissue processing, and the cytoplasm of adrenal cortical cells is not stained by either antialdosterone or antico...

journal_title：American journal of clinical pathology

authors： Hsu SM,Raine L,Martin HF

更新日期：1981-01-01 00:00:00

abstract：:Nine human exocrine pancreatic adenocarcinomas were examined by serial sectioning and double- and triple-labeled immunohistochemical techniques with antibodies against chromogranin A, insulin, islet amyloid polypeptide, glucagon, somatostatin, pancreatic polypeptide, serotonin, pancreastatin, and neuron-specific enola...

journal_title：American journal of clinical pathology

authors： Pour PM,Permert J,Mogaki M,Fujii H,Kazakoff K

更新日期：1993-09-01 00:00:00

abstract：:The present study evaluated the origin of endothelial and epithelial cells, as well as of lymphocytes and macrophages, after lung transplantation. Biopsy specimens from patients who underwent lung and heart-lung transplantation and received organs of sex-mismatched donors were studied by means of nonisotopic in situ h...

journal_title：American journal of clinical pathology

authors： Bittmann I,Dose T,Baretton GB,Müller C,Schwaiblmair M,Kur F,Löhrs U

更新日期：2001-04-01 00:00:00

abstract：:Demonstration of prostate-specific acid phosphatase by immunologic methods in tissue sections or in plasma necessitates a monospecific antiserum. This is produced by immunizing rabbits with pure prostate-specific acid phosphatase antigen, prepared from seminal fluid. The ejaculate is centrifuged, dialyzed against a ci...

journal_title：American journal of clinical pathology

authors： de Vries GP,Slob AW,Jöbsis AC,Meijer AE,Sanders GT

更新日期：1979-12-01 00:00:00

abstract：:We studied the histologic and multiparameter flow cytometry (MFC) features of 12 cases of angioimmunoblastic T-cell lymphoma (AITL), 13 of mature T-cell lymphoma, and 25 control cases of reactive lymphoid hyperplasia to evaluate the role of CD10 in the differential diagnosis of peripheral T-cell lymphomas (PTCLs). A c...

journal_title：American journal of clinical pathology

authors： Stacchini A,Demurtas A,Aliberti S,Francia di Celle P,Godio L,Palestro G,Novero D

更新日期：2007-11-01 00:00:00

abstract：:We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-...

journal_title：American journal of clinical pathology

authors： Harenberg J,Wang L,Hoffmann U,Huhle G,Feuring M

更新日期：2001-03-01 00:00:00

abstract：:The pattern of spurious red blood cell parameters caused by serum cold agglutinins has been well characterized for the Coulter counters but not the Ortho ELT-8. The pattern consists of a spurious increase in mean corpuscular volume (MCV), a discrepancy between the hemoglobin and the hematocrit, the latter falsely redu...

journal_title：American journal of clinical pathology

authors： Solanki DL,Blackburn BC

更新日期：1985-02-01 00:00:00

abstract：:This is apparently the second report of a case of human meningoencephalitis caused by the nematode Micronema deletrix. The patient had severe granulomatous meningoencephalitis with numerous foci of hemorrhage and encephalomalacia. Numerous adult female and larval M. deletrix were in the brain. The organisms probably i...

journal_title：American journal of clinical pathology

authors： Shadduck JA,Ubelaker J,Telford VQ

更新日期：1979-10-01 00:00:00

abstract：:Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...

journal_title：American journal of clinical pathology

authors： Wang E,Boswell E,Siddiqi I,Lu CM,Sebastian S,Rehder C,Huang Q

更新日期：2011-02-01 00:00:00

abstract：:We analyzed the value in cervical cytology of a recently developed technique by which it is possible to remove thick tissue specimens, called microbiopsies, from cervical smears and to process them for histologic examination. In 12 (48%) of 25 cervical smears in which microbiopsies were found, the histologic sections ...

journal_title：American journal of clinical pathology

authors： Mravunac M,Verbeek DH,Sanders-Eras M,Besseling R,Wijnen-Dubbers C,Smedts F

更新日期：1997-08-01 00:00:00

abstract：OBJECTIVES:To examine high expression of tumor necrosis factor ligand superfamily member 13 (TNFSF13), which is correlated with several malignancies. METHODS:TNFSF13 messenger RNA expression in tumor cells and fibroblasts in a cohort of patients with non-small cell lung cancer (NSCLC) was analyzed by quantitative real...

journal_title：American journal of clinical pathology

authors： Qian Z,Qingshan C,Chun J,Huijun Z,Feng L,Qiang W,Qiang X,Min Z

更新日期：2014-02-01 00:00:00

abstract：:Absolute lymphocytosis after nonsurgical trauma was investigated in three patient groups at an acute-care tertiary referral hospital. The first group, with mild-to-moderate trauma, consisted of 64 patients who survived knife wounds to the chest and abdomen. Thirteen of the 64 patients had admission lymphocyte counts g...

journal_title：American journal of clinical pathology

authors： Thommasen HV,Boyko WJ,Montaner JS,Russell JA,Johnson DR,Hogg JC

更新日期：1986-10-01 00:00:00

abstract：:Immunostaining of cell cycle-related antigens, especially Ki-67, DNA polymerase alpha, and proliferating cell nuclear antigen, has become an important method to assess the proliferative activity of tumors. These three nuclear antigens were studied by immunohistochemical analysis of cytologic smears. These smears were ...

journal_title：American journal of clinical pathology

authors： Sasano H,Miyazaki S,Nishihira T,Sawai T,Nagura H

更新日期：1992-08-01 00:00:00

abstract：:Mycobacterium leprae causes leprosy. M leprae strains collected worldwide have been genetically clonal, which poorly explains the varying severity and clinical features of the disease. We discovered a new Mycobacterium species from 2 patients who died of diffuse lepromatous leprosy (DLL). The Mycobacterium was purifie...

journal_title：American journal of clinical pathology

authors： Han XY,Seo YH,Sizer KC,Schoberle T,May GS,Spencer JS,Li W,Nair RG

更新日期：2008-12-01 00:00:00

abstract：:Two atypical human non-Hodgkin's lymphomas (NHLs) that exhibited unusual genotypic and in situ immunophenotypic abnormalities are described. Immunophenotypically, both NHLs lacked surface Ig heavy chains. With the exception of the MB2 B-cell-associated antigen, no B- and T-cell differentiation antigen was detected in ...

journal_title：American journal of clinical pathology

authors： De Re V,Carbone A,Dolcetti R,Tirelli U,Folegotto C,Boiocchi M

更新日期：1990-10-01 00:00:00

abstract：OBJECTIVES:Pathogenic free-living amebae (FLAs) cause skin, ocular, and central nervous system (CNS) infections with significant morbidity and mortality. Diagnosis of FLA infections by pathologic examination of tissue sections can be aided using molecular assays. This study investigated the performance characteristics ...

journal_title：American journal of clinical pathology

authors： Norgan AP,Sloan LM,Pritt BS

更新日期：2019-11-04 00:00:00

abstract：:The greater precision in prothrombin time monitoring obtained using thromboplastins with low international sensitivity index (ISI) values are believed to result in improved patient care. The authors conducted a blinded prospective study of 84 random patients on low-intensity warfarin therapy who were monitored with ei...

journal_title：American journal of clinical pathology

authors： Brophy MT,Fiore LD,Lau J,Goodwin R,Lopez A,Deykin D

更新日期：1994-07-01 00:00:00

abstract：:We retrospectively reviewed the immunophenotypic profile of 75 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) analyzed by flow cytometry. All patients had monoclonal IgM (median, 2,100 mg/dL [21 g/L]) in serum and were considered clinically to have WM. The neoplastic cells, in all cases, ex...

journal_title：American journal of clinical pathology

authors： Konoplev S,Medeiros LJ,Bueso-Ramos CE,Jorgensen JL,Lin P

更新日期：2005-09-01 00:00:00

abstract：:Chemotherapy is a well-established therapeutic approach for several malignancies, but its clinical efficacy is often limited by its related cardiotoxicity, which leads to cardiomyopathy, possibly evolving into heart failure. To detect cardiac damage, the adopted diagnostic approach is the estimation of left ventricula...

journal_title：American journal of clinical pathology

authors： Dolci A,Dominici R,Cardinale D,Sandri MT,Panteghini M

更新日期：2008-11-01 00:00:00

abstract：:Human serum contains 2 isoforms of type-5 tartrate-resistant acid phosphatase (TRACP): 5a and 5b. TRACP-5b is osteoclastic. Our goal was to determine if serum TRACP-5a could originate from inflammatory macrophages (MPhi). We stained 246 paraffin-embedded tissue samples for TRACP using monoclonal antibody 9C5 (mab9C5) ...

journal_title：American journal of clinical pathology

authors： Janckila AJ,Slone SP,Lear SC,Martin A,Yam LT

更新日期：2007-04-01 00:00:00

abstract：OBJECTIVES:We aimed to find descriptors to identify chronic lymphocytic leukemia (CLL), Sézary, granular, and villous lymphocytes among normal and abnormal lymphocytes in peripheral blood. METHODS:Image analysis was applied to 768 images from 15 different types of lymphoid cells and monocytes to determine four discrim...

journal_title：American journal of clinical pathology

authors： Puigví L,Merino A,Alférez S,Boldú L,Acevedo A,Rodellar J

更新日期：2019-06-05 00:00:00

abstract：:Specific esterase activity, a property generally ascribed to cells of granulocytic origin, was found in abnormal erythroid precursors obtained from the bone marrows of six patients with untreated erythroleukemia. These erythroblasts also showed nonspecific esterase activity. Neither specific nor nonspecific esterase a...

journal_title：American journal of clinical pathology

authors： Kass L

更新日期：1977-04-01 00:00:00

abstract：:Two patients with essential thrombocythemia showed a marked decrease in ristocetin-induced platelet aggregation in addition to other aggregation defects during routine aggregation studies for prolonged bleeding times. Further investigation revealed type I von Willebrand's disease (vWD) in one patient and a variant of ...

journal_title：American journal of clinical pathology

authors： Raman BK,Sawdyk M,Saeed SM

更新日期：1987-07-01 00:00:00

abstract：:The presence of small gas bubbles in devices used to collect blood gas samples is considered. A gas bubble whose relative volume is 0.5 to 1% or more that of the liquid in the collection device is a potential source of significant error. Relationships describing the possible magnitude of this error are derived and sub...

journal_title：American journal of clinical pathology

authors： Mueller RG,Lang GE,Beam JM

更新日期：1976-02-01 00:00:00

abstract：:The platelet aggregation responses induced by adenosine diphosphate (ADP) and collagen were characterized by parameters defining rate and extent of reaction. Values were compared on the basis of platelet concentration, time elapsed between sample collection and test performance, and sex of donors. Rate and extent of a...

journal_title：American journal of clinical pathology

authors： Roper P,Drewinko B,Hasler D,Johnston D,Hester J,Freireich EJ

更新日期：1979-03-01 00:00:00

abstract：:There are wide variations in the preparation of histologic sections from endoscopic esophageal biopsy specimens. We evaluated serial step sections from 261 esophageal or gastroesophageal junction biopsies at 4 levels to determine the first level at which goblet cell metaplasia (GCM) was detected. Deeper step sections ...

journal_title：American journal of clinical pathology

authors： Chitkara YK,Eyre CL

更新日期：2005-06-01 00:00:00