Abstract:
:Borrelia burgdorferi is difficult to detect in routine biopsy material from patients with skin lesions of borreliosis. In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to detect B burgdorferi in tissue sections and was compared with polymerase chain reaction (PCR). By using standard histologic equipment, tissue sections stained with a polyclonal B burgdorferi antibody were simultaneously scanned through 2 planes: horizontally in serpentines and vertically by focusing through the thickness of the section. Borrelia were detected in 47 of 71 ticks, 34 of 66 tick bites, 30 of 32 erythema chronicum migrans cases, 41 of 43 borrelial lymphocytomas, and 50 of 51 acrodermatitis chronica atrophicans cases. FFM proved to be more sensitive than PCR (96.0% vs 45.2%) and nearly equally specific (99.4% vs 100%). All 169 control cases, except 1 false-positive case of secondary syphilis, were negative with FFM. FFM is an easy, quick, and inexpensive method to reliably detect Borrelia in cutaneous tissue sections.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Eisendle K,Grabner T,Zelger Bdoi
10.1309/3369XXFPEQUNEP5Csubject
Has Abstractpub_date
2007-02-01 00:00:00pages
213-22issue
2eissn
0002-9173issn
1943-7722pii
M27475U16356W058journal_volume
127pub_type
杂志文章abstract::During a 6-month period at Walter Reed Army Hospital the monthly attack rate of Staphylococcus aureus bacteremia increased to 3.8 +/- 0.5 (mean +/- SEM) from 2.5 +/- 0.2 cases per 1,000 dispositions for the previous 48 months (P less than 0.05). A predominant phage pattern, designated S, was found in 12 (39%) of 31 ba...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.5.598
更新日期:1983-05-01 00:00:00
abstract::Fine-needle cytology (FNC) of 292 palpable orbital and eyelid tumors was performed with a 25-gauge (0.5-mm), 3-cm needle and compared with the histopathologic findings in 286 cases. Among these 286 cases, a concordant diagnosis of malignancy and type was achieved in 249 cases (87%). False positive diagnoses were made ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/93.1.100
更新日期:1990-01-01 00:00:00
abstract::Myofibromatosis generally is regarded as a fibrous proliferative disease of neonates and children that, even when multicentric, has an excellent prognosis and a propensity for spontaneous involution. Reported is a case of solitary, locally recurrent myofibromatosis in the ear canal of an adult patient. It was initiall...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.6.810
更新日期:1992-06-01 00:00:00
abstract::A survey to determine the need for training in medical mycology was sent to 605 US laboratories. Training needs were determined by comparing actual laboratory mycology practices with recommended practices, documenting the extent of mycology training reported by employees, and asking respondents to specify the fungi th...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/RR95-P7DN-41LW-1LGQ
更新日期:2002-08-01 00:00:00
abstract::Basaloid squamous cell carcinoma (BSCC) and carcinosarcoma of the esophagus are rare entities, making up fewer than 2% of esophageal malignancies. Comparative genomic hybridization (CGH) in 1 case of BSCC and 2 cases of carcinosarcoma and subsequent array CGH in 1 case each of BSCC and carcinosarcoma revealed common c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPZ1O7UUUISPNR
更新日期:2011-04-01 00:00:00
abstract::Vimentin has been regarded as the intermediate filament characteristic of normal and neoplastic mesenchymal cells and keratins as typical of epithelial cells and the neoplasms derived from them. However, many epithelial cells in tissue culture or in effusion, as well as cells in some solid epithelial neoplasms such as...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.3.286
更新日期:1987-09-01 00:00:00
abstract::Invasion of the visceral pleura is an important component of lung carcinoma staging, and in some studies is an independent prognostic indicator. Evaluation of invasion by H&E may be indeterminate. Elastic stains can be helpful but are performed rarely. We reviewed all lung carcinoma resections from 1993 for 13 histolo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/112.6.777
更新日期:1999-12-01 00:00:00
abstract::Thirty-five consecutive renal biopsy specimens were evaluated by light histology using paraffin and glycol methacrylate embedding. The clarity of the renal structures was found to be much better, and the renal lesions better defined, using glycol methyacrylate embedding. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.5.655
更新日期:1975-11-01 00:00:00
abstract::Cytogenetic studies of bone marrow were performed in 230 consecutive cases of patients with chronic granulocytic leukemia (CGL) admitted to the National Cancer Institute since 1961. Twenty patients lacked the Philadelphia (Ph1) chromosome. All were previously untreated. When compared with Ph1-positive patients, CGL pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.467
更新日期:1976-04-01 00:00:00
abstract::In-situ and microinvasive adenocarcinoma of the uterine cervix. A clinical, cytologic and histologic study of 14 cases. Am J Clin Pathol 64: 155-70, 1975. The clinicopathologic features of 14 cases of in-situ and microinvasive adenocarcinoma of the cervix, encountered during the period 1961-1974, form the basis of thi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.2.155
更新日期:1975-08-01 00:00:00
abstract::Terminal transferase (TdT) activity and antigen have been measured in 267 specimens of human bone marrow and peripheral blood by using a biochemical assay for enzymatic activity and an immunofluorescence test for antigen. Oligo p(dA)50 and dGTP were used as reagents in the biochemical assay and either rabbit anti-calf...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.4.420
更新日期:1982-04-01 00:00:00
abstract:OBJECTIVES:The 2014 Bethesda System (TBS 2014) guidelines for reporting cervical cytology revised the age for reporting benign endometrial cells (BECs) from 40 years or older to age 45 years or older. We evaluated this change and further investigated if extending the reporting age to 50 years or older may be acceptable...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa041
更新日期:2020-08-05 00:00:00
abstract::The present study evaluated the origin of endothelial and epithelial cells, as well as of lymphocytes and macrophages, after lung transplantation. Biopsy specimens from patients who underwent lung and heart-lung transplantation and received organs of sex-mismatched donors were studied by means of nonisotopic in situ h...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/GAFN-5MPA-LY8E-DTPQ
更新日期:2001-04-01 00:00:00
abstract::The authors analyzed the frequency of immunophenotypic abnormalities in 1,474 cases of routinely fixed, paraffin-embedded B-lineage non-Hodgkin's lymphomas. B-lineage was determined by immunoreactivity for CD20 (L26, 92%); CD45RA (4KB5, an additional 3%) or immunoglobulin (Ig) light chain restriction (remaining 5%). C...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.6.825
更新日期:1994-12-01 00:00:00
abstract::Diffuse alveolar damage (DAD) is usually considered a generalized lung process. During five years the authors observed 83 patients with generalized DAD in 827 adult autopsies (10.1%) and 10 patients with identical, but localized, lesions. The authors propose the term regional alveolar damage (RAD) to designate localiz...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/92.1.10
更新日期:1989-07-01 00:00:00
abstract::Patients with chronic renal failure (CRF) are at increased risk for myocardial events that are difficult to evaluate due to atypical symptoms and chronically elevated protein markers of cardiac damage. This study evaluated cardiac troponin T (cTnT), a sensitive marker of cardiac injury, in patients with CRF without my...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.1.118
更新日期:1996-07-01 00:00:00
abstract:OBJECTIVES:To support our Lean culture of continuous improvement, we implemented a daily management system designed so critical metrics of operational success were the focus of local teams to drive improvements. METHODS:We innovated a standardized visual daily management board composed of metric categories of Quality,...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPLQYMOFWU31CK
更新日期:2015-07-01 00:00:00
abstract::Immunohistochemical expression of napsin A in primary pulmonary mucinous tumors is not well established. Napsin A immunoreactivity was evaluated in 43 mucin-producing adenocarcinomas of the lung consisting of 18 tumors formerly classified as mucinous bronchioloalveolar carcinoma, 15 colloid adenocarcinomas, 5 solid pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP62WJUAMSZCOM
更新日期:2013-02-01 00:00:00
abstract::Certain new hematologic findings in eight homosexual or bisexual patients with the acquired immune deficiency syndrome (AIDS) are presented. All eight patients manifested a normochromic, normocytic anemia, and six of eight had granulocytopenia during their hospitalization. The other two had low-normal granulocyte coun...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.2.144
更新日期:1985-08-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00
abstract:OBJECTIVES:Universal screening of upper tract urothelial carcinoma (UTUC) for Lynch syndrome by mismatch repair (MMR) protein immunohistochemistry (IHC) has been recommended by some investigators. Herein, we assess this recommendation retrospectively by simulating its performance on a retrospective, unselected cohort o...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa100
更新日期:2020-11-04 00:00:00
abstract::Nineteen patients with acute nonlymphocytic leukemia were found to have a pericentric inversion of chromosome 16. All were classified as having acute myelomonocytic leukemia (M4) according to French-American-British Cooperative Group (FAB) criteria. Consistent abnormalities of marrow eosinophils were present. In 15 of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.6.733
更新日期:1984-06-01 00:00:00
abstract::Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.2.177
更新日期:1980-02-01 00:00:00
abstract::We studied the antigen expression profiles of 19 anaplastic large cell lymphoma (ALCL) cases by multiparameter flow cytometry. The neoplastic cells expressed CD45, HLA-DR, and CD30 in all cases. At least 1 T cell-associated antigen was expressed in each case (CD2, 12/17 [71%]; CD4, 12/19 [63%]; CD3, 6/19 [32%]; CD7, 6...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/HEFL-7KC4-35KF-WEX8
更新日期:2003-02-01 00:00:00
abstract::Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatog...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.1.89
更新日期:1995-07-01 00:00:00
abstract::This paper presents the first comprehensive morphometry analysis of normal thyroid, adenomas, and follicular and papillary carcinomas. The mean nuclear volume and the mean nuclear surface increased, while the volume densities of rough endoplasmic reticulum and dense bodies decreased from normal thyroid through adenoma...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.2.166
更新日期:1983-02-01 00:00:00
abstract::Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.2.237
更新日期:1984-02-01 00:00:00
abstract::A new generation hematology analyzer, Abbott CELL-DYN 4000 (CD 4000), capable of providing 26 parameters, including fully automated reticulocyte, nucleated RBC, blast, band, and immature granulocyte, and variant lymphocyte counts, was evaluated by using the National Committee for Clinical Laboratory Standards H20-A pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/L7LC-6KKJ-12G2-196W
更新日期:2000-04-01 00:00:00
abstract::Distributions of nine clinical chemistry determinations (serum bilirubin, globulin, creatinine, thyroxine, alkaline phosphatase, hematocrit, aspartate aminotransferase, uric acid, and plasma glucose) were assessed for 1,605 schoolchildren aged 6 to 17 years, in the Cincinati Lipid Research Clinic's Princeton School Di...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/74.4.371
更新日期:1980-10-01 00:00:00
abstract::The authors report here a case of lymphoepithelial cystic lesion (LECL) of unknown origin in the mediastinum, which is closely related to a signet-ring cell adenocarcinoma. A 73-year-old man presented with a mass as revealed on a chest x-ray. During surgical operation, a solid, well-circumscribed and encapsulated 9 X ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.6.808
更新日期:1989-12-01 00:00:00