Focus floating microscopy: "gold standard" for cutaneous borreliosis?

Abstract:

:Borrelia burgdorferi is difficult to detect in routine biopsy material from patients with skin lesions of borreliosis. In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to detect B burgdorferi in tissue sections and was compared with polymerase chain reaction (PCR). By using standard histologic equipment, tissue sections stained with a polyclonal B burgdorferi antibody were simultaneously scanned through 2 planes: horizontally in serpentines and vertically by focusing through the thickness of the section. Borrelia were detected in 47 of 71 ticks, 34 of 66 tick bites, 30 of 32 erythema chronicum migrans cases, 41 of 43 borrelial lymphocytomas, and 50 of 51 acrodermatitis chronica atrophicans cases. FFM proved to be more sensitive than PCR (96.0% vs 45.2%) and nearly equally specific (99.4% vs 100%). All 169 control cases, except 1 false-positive case of secondary syphilis, were negative with FFM. FFM is an easy, quick, and inexpensive method to reliably detect Borrelia in cutaneous tissue sections.

journal_name

Am J Clin Pathol

authors

Eisendle K,Grabner T,Zelger B

doi

10.1309/3369XXFPEQUNEP5C

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

213-22

issue

2

eissn

0002-9173

issn

1943-7722

pii

M27475U16356W058

journal_volume

127

pub_type

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