Abstract：
:Subsequent to recent disclosures that low serum vitamin B12 results can appear normal using many of the current radioassay methods because of the presence of cobalamin (vitamin B12) analogues in human serum, a number of radioassay kits have become available with modified intrinsic factor preparations and claims of measuring "true" vitamin B12. While evaluating two of these kits, one with R protein activity blocked and the other with R protein removed, the possibility was discovered that normal results could appear low owing to high nonspecific binding encountered with one of the methodologies. The problem of nonspecific binding was apparently due to incomplete inactivation of serum endogenous binding proteins and inadequate separation of free and bound radioisotope. The results demonstrate that "lower" vitamin B12 values are not necessarily "truer" values, and therefore caution and critical evaluation should temper the haste to change to commercial radioassay procedures that claim to provide "true" vitamin B12 levels.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
LeFebvre RJ,Virji AS,Mertens BFdoi
10.1093/ajcp/74.2.209subject
Has Abstractpub_date
1980-08-01 00:00:00pages
209-13issue
2eissn
0002-9173issn
1943-7722journal_volume
74pub_type
杂志文章abstract：:Monoclonal antibody (MoAb) 44-3A6 recognizes a glandular differentiation-associated antigen and has been used to identify exocrine differentiation in pulmonary carcinomas. The authors assessed its value in the diagnosis of lung carcinomas metastatic to lung/pleura and pleural malignant mesothelioma (MM), using cell bl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.3.322
更新日期：1991-03-01 00:00:00
abstract：:Carumonam, a new monobactam, was found to have an anti-microbial spectrum similar to aztreonam. Its spectrum includes Enterobacteriaceae, Haemophilus influenzae, pathogenic Neisseria species, Pseudomonas aeruginosa, and some streptococci. Staphylococcus species, enterococci, and many other nonenteric gram-negative bac...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.608
更新日期：1986-11-01 00:00:00
abstract：:Cytogenetic studies of bone marrow were performed in 230 consecutive cases of patients with chronic granulocytic leukemia (CGL) admitted to the National Cancer Institute since 1961. Twenty patients lacked the Philadelphia (Ph1) chromosome. All were previously untreated. When compared with Ph1-positive patients, CGL pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.4.467
更新日期：1976-04-01 00:00:00
abstract：:Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique wi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.4.517
更新日期：1991-04-01 00:00:00
abstract：:A study was made of quantitative urine cultures in bacteremic patients with urinary tract infection as the only identifiable source. These patients represent the most serious form of urinary tract infection and should be recognized as significant by the criteria used for interpreting quantitative urine cultures. In 83...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.5.616
更新日期：1986-05-01 00:00:00
abstract：:Three cases of microcystic adenomas of the pancreas with special reference to multicentric origin are described. The gross features and light microscopic findings were consistent with those described as being microcystic adenomas, but in two cases the gross examination and gelatin-embedded giant slices revealed multip...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.2.150
更新日期：1990-08-01 00:00:00
abstract：:Three policies for decreasing unnecessary cerebrospinal fluid (CSF) management Venereal Disease Research Laboratory (VDRL) tests were compared. The first policy attempted to educate physicians about the use of serologic tests for diagnosing neurosyphilis but allowed the CSF VDRL to be performed either as a screening t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.3.397
更新日期：1991-03-01 00:00:00
abstract：:Three cases of infantile hemangioendothelioma were immunohistochemically studied with the use of antibodies against von Willebrand factor (vWF), Ulex europaeus I lectin (UEA I), vimentin, thrombomodulin (TM), and actin, as endothelial cell (EC) markers. Because of a broad variety of histologic features, the growth pat...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.6.673
更新日期：1989-06-01 00:00:00
abstract：:Three anti-Factor-VIII antibodies from hemophiliacs were reacted with samples of batches of Maws commerical bovine and porcine Factor VIII concentrates manufactured over a 12-year period. The apparent antibody concentrations varied widely with the different batches of concentrates. The variations are probably due to i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.6.957
更新日期：1976-06-01 00:00:00
abstract：:We noticed almost routine disparate results in margin lengths when colorectal specimens are measured in vivo by the surgeon and in vitro by the pathologist. We studied 26 sigmoid and rectum specimens to document the amount of organ shrinkage after surgical removal and fixation. Each specimen had a 5.0-cm segment at ea...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.3.349
更新日期：1999-03-01 00:00:00
abstract：:We studied the expression of cytokeratin (CK) 7, CK20, CDX-2, and p16 in 119 cervical adenocarcinomas (65 usual type [50 invasive; 15 in situ], 37 intestinal type [21 invasive; 16 in situ], 10 endometrioid, 5 adenosquamous, and 2 signet-ring carcinomas) in comparison with 55 cases of rectal adenocarcinomas. The percen...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP7E5ASGOENPFP
更新日期：2009-10-01 00:00:00
abstract：:Platelet aggregation studies are usually performed in either electrical impedance or optical systems and the release reaction assessed by quantitating adenosine triphosphate (ATP) from luminesence produced by the firefly luciferin-luciferase system. In evaluating the results of such studies, attention is paid to a var...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.5.655
更新日期：1988-05-01 00:00:00
abstract：:The clinical and pathologic features of 64 inflammatory fibroid polyps of the large and small bowel in 63 patients are reported. Six of the lesions in the small bowel were studied by electron microscopy. Follow-up information was obtained from 39 patients. The lesion was always benign, occurred at all ages, and had a ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.708
更新日期：1984-06-01 00:00:00
abstract：:An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.3.337
更新日期：1986-03-01 00:00:00
abstract：:Blood gas measurements performed at the central laboratory and at a satellite laboratory of Brigham and Women's Hospital were studied to determine the differences in quality, turnaround time (TAT), and cost. The quality in both laboratories, as determined by results on proficiency and quality control samples, satisfac...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.1.7
更新日期：1994-07-01 00:00:00
abstract：:This study investigates the dispensing and inoculating characteristics of the MIC-2000 micro-broth dilution system and compares its ability to determine minimum inhibitory concentrations with that of the reference macro-broth dilution method. Micro-well filling showed a coefficient of variation of 6.8%, and micro-well...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.3.374
更新日期：1980-03-01 00:00:00
abstract：:The defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. The technic is based on the coupling of antigen or antibody with Sepharose 4B beads that have been activated by cyanogen bromide. In this study the activated beads were exposed to dengue antigen in the ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.3.343
更新日期：1978-09-01 00:00:00
abstract：:The ABH blood group isoantigen status of a retrospective series of 233 invasive breast carcinomas was examined, employing monoclonal antibodies (MCAB) against A, B, and H antigens with the avidin-biotin-peroxidase complex method. In addition, the H antigen was localized with Ulex Europeus Agglutinin I (UEAI) binding. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/83.3.308
更新日期：1985-03-01 00:00:00
abstract：:In the laboratory evaluation of platelet disorders, there are a number of situations in which determining the capacity of platelets to synthesize thromboxane is of diagnostic value. An assay for thromboxane production is required for the diagnosis of cyclooxygenase and thromboxane synthetase defects and is useful in d...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.2.216
更新日期：1988-02-01 00:00:00
abstract：:Pathologists are expected to accurately diagnose increasingly smaller breast carcinomas. Correct classification (ie, lobular vs ductal or in situ vs invasive) directly affects subsequent management, especially when the focus is near a surgical margin or present in a needle core biopsy and is further challenging if the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPF4O0ADKFMGRJ
更新日期：2013-01-01 00:00:00
abstract：:Tumor cells in the cerebrospinal fluid of an American child with Burkitt's lymphoma are described. The morphology of the cells was demonstrated better by use of the "millipore" technic and hematoxylin and eosin staining than by the Wright-stained smear. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.2.238
更新日期：1975-08-01 00:00:00
abstract：:The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.4.462
更新日期：1982-10-01 00:00:00
abstract：:To determine the current frequency of discovering important diagnoses at autopsy, the diagnoses made in all complete or "no head" autopsies during 1994 at a major tertiary care transplantation referral center were retrospectively compared with the diagnoses made antemortem. Of 176 autopsies, 79 (44.9%) revealed 1 or m...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/110.2.210
更新日期：1998-08-01 00:00:00
abstract：:Our aim was to study the possible association between oral lichen planus and chronic hepatitis C in patients from the state of Minas Gerais, Brazil. Two groups of patients were studied: group 1, 50 patients with oral lichen planus evaluated for the presence of chronic hepatitis C; and group 2, 215 patients with chroni...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/HDWCT36P0GMGP40V
更新日期：2007-05-01 00:00:00
abstract：:Anaerobiospirillum succiniciproducens is a little-known spiral bacterium. Presented here are two septicemia cases similar in that both occurred in rural northern Virginia, both patients presented with dental infection, and in both cases the organism was first suspected to be Campylobacter. The morphology, motility, bi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.1.73
更新日期：1986-01-01 00:00:00
abstract：:A new hemolytic plate method for the functional assessment of alternative-pathway complement activity is described. The plates are easy to prepare and the method simple to perform. Analysis of 23 normal serum samples showed a mean lysis diameter of 4.1 +/- 0.2 mm (1 standard deviation). ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.2.156
更新日期：1978-02-01 00:00:00
abstract：:A man was hospitalized with bacteremic Achromobacter xylosoxidans type IIIa pneumonia. The authors are aware of no previously reported similar infections caused by this bacterium. A clinical cure was achieved with a combination of carbenicillin and kanamycin therapy. Microtiter susceptibility testing revealed that car...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.4.712
更新日期：1978-10-01 00:00:00
abstract：:To our knowledge, there are no universally accepted, evidence-based guidelines for how to resolve the HER2 status of tumors demonstrating equivocal amplification. The present study was based on 17 breast core biopsy specimens demonstrating invasive carcinoma with equivocal HER2 amplification, defined as an HER2/chromo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/KFKDNK8CENVN24VU
更新日期：2008-03-01 00:00:00
abstract：:Wild-type apolipoprotein A-I (apo A-I)-derived amyloid commonly occurs in atherosclerotic plaques. To clarify apo A-I amyloid formation, plasma levels of apo A-I and cholesterol were related to the presence of amyloid in atherosclerotic plaques in 15 patients with peripheral atherosclerosis, subjected to arterial reco...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/PJE6-X9E5-LX6K-NELY
更新日期：2001-02-01 00:00:00
abstract：:Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/6H9844AMMET82DBJ
更新日期：2008-07-01 00:00:00
