Measuring HER-2 in breast cancer. Immunohistochemistry, FISH, or ELISA?

abstract：:Previous reports have indicated that patients with severe acute respiratory syndrome (SARS)-associated coronavirus infection could develop atypical pneumonia with fulminant pulmonary edema. However, the target cells of SARS viral infection have not been characterized in detail. We report the pathologic findings of the...

journal_title：American journal of clinical pathology

authors： Chow KC,Hsiao CH,Lin TY,Chen CL,Chiou SH

更新日期：2004-04-01 00:00:00

abstract：OBJECTIVES:To compare thromboelastography (TEG) tracings obtained from fresh and citrated whole-blood samples in patients on extracorporeal membrane oxygenation (ECMO) or after cardiopulmonary bypass and in healthy volunteers. METHODS:Samples of fresh and citrated whole blood were analyzed for 25 patients and 4 health...

journal_title：American journal of clinical pathology

authors： Gilman EA,Koch CD,Santrach PJ,Schears GJ,Karon BS

更新日期：2013-08-01 00:00:00

abstract：:Immunocytochemical assays for progesterone receptor (PR) using monoclonal antirabbit PR antibodies (PR-ICA) and for estrogen receptor (ER) (ER-ICA) were compared with radioligand binding (dextran-coated charcoal [DCC]) methods for receptor determination in patients with breast cancer. Immunocytochemical staining for P...

journal_title：American journal of clinical pathology

authors： Seymour L,Meyer K,Esser J,MacPhail AP,Behr A,Bezwoda WR

更新日期：1990-10-01 00:00:00

abstract：:A murine monoclonal antibody (MAb 12C3) that is specific to human ovarian carcinomas was generated by immunizing mice with a human ovarian germinoma cell line (JOHYC-2). The antigen distribution that was defined by MAb 12C3 in normal and malignant human tissues was analyzed by immunohistochemistry on paraffin-embedded...

journal_title：American journal of clinical pathology

authors： Yamada K,Ohkawa K,Joh K

更新日期：1995-03-01 00:00:00

abstract：:Cellular and humoral immunity combine to determine the outcome following exposure to hepatitis virus and are implicated in the proposed pathogenetic mechanism for acute and chronic hepatitis. Although antibody to HBsAg is found in virtually all following recovery from hepatitis B, a cell-mediated response to HBsAg can...

journal_title：American journal of clinical pathology

authors： Lee WM

更新日期：1976-05-01 00:00:00

abstract：OBJECTIVES:Millions of biospecimens will be collected during the coronavirus disease 2019 (COVID-19) pandemic. As learned from severe acute respiratory syndrome (SARS), proper biospecimen handling is necessary to prevent laboratory-related infections. METHODS:Centers for Disease Control and Prevention and World Health...

journal_title：American journal of clinical pathology

authors： Lankes HA,Makhlouf H

更新日期：2021-01-04 00:00:00

abstract：:p53 protein expression was evaluated in a series of 204 primary colorectal adenocarcinomas by immunohistochemistry using frozen tissue sections and monoclonal antibody DO-7. Nuclear staining of more than 5% of neoplastic cells was observed in 124 (60.8%) adenocarcinomas, which were classified as p53 positive. p53 immu...

journal_title：American journal of clinical pathology

authors： Lanza G Jr,Maestri I,Dubini A,Gafa R,Santini A,Ferretti S,Cavazzini L

更新日期：1996-05-01 00:00:00

abstract：OBJECTIVES:Bone marrow (BM) biopsy specimens involved by systemic mastocytosis (SM) typically show multifocal, compact, dense aggregates of spindled mast cells (MCs). However, some cases lack aggregate formation and fulfill the World Health Organization 2008 criteria for SM, based on minor criteria. METHODS:We identif...

journal_title：American journal of clinical pathology

authors： Reichard KK,Chen D,Pardanani A,McClure RF,Howard MT,Kurtin PJ,Wood AJ,Ketterling RP,King RL,He R,Morice WG,Hanson CA

更新日期：2015-09-01 00:00:00

abstract：:This is a report of an adenocarcinoma of mesonephric origin studied by light microscopy, electron microscopy, and immunocytochemistry. Unlike previous reports, our lesion was located paravaginally and not in the leaves of the broad ligament or in the cervix. The light microscopic features are similar to those of previ...

journal_title：American journal of clinical pathology

authors： Hinchey WW,Silva EG,Guarda LA,Ordonez NG,Wharton JT

更新日期：1983-10-01 00:00:00

abstract：:Immunohistochemical study of smooth muscle myosin, a protein distinct from skeletal, cardiac, or nonmyogenous myosins in paraffin-embedded normal tissues and benign and malignant mesenchymal tumors revealed its strong expression in normal smooth muscle, capillary endothelium, and pericytes. All benign smooth muscle tu...

journal_title：American journal of clinical pathology

authors： Donner L,de Lanerolle P,Costa J

更新日期：1983-11-01 00:00:00

abstract：:Abbreviated pretransfusion testing, although permitted by American Association of Blood Banks Standards for unimmunized patients, is not widely practiced. Concerns remain about optimal antibody screening methods, antibodies missed by deleting the antiglobulin crossmatch, and cost-effectiveness. The authors prospective...

journal_title：American journal of clinical pathology

authors： Cordle DG,Strauss RG,Snyder EL,Floss AM

更新日期：1990-10-01 00:00:00

abstract：:By studying more than 1,200 patients with cutaneous melanoma and long-term follow-up, I examined the relationship between solar elastosis and age at diagnosis of melanoma, key features of melanoma, and the outcome of overall survival in melanoma. I found that melanomas with elastosis were diagnosed significantly later...

journal_title：American journal of clinical pathology

authors： Vollmer RT

更新日期：2007-08-01 00:00:00

abstract：:A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...

journal_title：American journal of clinical pathology

authors： Warzynski MJ,Podgurski AE,Boldt DM,Hetzel PC

更新日期：1989-02-01 00:00:00

abstract：:Although prednisolone is the most common immunosuppressive agent used in renal transplantation, an accurate formulation of the optimal dose regimen remains to be established. A sensitive and precise radioimmunoassay was developed for this purpose. Fifty microliters of 1:20 dilutions of serum or 1:200 dilutions of urin...

journal_title：American journal of clinical pathology

authors： Woo J,Floyd M,Master G,Cannon DC

更新日期：1980-06-01 00:00:00

abstract：:Carumonam, a new monobactam, was found to have an anti-microbial spectrum similar to aztreonam. Its spectrum includes Enterobacteriaceae, Haemophilus influenzae, pathogenic Neisseria species, Pseudomonas aeruginosa, and some streptococci. Staphylococcus species, enterococci, and many other nonenteric gram-negative bac...

journal_title：American journal of clinical pathology

authors： Jones RN,Barry AL,Thornsberry C,Fuchs PC,Packer RR

更新日期：1986-11-01 00:00:00

abstract：:A case of epithelioid sarcoma in a 39-year-old woman who had metastatic lesions in her lung from a primary arm tumor is presented. A review of the literature and analysis of this case did not identify any specific light microscopic or immunohistochemical way to separate definitively the lung cytologic smear in this ca...

journal_title：American journal of clinical pathology

authors： Niemann TH,Bottles K

更新日期：1993-08-01 00:00:00

abstract：OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...

journal_title：American journal of clinical pathology

authors： Mathias PC,Conta JH,Konnick EQ,Sternen DL,Stasi SM,Cole BL,Astion ML,Dickerson JA

更新日期：2016-08-01 00:00:00

abstract：:A case of signet ring cell lymphoma, a rare variant of follicular center cell lymphoma, is presented with a review of 20 other such lymphomas previously reported in the English literature. The histologic, immunocytologic, and electron microscopic features are described with speculation as to the possible relationship ...

journal_title：American journal of clinical pathology

authors： Silberman S,Fresco R,Steinecker PH

更新日期：1984-03-01 00:00:00

abstract：:The Department of Laboratory Medicine, University of Washington, has explored various management of organizational approaches over the past seven years. The Centralized Processing Area supported by a computerized information system has evolved from these efforts. Despite a decrease in U.W. hospital usage since 1968-69...

journal_title：American journal of clinical pathology

authors： Nussbaum B,Minckler T,Roby R,Ackerman E

更新日期：1977-02-01 00:00:00

abstract：:Endometrial vascular changes similar to atherosclerosis of toxemia of pregnancy were described and graded in 217 consecutive endometrial biopsies of known early intrauterine pregnancy. Severe vascular changes were found in 23.5% of cases. Control material consisting of endometrial biopsies of patients with known cases...

journal_title：American journal of clinical pathology

authors： Lichtig C,Korat A,Deutch M,Brandes JM

更新日期：1988-09-01 00:00:00

abstract：:To evaluate the diagnostic utility of flow cytometry (FCM) as compared to cytology, DNA and RNA content of cells were measured in 233 samples of cerebrospinal fluid (CSF) from 147 patients with solid tumors or lymphomas and 17 controls with nonmalignant disorders. All control specimens were normal. Twenty-eight sample...

journal_title：American journal of clinical pathology

authors： Cibas ES,Malkin MG,Posner JB,Melamed MR

更新日期：1987-11-01 00:00:00

abstract：Objectives:To determine non-American Society for Clinical Pathology pathology- and laboratory-related Choosing Wisely recommendations that drive effective test utilization in the laboratory. Methods:Data were collected via a two-part web-based survey distributed to a broad sample of pathologists and laboratory profess...

journal_title：American journal of clinical pathology

authors： Waibel E,Garcia E,Kelly M,Soles R,Hilborne L

更新日期：2018-02-17 00:00:00

abstract：:The use of rapid, simple tests to detect antibodies to human immunodeficiency virus (HIV) in urine could be valuable for several testing situations, such as in private offices, for epidemiologic surveys, and in developing countries. The authors evaluated the performance of the SUDS HIV type 1 test to detect antibody t...

journal_title：American journal of clinical pathology

authors： Constantine NT,Zhang X,Li L,Bansal J,Hyams KC,Smialek JE

更新日期：1994-02-01 00:00:00

abstract：:The authors have assessed the clinical value of an "accurate" band count as a correlate to infection in 33 patients with a culture proven bacterial infection and a normal total leukocyte count. Thirty hematologically normal outpatients, presumably with no infections, were selected for the control group. Manual 500 cel...

journal_title：American journal of clinical pathology

authors： Ardron MJ,Westengard JC,Dutcher TF

更新日期：1994-11-01 00:00:00

abstract：:To assess the diagnostic accuracy of margin evaluation of melanocytic lesions using en face frozen sections compared with standard paraffin-embedded sections, we studied 2 sets of lesions in which en face frozen sections were used for analysis of surgical margins (13 from malignant melanomas [MMs] and 10 from nonmelan...

journal_title：American journal of clinical pathology

authors： Prieto VG,Argenyi ZB,Barnhill RL,Duray PH,Elenitsas R,From L,Guitart J,Horenstein MG,Ming ME,Piepkorn MW,Rabkin MS,Reed JA,Selim MA,Trotter MJ,Johnson MM,Shea CR

更新日期：2003-08-01 00:00:00

abstract：:The cases of three patients who had clinical courses and hematologic data consistent with plasma-cell leukemia or a terminal leukemic phase of multiple myeloma are presented. A double Bence Jones protein was demonstrated in the urine of two of the patients. Immunoelectrophoresis and immunofluorescence studies demonstr...

journal_title：American journal of clinical pathology

authors： Pedraza MA

更新日期：1975-09-01 00:00:00

abstract：:The aim of this study was to evaluate the histopathologic features and the expression of angiogenesis-related markers in primary tumors and metastatic lymph nodes of oral squamous cell carcinomas (SCCs) with multiple lymph node involvement in comparison with oral SCCs without nodal metastasis. The protein levels of th...

journal_title：American journal of clinical pathology

authors： Nikitakis NG,Rivera H,Lopes MA,Siavash H,Reynolds MA,Ord RA,Sauk JJ

更新日期：2003-04-01 00:00:00

abstract：Objectives:To analyze the economic impact of testing for activated protein C resistance (APC-R) due to factor V Leiden (FVL) mutation with APC-R with reflexive FVL genotyping (algorithmic approach) or genotyping alone. Methods:OptumLabs Data Warehouse (OLDW) data were used to assess testing approaches. Insurance claim...

journal_title：American journal of clinical pathology

authors： Perez Botero J,Majerus JA,Strege AK,Johnson RD,Chen D,Pruthi RK

更新日期：2017-06-01 00:00:00

abstract：OBJECTIVES:An intraoperative consultation (IOC) checklist was developed and implemented aimed at standardizing slide labeling and monitoring metrics central to quality and safety in surgical pathology. DESIGN:Data were collected for all IOC cases over a 9-month period. Slide labeling defect rates and IOC turnaround ti...

journal_title：American journal of clinical pathology

authors： Chen Y,Anderson KR,Xu J,Goldsmith JD,Heher YK

更新日期：2019-05-03 00:00:00

abstract：:The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...

journal_title：American journal of clinical pathology

authors： Kumar NB

更新日期：1984-01-01 00:00:00