Molecular and Immunohistochemical Analysis of Mucinous Cystic Neoplasm of the Liver.

Abstract:

OBJECTIVES:Mucinous cystic neoplasm of the liver is characterized by neoplastic mucinous and/or biliary epithelium surrounded by ovarian-type stroma. Immunohistochemical studies have shown that the ovarian-type stroma expresses estrogen receptor, suggesting potential hormonal responsiveness. The molecular biology of mucinous cystic neoplasm of the liver remains poorly studied. METHODS:Transcriptome sequencing and immunohistochemistry were performed on a series of mucinous cystic neoplasms. RESULTS:Mucinous cystic neoplasm of the liver exhibited significantly increased RNA expression of ovarian stromal markers WT1, PR, and ER2 and sex cord stromal markers SF-1, inhibin-α, and calretinin compared with nonneoplastic liver. Immunohistochemistry confirmed the RNA-level data. Evidence for sex hormone biosynthesis was identified by significant overexpression of multiple estrogen biosynthetic enzymes. Expression of 17β-hydroxysteroid dehydrogenase 1 was confirmed immunohistochemically. Pathway analysis also identified significant upregulation of the hedgehog and Wnt pathways and significant downregulation of T-helper 1 and T-helper 2 pathways. CONCLUSIONS:Mucinous cystic neoplasm of the liver recapitulates ovarian stroma at the morphologic, DNA, RNA, and protein levels. These data support the concept that this tumor likely arises from ectopic primitive gonadal tissue and/or stromal cells with capacity to transdifferentiate to ovarian cortical cells.

journal_name

Am J Clin Pathol

authors

Van Treeck BJ,Lotfalla M,Czeczok TW,Mounajjed T,Moreira RK,Allende DS,Reid MD,Naini BV,Westerhoff M,Adsay NV,Kerr SE,Rizvi SH,Smoot RL,Liu Y,Davila J,Graham RP

doi

10.1093/ajcp/aqaa115

subject

Has Abstract

pub_date

2020-11-04 00:00:00

pages

837-847

issue

6

eissn

0002-9173

issn

1943-7722

pii

5901027

journal_volume

154

pub_type

杂志文章
  • The development and testing of a laboratory information system-driven tool for pre-sign-out quality assurance of random surgical pathology reports.

    abstract::We describe the development and testing of a novel pre-sign-out quality assurance tool for case diagnoses that allows for the random review of a percentage of cases by a second pathologist before case verification and release of the final report. The tool incorporates the ability to record and report levels of diagnos...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPLN9DU9LNXSXA

    authors: Owens SR,Dhir R,Yousem SA,Kelly SM,Piccoli A,Wiehagen L,Lassige K,Parwani AV

    更新日期:2010-06-01 00:00:00

  • Penicillin susceptibility of Streptococcus pneumoniae in 1978. Screening for resistance by disk testing.

    abstract::Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.2.177

    authors: Maki DG,Helstad AG,Kimball JL

    更新日期:1980-02-01 00:00:00

  • Significance of leukocyte alkaline phosphatase in hairy cell leukemia.

    abstract::Peripheral blood samples of 23 patients who had hairy cell leukemia were examined for leukocyte alkaline phosphatase scores. Leukocyte alkaline phosphatase levels were elevated in seventeen patients (74%), were normal in six (26%), and were not decreased in any patient. The mean leukocyte alkaline phosphatase score wa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.3.297

    authors: Aiba M,Raffa PP,Katayama I

    更新日期:1980-09-01 00:00:00

  • Papillary thyroid carcinoma with heterotopic ossification is a special subtype with extensive progression.

    abstract::An immunohistochemical study was conducted of 108 papillary carcinoma cases, including 48 cases with intratumoral heterotopic ossification (IHO). In 48 cases, papillary carcinoma with IHO was accompanied by nodular fibrosis. Cases of papillary carcinoma with IHO or nodular fibrosis showed higher incidences of lymph no...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPQZQN50HKIAHA

    authors: Takeda M,Mikami T,Numata Y,Okamoto M,Okayasu I

    更新日期:2013-05-01 00:00:00

  • Comparison of the conventional lecture method and the self-instruction method for teaching clinical pathology.

    abstract::Two separate courses in clinical pathology were presented to two groups of second-year medical students. The first (large) group was taught by the conventional lecture method; the other (small) group, by a self-instructional method for roughly half of the course, followed by the conventional lecture method for the sec...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/70.6.847

    authors: Kao YS,Beeler MF,Strong JP

    更新日期:1978-12-01 00:00:00

  • The role of aspiration biopsy cytology in the diagnosis of pulmonary tuberculosis.

    abstract::The experience at one institution concerning the diagnosis of pulmonary tuberculosis by aspiration biopsy is reviewed. Twelve cases in which acid-fast bacilli were identified in pulmonary aspiration material are reported. The cytologic findings were confirmed by microbiologic culture in nine cases. The advantages of u...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.6.719

    authors: Robicheaux G,Moinuddin SM,Lee LH

    更新日期:1985-06-01 00:00:00

  • Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinically Important Bacteria and Yeasts.

    abstract:Objectives:A report on the multicenter evaluation of the Bruker MALDI Biotyper CA System (MBT-CA; Bruker Daltonics, Billerica, MA) for the identification of clinically important bacteria and yeasts. Methods:In total, 4,399 isolates of medically important bacteria and yeasts were assessed in the MBT-CA. These included ...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1093/ajcp/aqw225

    authors: Wilson DA,Young S,Timm K,Novak-Weekley S,Marlowe EM,Madisen N,Lillie JL,Ledeboer NA,Smith R,Hyke J,Griego-Fullbright C,Jim P,Granato PA,Faron ML,Cumpio J,Buchan BW,Procop GW

    更新日期:2017-06-01 00:00:00

  • A simple light-scattering method for detecting soluble immune complexes in human serum.

    abstract::A simple assay based on molecular light scattering was developed to detect soluble immune complexes. In this assay the percentage of relative light scattering (%RLS) caused by complexes in buffer containing polyethylene glycol 6000 (PEG) was measured with a laser nephelometer. In low concentrations of PEG (3%-5%), IgG...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.6.804

    authors: Koethe SM,Melton HE,Jendrzejczak Z

    更新日期:1980-12-01 00:00:00

  • An automated method for the analysis of T-cell receptor repertoires. Rapid RT-PCR fragment length analysis of the T-cell receptor beta chain complementarity-determining region 3.

    abstract::The examination of T-cell receptor (TCR) repertoires has an important role in the study of lymphoproliferative disorders and autoimmune diseases. Analysis of the complementarity-determining region 3 (CDR3) of the TCR beta chain is used to assess the clonality of T-cell populations. We developed a rapid fluorescence-ba...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/111.5.683

    authors: Lue C,Mitani Y,Crew MD,George JF,Fink LM,Schichman SA

    更新日期:1999-05-01 00:00:00

  • Clinical trial of a new disposable bleeding-time device.

    abstract::A new disposable bleeding time device (Hemalet) was tested in 20 normal individuals and 11 patients with various bleeding disorders. The results were compared with those of Simplate II. The mean bleeding time for normal individuals was 5.4 +/- 1.5 (mean +/- 1 SD) minutes by Hemalet and 5.8 +/- 1.4 (mean +/- 1 SD) minu...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章

    doi:10.1093/ajcp/85.5.610

    authors: Toomey KC,Kim HC,Kosmin M,Saidi P

    更新日期:1986-05-01 00:00:00

  • A rare lymphoepithelial cyst of the pancreas.

    abstract::Reported is a case of a rare lymphoepithelial cyst of the tail of the pancreas that developed in a young man with no symptoms. To the authors' knowledge, it is the first case described to be entirely situated within the pancreas, thus confirming its pancreatic origin. Biologic behavior appears to be entirely benign, a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/98.2.188

    authors: DiCorato MP,Schned AR

    更新日期:1992-08-01 00:00:00

  • The effects of heparin on lipoproteins in high-resolution electrophoresis of serum.

    abstract::Sera from heparinized patients commonly display anodal slurring of both the alpha and beta lipoproteins when they are examined by high-resolution electrophoresis (HRE). In this study, the authors examined the effect of heparin and lipoprotein lipase on the electrophoretic migration of alpha and beta lipoproteins in se...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/104.4.468

    authors: Pearson JP,Keren DF

    更新日期:1995-10-01 00:00:00

  • Analytic bias among certified methods for the measurement of hemoglobin A1c: a cause for concern?

    abstract::We studied the magnitude, significance, and origin of an analytic bias that emerged between our point-of-care (POC) and our central laboratory (CL) methods for the measurement of hemoglobin A1c (HbA1c) and evaluated the analytic accuracy of 7 commonly used HbA1c methods relative to the National Glycohemoglobin Standar...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/U3GPPTCBP1VLL8AW

    authors: Holmes EW,Erşahin C,Augustine GJ,Charnogursky GA,Gryzbac M,Murrell JV,McKenna KM,Nabhan F,Kahn SE

    更新日期:2008-04-01 00:00:00

  • Novel t(1;8)(p31.3;q21.3) NFIA-RUNX1T1 Translocation in an Infant Erythroblastic Sarcoma.

    abstract:OBJECTIVES:Pure erythroid leukemia (PEL) is exceptionally rare in the pediatric setting. Four pediatric PEL cases with t(1;16)(p31;q24) NFIA-CBFA2T3 were reported previously. We present a case of an infant with PEL presenting with erythroblastic sarcoma and harboring a novel t(1;8)(p31.3;q21.3) NFIA-RUNX1T1 fusion dete...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa216

    authors: King RL,Siaghani PJ,Wong K,Edlefsen K,Shane L,Howard MT,Reichard KK,Mai M,Viswanatha DS,Greipp PT,Goble TA,Ruiz M,Hara H

    更新日期:2020-12-14 00:00:00

  • Contemporary quality control practices for antimicrobial susceptibility tests: a report from the microbiology portion of the College of American Pathologists (CAP) surveys program.

    abstract::The evaluation of quality control (QC) practices from the College of American Pathologists (CAP) Microbiology Surveys demonstrates a trend away from daily controls similar to that first noted in 1980. Previous recommendations for more relevant cost-containment oriented weekly QC procedures are discussed, and the 1982 ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Jones RN,Edson DC,Gilmore BF

    更新日期:1983-10-01 00:00:00

  • Pathologic findings from the National Surgical Adjuvant Breast Project (protocol no. 4). VI. Invasive papillary cancer.

    abstract::Although papillary forms of intraductal cancer are a common component of amny mammary cancers, invasive papillary cancer of the breast is relatively rare. Only 35 examples were encountered in the specimens obtained from 1603 women participating in a prospective randomized study of invasive mammary cancer. Contingency-...

    journal_title:American journal of clinical pathology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1093/ajcp/73.3.313

    authors: Fisher ER,Palekar AS,Redmond C,Barton B,Fisher B

    更新日期:1980-03-01 00:00:00

  • Cytologic features of poorly differentiated 'insular' carcinoma of the thyroid, as revealed by fine-needle aspiration biopsy.

    abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.6.687

    authors: Pietribiasi F,Sapino A,Papotti M,Bussolati G

    更新日期:1990-12-01 00:00:00

  • Advantages of a new anticoagulant in routine hematology on the Coulter Counter S-Plus STKR analyzer.

    abstract::The authors report the advantages of a new anticoagulant-antiaggregant mixture that avoids the deleterious effects of ethylenediaminotetraacetic acid (EDTA) on mean platelet volume and also prevents EDTA-induced platelet clumping. It is suitable for routine cell counting and sizing with the Coulter Counter S-Plus STKR...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/93.6.760

    authors: Lippi U,Schinella M,Modena N,Nicoli M,Lippi G

    更新日期:1990-06-01 00:00:00

  • The clinical significance of eosinophiluria.

    abstract::Eosinophils are normally absent from the urine. Their presence has been associated with a variety of clinical conditions affecting the kidney or urinary tract. The authors examined the clinical significance and eosinophils, in particular, for acute interstitial nephritis. ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/88.4.520

    authors: Corwin HL,Haber MH

    更新日期:1987-10-01 00:00:00

  • Restoration of hemoglobin function in stored EDTA blood. Application in identification of hemoglobin variants with abnormal oxygen affinity.

    abstract::The incubation of stored blood in a mixture of inosine, pyruvate, glucose, and phosphate restores the O2 affinity of hemoglobin to physiologic levels, as measured by the configuration of the dissociation curve and the P50. This regeneration of normal hemoglobin function not only is consistent for samples anticoagulate...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/68.1.53

    authors: Sumoza A,Fairbanks VF,Pineda AA

    更新日期:1977-07-01 00:00:00

  • Bubbles in samples for blood gas determinations. A potential source of error.

    abstract::The presence of small gas bubbles in devices used to collect blood gas samples is considered. A gas bubble whose relative volume is 0.5 to 1% or more that of the liquid in the collection device is a potential source of significant error. Relationships describing the possible magnitude of this error are derived and sub...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1093/ajcp/65.2.242

    authors: Mueller RG,Lang GE,Beam JM

    更新日期:1976-02-01 00:00:00

  • Focus floating microscopy: "gold standard" for cutaneous borreliosis?

    abstract::Borrelia burgdorferi is difficult to detect in routine biopsy material from patients with skin lesions of borreliosis. In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to detect B burgdorferi in tissue sections and was compared with polymerase chain reaction (PCR). By usi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/3369XXFPEQUNEP5C

    authors: Eisendle K,Grabner T,Zelger B

    更新日期:2007-02-01 00:00:00

  • The medical usefulness of Stat tests.

    abstract::Although Stat tests are one of the most important features of clinical laboratory performance, the indexed literature is devoid of significant discussion of the subject. The authors, undertook a study by querying hospital laboratories about their current practices and received 38 useable answers dealing with the types...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.5.520

    authors: Barnett RN,McIver DD,Gorton WL

    更新日期:1978-05-01 00:00:00

  • Evaluation of real-time clinical decision support systems for platelet and cryoprecipitate orders.

    abstract:OBJECTIVES:To evaluate cryoprecipitate and platelet ordering practices after the implementation of real-time clinical decision support systems (CDSSs) in a computerized physician order entry (CPOE) system. METHODS:Uniform platelet and cryoprecipitate transfusion thresholds were implemented at 11 hospitals in a regiona...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,多中心研究

    doi:10.1309/AJCP1OSRTPUJE9XS

    authors: Collins RA,Triulzi DJ,Waters JH,Reddy V,Yazer MH

    更新日期:2014-01-01 00:00:00

  • Extensively keratinized squamous intraepithelial lesions of the cervix are difficult to grade.

    abstract::We tested the hypothesis that extensively keratinized squamous intraepithelial lesions (SILs) are difficult to grade precisely by identifying 100 Papanicolaou smears with a keratinizing SIL that had been originally judged difficult to grade. Of these, 65 were confirmed as low-grade SIL (LSIL) or high-grade SIL (HSIL) ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/RQBN-Y51J-CJTK-P9H5

    authors: Faquin WC,Brown FM,Krane JF,Renshaw AA,Cibas ES

    更新日期:2001-01-01 00:00:00

  • The cytopathology of proliferative breast disease. Comparison with features of ductal carcinoma in situ.

    abstract::Epithelial hyperplasia, a component of proliferative breast disease (PBD), is a known risk factor for the development of breast carcinoma. To determine if criteria established for tissue biopsy could be used to more precisely define cytologic criteria for diagnosis of PBD, 37 breast fine-needle aspirates with biopsy d...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/103.4.438

    authors: Dawson AE,Mulford DK,Sheils LA

    更新日期:1995-04-01 00:00:00

  • Platelet function, ultrastructure, and survival in the May-Hegglin anomaly.

    abstract::The May-Hegglin anomaly is one of the rare forms of heriditary thrombocytopenia. Since fewer than 100 cases of May-Hegglin anomaly have been described to date, major controversies regarding the adequacy of hemostasis in vivo and abnormalities of platelet function in vitro continue to prevail. This report describes the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.5.663

    authors: Hamilton RW,Shaikh BS,Ottie JN,Storch AE,Saleem A,White JG

    更新日期:1980-11-01 00:00:00

  • Pulmonary histologic changes in Marfan syndrome: a case series and literature review.

    abstract::Marfan syndrome is one of the most common connective tissue diseases and may manifest with a range of symptoms and pathologic changes. We present a retrospective series of 5 cases of patients with Marfan syndrome and pulmonary pathology. Patients were young to middle-aged adults with absent or minimal smoking historie...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/AJCP79SNDHGKQFIN

    authors: Dyhdalo K,Farver C

    更新日期:2011-12-01 00:00:00

  • Common genomic aberrations in basaloid squamous cell carcinoma and carcinosarcoma of the esophagus detected by CGH and array CGH.

    abstract::Basaloid squamous cell carcinoma (BSCC) and carcinosarcoma of the esophagus are rare entities, making up fewer than 2% of esophageal malignancies. Comparative genomic hybridization (CGH) in 1 case of BSCC and 2 cases of carcinosarcoma and subsequent array CGH in 1 case each of BSCC and carcinosarcoma revealed common c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPZ1O7UUUISPNR

    authors: Schaefer IM,Enders C,Polten A,Haller F,Frölich AM,Cameron S,Schüler P,Schweiger P,Gunawan B,Beham A,Füzesi L

    更新日期:2011-04-01 00:00:00

  • JAK2 mutations across a spectrum of venous thrombosis cases.

    abstract::The JAK2(V617F)mutation is recurrent in polycythemia vera and essential thrombocythemia, which are myeloproliferative neoplasms frequently associated with arterial and venous thromboembolism. It has also been reported as a marker for occult myeloproliferative disorder (MPD) in patients with splanchnic venous thrombosi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP7VO4HAIZYATP

    authors: Shetty S,Kulkarni B,Pai N,Mukundan P,Kasatkar P,Ghosh K

    更新日期:2010-07-01 00:00:00