Abstract：
OBJECTIVES:Pure erythroid leukemia (PEL) is exceptionally rare in the pediatric setting. Four pediatric PEL cases with t(1;16)(p31;q24) NFIA-CBFA2T3 were reported previously. We present a case of an infant with PEL presenting with erythroblastic sarcoma and harboring a novel t(1;8)(p31.3;q21.3) NFIA-RUNX1T1 fusion detected by RNA sequencing and conventional karyotype. METHODS:Bone marrow (BM) and abdominal mass biopsies from the patient were evaluated with extensive immunohistochemical, flow cytometric, cytogenetic, and molecular studies. RESULTS:The patient was a female infant who presented between 2 and 5 months of age with cytopenias and an enlarging abdominal mass. Blasts in the BM and abdominal mass expressed CD71 and CD117 with focal expression of CD43, E-cadherin, epithelial membrane antigen, and hemoglobin A. They were negative for additional myeloid, lymphoid, and nonhematolymphoid markers. These findings were most consistent with PEL and erythroblastic sarcoma. RNA sequencing revealed the novel NFIA-RUNX1T1 fusion. CONCLUSIONS:Along with the previously reported PELs with NFIA-CBFA2T3 fusions, we describe a subset of PELs that occur in children, that frequently display extramedullary disease, and that harbor rearrangements of NFIA with core binding factor genes. We hypothesize that, together, these cases represent a rare but distinct clinicopathologic group of pediatric PELs with recurrent genetic abnormality.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
King RL,Siaghani PJ,Wong K,Edlefsen K,Shane L,Howard MT,Reichard KK,Mai M,Viswanatha DS,Greipp PT,Goble TA,Ruiz M,Hara Hdoi
10.1093/ajcp/aqaa216subject
Has Abstractpub_date
2020-12-14 00:00:00eissn
0002-9173issn
1943-7722pii
6032724pub_type
杂志文章abstract：:Cryostat sections from fully developed papular lesions of lymphomatoid papulosis (histologic subtype A or B) have been examined by immunoenzymatic staining with 24 monoclonal antibodies against lymphoid cells and their subsets. The lesions demonstrated essentially identical cellular compositions and consisted of T-lym...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.5.587
更新日期：1985-11-01 00:00:00
abstract：:Automation was introduced into the clinical microbiology laboratory in the 1960s but initially met with limited success. Today, instruments are an integral part of many clinical laboratories and are used for microbial detection, identification, and susceptibility testing; detection of positive blood cultures; screenin...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1992-10-01 00:00:00
abstract：:The cases of 20 patients, each of whom has a positive lupus erythematosus cell preparation and a negative antinuclear factor test, are presented. The concept of a false-positive lupus erythematosus preparation is suggested. Five common mechanisms causing a false-negative antinuclear factor test are discussed and evalu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.3.495
更新日期：1976-09-01 00:00:00
abstract：:In situ hybridization with specific RNA radiolabeled probes was used to analyze the production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) by 21 low-grade follicular lymphomas (FLs). All of the 21 FLs tested contained lymphokine-synthesizing cells in the interfollicular and follicular areas. Enumeration o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.1.55
更新日期：1991-01-01 00:00:00
abstract：:Two monoclonal antibodies, UCD/AB 6.11 and UCD/PR 10.11, were evaluated for their patterns of immunohistochemical reactivity in a survey of paraffin-embedded human tissues by the avidin-biotin-immunoperoxidase technique. By two-dimensional immunoblotting, UCD/AB 6.11 reacts with keratin number 18 and UCD/PR 10.11 iden...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.4.472
更新日期：1988-04-01 00:00:00
abstract：:The involvement of Nonspecific Crossreacting Antigen (NCA) in the immunohistological demonstration of Carcino Embryonic Antigen (CEA) in 56 benign and 92 malignant lesions of the breast was analyzed. For this purpose, the authors utilized both polyclonal antisera and monoclonal antibodies. Polyclonal anti-CEA sera wer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/82.5.526
更新日期：1984-11-01 00:00:00
abstract：:The measurement of beta-thromboglobulin (BTG) and platelet lactic dehydrogenase (LDH) as markers for alpha-granule release and platelet lysis, respectively, has been reported to correlate with the amount of damage incurred during the preparation and storage of platelet concentrates. We compared the concentrations of t...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.6.719
更新日期：1983-06-01 00:00:00
abstract：:A case of septicemia caused by Pseudomonas paucimobilis is reported. This represents the first definitive case of disease produced by this yellow-pigmented, nonfermentative bacillus. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.5.882
更新日期：1979-11-01 00:00:00
abstract：:A critical limit protocol requiring that all point of care glucose meter readings > 22.2 mmol/L (400 mg/dL) and < 2.2 mmol/L (40 mg/dL) be immediately confirmed by the laboratory was assessed. A total of 193 (2%) of 9,523 glucose meter determinations (63 patients) were > 22.2 or < 2.2 mmol/L. One hundred twenty-two (6...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.3.390
更新日期：1996-09-01 00:00:00
abstract：:Chemotherapy is a well-established therapeutic approach for several malignancies, but its clinical efficacy is often limited by its related cardiotoxicity, which leads to cardiomyopathy, possibly evolving into heart failure. To detect cardiac damage, the adopted diagnostic approach is the estimation of left ventricula...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPB66LRIIVMQDR
更新日期：2008-11-01 00:00:00
abstract：:The diagnostic expert system for colonic lesions (DESCL) was designed to discriminate colonic adenoma and adenocarcinoma from normal colonic tissue. Although it was originally developed for use in conjunction with a machine vision analytic system, the DESCL has evolved into a teaching tool and a model for conceptual m...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1990-10-01 00:00:00
abstract：:A comparative study of large cell lymphoma (LCL) (ten B and ten T), Hodgkin's disease (15 cases), and true histiocytic lymphoma (two cases) was undertaken, using formalin-fixed paraffin-embedded tissue sections, a panel of eight antibodies, and one lectin to determine if any particular antibody or immunologic profile ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.591
更新日期：1986-11-01 00:00:00
abstract：:The case of a 3-week-old infant with Coxsackie B3 meningoencephalitis is discussed. Hypoglycorrhachia and neutrophils in the cerebrospinal fluid characterized the first ten days of her illness. Coxsackie B3 should be added to the list of enteroviruses that can cause hypoglycorrhachia during infections of the central n...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.6.947
更新日期：1978-12-01 00:00:00
abstract：:There are wide variations in the preparation of histologic sections from endoscopic esophageal biopsy specimens. We evaluated serial step sections from 261 esophageal or gastroesophageal junction biopsies at 4 levels to determine the first level at which goblet cell metaplasia (GCM) was detected. Deeper step sections ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/MGRX-LQG9-QVRV-VMCG
更新日期：2005-06-01 00:00:00
abstract：:Monoclonal immunoradiometric assays (IRMA) for human chorionic gonadotropin (hCG) are available for either intact hCG (IhCG) or beta subunit hCG (beta hCG). The authors evaluated the clinical applications of both methods. Serum samples (N = 180) were divided into the following five clinical groups: Group 1: elevated l...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.3.347
更新日期：1990-03-01 00:00:00
abstract：:Differential characterization of the "reticulum cell" in lymphoreticular neoplasms. Am J Clin Pathol. 64: 171-179, 1975. The term "reticulum cell" is confusing, having been applied to the cells involved in many hematopoietic neoplasms, such as reticulum-cell sarcoma, histiocytic medullary reticulosis, leukemic reticul...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.2.171
更新日期：1975-08-01 00:00:00
abstract：:Mycobacterium leprae causes leprosy. M leprae strains collected worldwide have been genetically clonal, which poorly explains the varying severity and clinical features of the disease. We discovered a new Mycobacterium species from 2 patients who died of diffuse lepromatous leprosy (DLL). The Mycobacterium was purifie...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP72FJZZRRVMM
更新日期：2008-12-01 00:00:00
abstract：Objectives:Diagnosis of B-cell acute lymphoblastic leukemia (B-ALL) requires immunophenotypic evidence of B-lineage and absence of specific myeloid or T-lineage markers. Rare cases of otherwise typical B-ALL express myeloperoxidase (MPO) detectable by flow cytometry with an absence of other myeloid markers, but the cli...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqx021
更新日期：2017-04-01 00:00:00
abstract：:Fungal organisms morphologically resembling Candida were found in one third of 72 consecutive surgically resected gastric ulcers. In over half of these cases the organisms were present in the mycelial form. Large numbers of fungi forming clusters were found in 13 cases (18%) and were associated with a surprisingly hig...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.2.137
更新日期：1979-02-01 00:00:00
abstract：:An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.3.337
更新日期：1986-03-01 00:00:00
abstract：:"Chondroid chordoma" is a controversial and confusing entity that was originally described by Heffelfinger and colleagues as a biphasic malignant neoplasm possessing elements of both chordoma and cartilaginous tissue. Because the premise for this distinction was based strictly on histomorphologic criteria, the light m...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/103.3.271
更新日期：1995-03-01 00:00:00
abstract：:One of the functions of von Willebrand factor (vWF) is to serve as a carrier of clotting factor VIII (FVIII). Deficiency of this function results in the von Willebrand disease (vWD) variant type 2N, which resembles hemophilia A. We describe a new sandwich enzyme-linked immunosorbent assay (ELISA) to study the ability ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/109.3.347
更新日期：1998-03-01 00:00:00
abstract：:We assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other B-cell non-Hodgkin lymphomas (NHLs) for 11q13 breakpoints using a 2-color fluorescence in situ hybridization (FISH) assay that uses an 11q13 probe centered on the CCND1 gene and a centromeric chromosome 11 probe (CEP11). The number of nu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/69EJ-RFM5-E976-BUTP
更新日期：2000-08-01 00:00:00
abstract：:Intrauterine pulmonary infarction is extremely rare. In a series of 775 stillborn and newborn infants, only a few cases of acute pulmonary infarction were reported. The authors present the findings from a full-term infant who died at 9 hours of age of a chronic pulmonary infarction. Several pathogenic mechanisms, incl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.6.701
更新日期：1993-12-01 00:00:00
abstract：OBJECTIVES:Although diffuse alveolar damage, a subtype of acute lung injury (ALI), is the most common microscopic pattern in coronavirus disease 2019 (COVID-19), other pathologic patterns have been described. The aim of the study was to review autopsies from COVID-19 decedents to evaluate the spectrum of pathology and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa156
更新日期：2020-11-04 00:00:00
abstract：:Platelet volume measurements have been used to differentiate consumptive and hypoplastic thrombocytopenia. Since thrombocytopenia is a frequent complication of neonatal sepsis, the authors explored the utility of correlating mean platelet volume (MVP) and platelet distribution width (PDW) with bacteremia. In a sample ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.3.391
更新日期：1990-03-01 00:00:00
abstract：:In-situ and microinvasive adenocarcinoma of the uterine cervix. A clinical, cytologic and histologic study of 14 cases. Am J Clin Pathol 64: 155-70, 1975. The clinicopathologic features of 14 cases of in-situ and microinvasive adenocarcinoma of the cervix, encountered during the period 1961-1974, form the basis of thi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.2.155
更新日期：1975-08-01 00:00:00
abstract：:To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/106.2.180
更新日期：1996-08-01 00:00:00
abstract：:Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.2.177
更新日期：1980-02-01 00:00:00
abstract：:A population-based case-control study of college students was undertaken to estimate the effect of a positive clinical test for human papillomavirus (HPV) DNA (the Virapap test) on the rate of squamous intraepithelial lesions (SIL) of the uterine cervix. When age, multiple lifetime sexual partners, and oral contracept...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.1.6
更新日期：1993-07-01 00:00:00
