Measuring direct thrombin inhibitors with routine and dedicated coagulation assays: which assay is helpful?

Abstract:

:The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatroban, and bivalirudin) in 6 pooled plasma specimens on routine coagulation assays (activated partial thromboplastin time [aPTT], prothrombin time [PT], and thrombin time [TT]) and dedicated DTI assays (Hemoclot, HemosIL, the ecarin clotting time, and a chromogenic ecarin clotting time) on 2 coagulation analyzers. We found routine tests to be nondiscriminative between concentrations of different DTIs in the aPTT. Moreover, for PT and TT, the responses for different DTIs differed. This was similar for ecarin clotting assays. The Hemoclot and HemosIL assays showed identical linear increases for all 3 DTIs. We conclude that dedicated calibrated assays based on a diluted TT (Hemoclot and HemosIL) appear to be the most suitable for monitoring purposes.

journal_name

Am J Clin Pathol

authors

Curvers J,van de Kerkhof D,Stroobants AK,van den Dool EJ,Scharnhorst V

doi

10.1309/AJCPQOD9WFPEYY0H

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

551-8

issue

4

eissn

0002-9173

issn

1943-7722

pii

138/4/551

journal_volume

138

pub_type

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