Antisperm antibody detection using concurrent cytofluorometry and indirect immunofluorescence microscopy.

Abstract:

:Anti-sperm antibodies from serum and seminal plasma were detected by concurrent flow cytometry and epifluorescence microscopy using fluorescein-conjugated antihuman immunoglobulins. Experimental conditions were designed, taking advantage of several monoclonal antisperm antibodies, to test aspects of the assay before clinical application. Perturbation of membrane integrity altered both the localization of binding and the number of sperm cells positive for bound antibodies. In specimens from selected infertility patients, 21.6% of the females and 40.8% of the males had significant levels of antisperm antibodies. Differences in the incidence of isoimmunity between female partners of antibody-positive or antibody-negative males and differences in the localization of antigens targeted by serum versus seminal plasma antibodies in men support the idea that, in some cases, immunity to sperm cells may be the result of altered sperm antigens.

journal_name

Am J Clin Pathol

authors

Sinton EB,Riemann DC,Ashton ME

doi

10.1093/ajcp/95.2.242

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

242-6

issue

2

eissn

0002-9173

issn

1943-7722

journal_volume

95

pub_type

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