Abstract：
:Hemizygous TP53 gene deletion is the most important adverse risk factor in chronic lymphocytic leukemia (CLL), but its relationship with p53 protein expression is unclear. We investigated 110 CLL cases and correlated nuclear p53 protein immunoreactivity with TP53 gene deletion status and other CLL-associated genetic risk factors. Fluorescence in situ hybridization detected hemizygous TP53 deletions in 15 cases (13.6%), whereas immunohistochemical analysis detected nuclear p53 protein expression in 14 (12.7%). All cases expressing nuclear p53 protein had hemizygous TP53 deletions. Hemizygous TP53 gene deletion and p53 protein expression were strongly correlated (P < .001). There was no association between p53 expression and del(13q), del(11q) or trisomy 12 in CLL cases. Our data indicate that nuclear p53 protein expression, detected by a widely available immunohistochemical method, is strongly associated with TP53 deletion and that p53 immunohistochemical analysis may be adopted as a rapid, robust diagnostic tool for risk stratification of CLL.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Chang H,Jiang AM,Qi CXdoi
10.1309/AJCPEPX1C7HHFELKsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
70-4issue
1eissn
0002-9173issn
1943-7722pii
133/1/70journal_volume
133pub_type
杂志文章abstract：:We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage cl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/C8T8-YNB4-G3W4-5PRF
更新日期：2003-07-01 00:00:00
abstract：:Infusible platelet membranes (IPMs) prepared from fresh or outdated human platelets have been shown to correct prolonged bleeding times in thrombocytopenic rabbits. In previous trials, IPMs did not seem to be immunogenic and lacked dose-limiting toxicity. The present study was undertaken to explore whether the platele...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/CCDV-3BEP-XXKP-BKDM
更新日期：2001-01-01 00:00:00
abstract：:This study aimed to ascertain pathologic findings of surgical follow-up excision (FUE) on patients who had radiologic finding of calcifications and lobular neoplasia (LN) on core biopsy. Breast core biopsy specimens from 2006-2011 with a diagnosis of pure classic-type LN (lobular carcinoma in situ [LCIS] and atypical ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPYG48TUTFIBMR
更新日期：2012-07-01 00:00:00
abstract：:Histiocytosis X, multicentric reticulohistiocytosis, juvenile xanthogranuloma, the "fibrous" type of dermatofibroma, dermatofibrosarcoma protuberans, and malignant fibrous histiocytoma are all characterized by dermal and/or subcutaneous infiltrates composed at least partially of cells having morphologic features sugge...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.2.162
更新日期：1981-02-01 00:00:00
abstract：:We describe the development and testing of a novel pre-sign-out quality assurance tool for case diagnoses that allows for the random review of a percentage of cases by a second pathologist before case verification and release of the final report. The tool incorporates the ability to record and report levels of diagnos...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPLN9DU9LNXSXA
更新日期：2010-06-01 00:00:00
abstract：OBJECTIVES:To assess the frequency of systemic mastocytosis (SM) in a large series of acute myeloid leukemia (AML) with t(8;21)(q22;q22). METHODS:We retrospectively characterized 40 bone marrow aspirate smears and biopsy specimens from patients with AML with t(8;21) for the presence of SM. Cases were assessed for mast...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP1Q0YSXEAHNKK
更新日期：2013-10-01 00:00:00
abstract：:Mantle cell lymphoma (MCL) is characterized by typical morphologic features and the CD5+, CD23-immunophenotype. However, some morphologically typical MCLs are CD23+. A t(11;14)(q13;q32) translocation is frequently found in MCL, leading to overexpression of cyclin D1. We studied the expression of cyclin D1 in 50 small ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/R2BC-2H7T-7KB6-0YG8
更新日期：2002-02-01 00:00:00
abstract：:Three activated partial thromboplastin time (APTT) reagent test systems, General Diagnostics Automated APTT, American Dade Actin FS, and Pacific Hemostasis (Thromboscreen KAPTT) reagent, containing different activators for the APTT assay, were evaluated for their precision and sensitivity to factor deficiencies in the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.1.43
更新日期：1986-01-01 00:00:00
abstract：:Experiences of the CAP Survey Program in reticulocyte counts and morphologic identification of reticulocytes from 1971 to 1974 are reviewed. Problems of morphologic identification are reflected in an excessive variance of reticulocyte counts. Statistical sources of variations in counting are identified. The older, ori...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:A population-based case-control study of college students was undertaken to estimate the effect of a positive clinical test for human papillomavirus (HPV) DNA (the Virapap test) on the rate of squamous intraepithelial lesions (SIL) of the uterine cervix. When age, multiple lifetime sexual partners, and oral contracept...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.1.6
更新日期：1993-07-01 00:00:00
abstract：:We studied the beta-catenin immunohistochemical profile in tumors expressing shadow cells: pilomatricoma, 10 cases; calcifying odontogenic cyst, 6 cases; and craniopharyngioma, 9 cases. There was strong membranous, cytoplasmic, and nuclear staining of the immature basaloid cells in all of these tumors. Shadow cells we...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/EALE-G7LD-6W71-67PX
更新日期：2003-11-01 00:00:00
abstract：:The authors report the advantages of a new anticoagulant-antiaggregant mixture that avoids the deleterious effects of ethylenediaminotetraacetic acid (EDTA) on mean platelet volume and also prevents EDTA-induced platelet clumping. It is suitable for routine cell counting and sizing with the Coulter Counter S-Plus STKR...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.6.760
更新日期：1990-06-01 00:00:00
abstract：:Venous thromboembolism (VTE) results from the interaction of the Virchow triad (venous stasis, endothelial injury, and hypercoagulability). Risk factors for increased hypercoagulability, or thrombophilia, include activated protein C resistance/factor V Leiden, the prothrombin G20210A mutation, deficiencies of the natu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP5SQT3ZKYQFBM
更新日期：2012-04-01 00:00:00
abstract：:A simple assay based on molecular light scattering was developed to detect soluble immune complexes. In this assay the percentage of relative light scattering (%RLS) caused by complexes in buffer containing polyethylene glycol 6000 (PEG) was measured with a laser nephelometer. In low concentrations of PEG (3%-5%), IgG...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/74.6.804
更新日期：1980-12-01 00:00:00
abstract：:A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.4.537
更新日期：1975-10-01 00:00:00
abstract：:Acute paraplegia developed in a 53-year-old man with pulmonary aspergilloma because of contiguous extension of Aspergillus infection to the epidural and subdural spaces and spinal cord. Histopathologic findings of the spinal cord showed Aspergillus hyphae penetrating the myelin sheath and myelomalacia, predominantly i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.6.763
更新日期：1985-12-01 00:00:00
abstract：:The results of a direct anaerobic susceptibility testing method were compared with those of a standardized method. Seventy-three blood culture isolates and eight antimicrobial agents were used. The results of the direct and standardized testing methods were within one two-fold dilution for 95.2% of the 584 anaerobe-an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.4.558
更新日期：1980-04-01 00:00:00
abstract：:We determined whether there were additional diagnostic findings in additional level sections performed on polyps with no pathologic diagnosis (NPD) or those in which only lymphoid aggregates (LAs) were seen initially and determined the level at which findings were identified. All colorectal biopsy specimens submitted ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/27NQ-A1R2-DQ41-20Y7
更新日期：2002-03-01 00:00:00
abstract：:Recent studies in Western populations have shown that trisomy 3 is the most frequent chromosomal abnormality in primary gastric lymphoma (PGL). To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 case...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/110.3.347
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVES:Pool testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) preserves testing resources at the risk of missing specimens through specimen dilution. METHODS:To determine whether SARS-CoV-2 specimens would be missed after 10:1 pooling, we identified 10 specimens with midrange (ie, 25-34 cycl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa273
更新日期：2021-01-05 00:00:00
abstract：:We sought to characterize how well computerized "case flags" performed in evaluating critical diagnoses in anatomic pathology. All cases identified by a pathologist at sign-out and flagged as a critical diagnosis in anatomic pathology in 2 hospital laboratories during a 3-year period were reviewed. A subset of all cri...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPL1N5KBAFFDHV
更新日期：2012-03-01 00:00:00
abstract：:Adenocarcinomas of the sigmoid colon and rectum were studied for the presence of blood group substances of the A, B, H system using the method of specific erythrocytic adherence. Of 17 adenocarcinomas studied, eight were found to have reactivity for blood group substances. All eight tumours showing reactivity for bloo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.6.594
更新日期：1978-06-01 00:00:00
abstract：:A 63-year-old white man had simultaneous onset of the Guillain-Barré syndrome and the nephrotic syndrome, three weeks after having an upper respiratory tract infection. Many granular casts were noticed in the urine sediment. Renal biopsy specimens showed early membranous glomerulonephritis and acute interstitial nephr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.4.563
更新日期：1982-10-01 00:00:00
abstract：:To our knowledge, there are no universally accepted, evidence-based guidelines for how to resolve the HER2 status of tumors demonstrating equivocal amplification. The present study was based on 17 breast core biopsy specimens demonstrating invasive carcinoma with equivocal HER2 amplification, defined as an HER2/chromo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/KFKDNK8CENVN24VU
更新日期：2008-03-01 00:00:00
abstract：:To evaluate the etiologic role of human papillomavirus (HPV) in oral carcinogenesis, DNA samples were purified from 103 oral squamous cell carcinoma (OSCC) and 30 normal oral mucosal (NOM) specimens. A nested polymerase chain reaction, DNA sequencing, and gene-chip HPV typing were used to identify multiple HPV types i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/C5P6-NUQ2-NW6L-CTBP
更新日期：2003-12-01 00:00:00
abstract：:The blood counts and lactic dehydrogenase values of eight patients with thrombotic thrombocytopenic purpura (TTP) were reviewed in relation to the clinical course. Three of the eight patients died. In these patients, the hemoglobin was significantly lower and the LDH higher at the time of presentation than that of the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.5.700
更新日期：1983-11-01 00:00:00
abstract：:A case of unilateral gonadoblastoma in association with a ruptured ectopic tubal pregnancy is presented. The patient had normal menstrual history prior to a missed period followed by abdominal pain and the passage of clots. The surgical specimens, in addition to the ruptured tubal pregnancy, showed nodular foci of typ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.1.121
更新日期：1976-01-01 00:00:00
abstract：:Sex cord tumor with annular tubules (SCTAT) is a distinctive ovarian sex cord-stromal tumor. The authors describe a case of SCTAT arising outside the ovaries, in the fallopian tube. The authors are aware of only one other case of extra-ovarian SCTAT, in an umbilical hernia sac in an adult woman. The case reported was ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/96.2.259
更新日期：1991-08-01 00:00:00
abstract：:Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.2.177
更新日期：1980-02-01 00:00:00
abstract：:An aggressive visceral form of Kaposi's sarcoma (KS) has been reported in immunosuppressed populations, most notably in patients with acquired immunodeficiency syndrome (AIDS). This presentation contrasts from the indolent skin lesions on the extremities of older males and is distinct enough in the young adult America...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.3.366
更新日期：1986-09-01 00:00:00
