Abstract:
OBJECTIVES:Pool testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) preserves testing resources at the risk of missing specimens through specimen dilution. METHODS:To determine whether SARS-CoV-2 specimens would be missed after 10:1 pooling, we identified 10 specimens with midrange (ie, 25-34 cycles) and 10 with late (ie, >34-45 cycles) crossing threshold (Ct) values and tested these both neat and after 10:1 pooling. Final test results and Ct changes were compared. RESULTS:Overall, 17 of 20 specimens that contained SARS-CoV-2 were detected after 10:1 pooling with the Xpert Xpress SARS-CoV-2 Assay (Cepheid), rendering an 85% positive percentage of agreement. All 10 of 10 specimens with an undiluted Ct in the mid-Ct range were detected after 10:1 pooling, in contrast to 7 of 10 with an undiluted Ct in the late-Ct range. The overall Ct difference between the neat testing and the 10:1 pool was 2.9 cycles for the N2 gene target and 3 cycles for the E gene target. The N2 gene reaction was more sensitive than the E gene reaction, detecting 16 of 20 positive specimens after 10:1 pooling compared with 9 of 20 specimens. CONCLUSIONS:An 85% positive percentage of agreement was achieved, with only specimens with low viral loads being missed following 10:1 pooling. The average impact on both reverse transcription polymerase chain reactions within this assay was about 3 cycles.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Procop GW,Tuohy M,Ramsey C,Rhoads DD,Rubin BP,Figler Rdoi
10.1093/ajcp/aqaa273subject
Has Abstractpub_date
2021-01-05 00:00:00eissn
0002-9173issn
1943-7722pii
6063387pub_type
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
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更新日期:1994-01-01 00:00:00
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