Abstract:
:Micropapillary lung adenocarcinoma (MPA) has been reported as an aggressive variant of adenocarcinoma, frequently manifesting at high stage with a poor prognosis. We analyzed the clinical and molecular profile of 15 primary MPAs for K-ras, EGFR, and BRAF mutations and performed fluorescence in situ hybridization for EGFR amplification. In our study, 11 (73%) of 15 MPAs harbored mutually exclusive mutations: 5 (33%) K-ras, 3 (20%) EGFR, and 3 (20%) BRAF. Mutations in all 3 genes occurred in patients with a smoking history and tumors with mucinous differentiation and secondary lepidic, acinar, and solid growth, suggesting that in a Western population, cytomorphologic correlation with genetic mutations is more unpredictable than in Japanese cohorts. We conclude that K-ras, EGFR, and BRAF mutations are disproportionately seen in adenocarcinomas of lung with a dominant micropapillary growth pattern compared with conventional adenocarcinoma in our institutional experience.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
De Oliveira Duarte Achcar R,Nikiforova MN,Yousem SAdoi
10.1309/AJCPBS85VJEOBPDOsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
694-700issue
5eissn
0002-9173issn
1943-7722pii
131/5/694journal_volume
131pub_type
杂志文章abstract::Immunohistochemical techniques applied to fresh frozen sections of metastatic malignant melanoma tissue revealed abundant fibrinogen (or fibrin I) in perivascular areas throughout the tumor connective tissue stroma. Fibrin was readily detected in a focal distribution in the connective tissue around nodules of viable t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/93.4.516
更新日期:1990-04-01 00:00:00
abstract:OBJECTIVES:Sclerosing pneumocytomas are rare pulmonary neoplasms that are typically benign. However, rare patients experience progressive disease, and therapy targeting specific genetic underpinnings could be an attractive therapeutic option. Recent studies have found recurrent AKT 1 mutations in sclerosing pneumocytom...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa136
更新日期:2020-11-04 00:00:00
abstract::The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPAH5WR2LFTUSI
更新日期:2011-12-01 00:00:00
abstract::Diffuse alveolar damage (DAD) is usually considered a generalized lung process. During five years the authors observed 83 patients with generalized DAD in 827 adult autopsies (10.1%) and 10 patients with identical, but localized, lesions. The authors propose the term regional alveolar damage (RAD) to designate localiz...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/92.1.10
更新日期:1989-07-01 00:00:00
abstract::The lupus anticoagulant was identified in three patients. Laboratory studies gave evidence of inhibitory activity directed against phospholipid and prekallikrein. Inhibition of prekallikrein has not been reported previously. When exposed to kaolin, all three patients' plasmas failed to develop the level of fibrinolyti...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.3.275
更新日期:1982-03-01 00:00:00
abstract::The pattern of spurious red blood cell parameters caused by serum cold agglutinins has been well characterized for the Coulter counters but not the Ortho ELT-8. The pattern consists of a spurious increase in mean corpuscular volume (MCV), a discrepancy between the hemoglobin and the hematocrit, the latter falsely redu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.2.218
更新日期:1985-02-01 00:00:00
abstract::Adenovirus was cultured from various gastrointestinal sites and one lung specimen from seven immunosuppressed patients, four of whom were bone marrow transplant recipients. Histologic examination and in situ hybridization studies of the lung demonstrated adenovirus in the bronchial epithelium and alveolar lining cells...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.4.555
更新日期:1992-04-01 00:00:00
abstract::The visible absorption spectra of dilutions of commercially prepared chemistry control sera closely mimic those of jaundiced amniotic fluids in cases of fetal rhesus isoimmunization. The authors have assessed the use of these materials for the quality control of net absorbance measurements at 450 nm on amniotic fluids...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.4.513
更新日期:1985-10-01 00:00:00
abstract::Reports of sex steroid receptor expression in chordoma suggest that these tumors may be responsive to hormone manipulation therapy. Immunohistochemical stains for estrogen receptor (ER)-alpha, ER-beta, progesterone receptor (PR), androgen receptor (AR), and cyclooxygenase 2 (COX-2), were performed on a tissue microarr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/8T2NPHLK5X5WQ3E7
更新日期:2007-09-01 00:00:00
abstract::Data from 2.5 million glucose analyses on pools of lyophilized human quality control serum were used to evaluate analyte stability in the prereconstitution phase. Input information was from laboratories in Regional Quality Control Programs that use CAP Quality Assurance Service (QAS) data processing. Of 31 pools in us...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is characterized by typical morphologic features and the CD5+, CD23-immunophenotype. However, some morphologically typical MCLs are CD23+. A t(11;14)(q13;q32) translocation is frequently found in MCL, leading to overexpression of cyclin D1. We studied the expression of cyclin D1 in 50 small ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/R2BC-2H7T-7KB6-0YG8
更新日期:2002-02-01 00:00:00
abstract::In this study, I have estimated the percentage of tumor by visual estimate in 447 prostatectomy specimens from American Veterans Affairs patients and related this measurement to overall survival. Although percentage of tumor was significantly related to the serum prostate-specific antigen level, tumor stage, and Gleas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPX5MAMNMFE6FQ
更新日期:2009-01-01 00:00:00
abstract::Urine cultures represent 40-50% of specimens processed by hospital clinical microbiology laboratories. An accurate rapid screening method to detect positive urine cultures could greatly improve laboratory efficiency. Three hundred eighty-two unselected urines were screened by nephelometry at 2, 4, and 5 hours of shaki...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/77.3.305
更新日期:1982-03-01 00:00:00
abstract::The reactivity of eight preferential B-cell (L26, 4KB5, and KiB3) and T-cell (polyclonal CD3, Leu22, MT-1, UCHL-1, and OPD4) antibodies which detect paraffin-resistant antigens was examined by a three-step immunoperoxidase technique in 111 formalin-fixed, paraffin-embedded diffuse aggressive non-Hodgkin's lymphomas (N...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.3.284
更新日期:1994-09-01 00:00:00
abstract::Cytologic features of the cell-stroma interface are useful in distinguishing between monomorphic adenomas of the basal cell type and adenoid cystic carcinoma. In basal cell adenomas, the collagenous stroma interdigitates with adjacent cells, whereas in adenoid cystic carcinoma, the two are separated by a sharp smooth ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/106.1.35
更新日期:1996-07-01 00:00:00
abstract:Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw212
更新日期:2017-03-01 00:00:00
abstract::Noncardiogenic pulmonary edema caused by transfusion has been observed for almost 60 years. Today, we know this entity as transfusion-related acute lung injury (TRALI). TRALI is an uncommon but potentially fatal adverse reaction to transfusion of plasma-containing blood components. It is typified by dyspnea, cough, hy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/D3F7BXH466AE3G0P
更新日期:2008-02-01 00:00:00
abstract::The Travenol Hem-O-Scan and the Instrumentation Laboratory (IL) system for determination of the oxygen dissociation curve and P50 values have been evaluated. Using the Hem-O-Scan, an oxygen dissociation curve and P50 value may be obtained on 2 microliter of blood in 40 min, though the pH is not measured. In the IL sys...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.3.395
更新日期:1981-03-01 00:00:00
abstract:OBJECTIVES:We describe the morphologic and immunohistochemical features of 17 endometrial stromal neoplasms, 16 sarcomas, and one stromal nodule. METHODS:We reviewed 35 cases interpreted as endometrial stromal neoplasms, but 17 high-grade endometrial stromal sarcomas (ESS) and one case of mixed endometrial sarcoma and...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPS88CMJRXZBWA
更新日期:2014-06-01 00:00:00
abstract::Hepatocytes with orcein-positive ground-glass cytoplasm have been shown to contain abundant hepatitis B surface antigen in the cisternae of excess smooth endoplasmic reticulum. Hepatocytes with similar cytoplasmic changes that did not react with orcein were observed in 26.5% of 49 cases of cirrhosis. These cells exhib...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.4.498
更新日期:1981-04-01 00:00:00
abstract::We assessed the Sysmex UF-50 for reproducibility of results and carryover rate by performing between- and within-run precision analyses on 315 urine samples, evaluated the feasibility of using the UF-50 to measure urinary cellular and noncellular components by comparing results from the UF-50 with results of manual ur...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/RT7X-EMGF-G8AV-TGJ8
更新日期:2001-04-01 00:00:00
abstract::Several adulterants are used to mask tests for abused drugs in urine. Adulterants such as "Klear" and "Whizzies" contain potassium nitrite, and "Urine Luck" contains pyridinium chlorochromate (PCC). The presence of these adulterants cannot be detected by routine specimen integrity checks (pH, specific gravity, and tem...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/9Q2G-6CTH-XT16-HCCC
更新日期:2002-02-01 00:00:00
abstract::Since the introduction of the triple screen, the emergence of additional analytes found to be associated with trisomy 21 pregnancies has challenged the clinical pathology and obstetric communities to determine which combination provides the best performance characteristics as a screening test. The cost, practicality, ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/B7DBBLJW0W8P9G06
更新日期:2003-12-01 00:00:00
abstract::Three stillborn fetuses are reported in which an abdominal wall defect was associated with defects in the urogenital and anal region. Autopsy of these fetuses provided clues indicative of how and where the embryonic development went wrong. The malformation involved a disturbance of the cell deposition process, occurri...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.5.640
更新日期:1991-11-01 00:00:00
abstract::Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.1.108
更新日期:1992-01-01 00:00:00
abstract::Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors characterized by mutations of KIT or PDGFRA. The objectives of this study were to evaluate BRAF mutations in GISTs and then to correlate BRAF mutational status in the tumor with clinical parameters, with B-raf expression, and with activation of some cellul...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPPCKGA2QGBJ1R
更新日期:2010-01-01 00:00:00
abstract::A case of a patient with otherwise typical multiple myeloma whose myeloma cells were phagocytic is described. Phagocytosis of erythrocytes, erythrocytic precursors, lymphocytes, and platelets was found. Ingestion of latex particles by myeloma cells was demonstrated in vitro. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/71.6.722
更新日期:1979-06-01 00:00:00
abstract::Non-Hodgkin lymphoma (NHL) is more likely to develop in patients with Hodgkin disease (HD) than in the general population. Although reports of synchronous or metachronous HD and NHL are not uncommon in the literature, the biologic relationship of these 2 malignant neoplasms often is unclear. A larger-than-expected fra...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/W1GW-43HT-793U-F86R
更新日期:2004-05-01 00:00:00
abstract::A patient with acquired von Willebrand disease associated with multiple myeloma (IgG-lambda) is described. Mixture of his plasma or IgG fraction with washed control platelets resulted in the inhibition of aggregation with ristocetin, but mixture of control plasma or IgG fraction with washed patient platelets showed no...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.5.663
更新日期:1987-05-01 00:00:00
abstract::A case of unilateral gonadoblastoma in association with a ruptured ectopic tubal pregnancy is presented. The patient had normal menstrual history prior to a missed period followed by abdominal pain and the passage of clots. The surgical specimens, in addition to the ruptured tubal pregnancy, showed nodular foci of typ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.1.121
更新日期:1976-01-01 00:00:00