Abstract：
:Immunohistochemical techniques applied to fresh frozen sections of metastatic malignant melanoma tissue revealed abundant fibrinogen (or fibrin I) in perivascular areas throughout the tumor connective tissue stroma. Fibrin was readily detected in a focal distribution in the connective tissue around nodules of viable tumor. Staining for D-dimer of cross-linked fibrin (using an antibody that cross-reacted with fragment D of fibrinogen) coincided with staining for fibrin. Diffuse staining of tumor cell bodies was observed for Factor X, and Factor XIII ("a" subunit) was detected in scattered areas of connective tissue throughout the tumors. Factor VII was not detected, and only rare tumor cells stained for tissue factor. These results support the concept that a tumor cell-associated, thrombin-generating pathway exists in situ in malignant melanoma tissue that includes Factor X but neither tissue factor nor Factor VII. By contrast, tumor cell staining was observed rarely for urokinase and to a variable extent for tissue plasminogen activator.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Wojtukiewicz MZ,Zacharski LR,Memoli VA,Kisiel W,Kudryk BJ,Rousseau SM,Stump DCdoi
10.1093/ajcp/93.4.516subject
Has Abstractpub_date
1990-04-01 00:00:00pages
516-21issue
4eissn
0002-9173issn
1943-7722journal_volume
93pub_type
杂志文章abstract：OBJECTIVES:Lymphoid enhancer binding factor 1 (LEF1) is expressed in most cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and has shown utility in distinguishing CLL/SLL from other small B-cell lymphomas. LEF1 expression has not been systematically studied in CD5-positive marginal zone lympho...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz207
更新日期：2020-04-15 00:00:00
abstract：:Pleomorphic adenoma (or benign mixed tumor) of the breast is a rare benign neoplasm that might be misinterpreted both clinically and pathologically as a malignant tumor. The authors present an additional case of this unusual lesion studied by immunohistochemistry, electron microscopy, and flow cytometry. A 77-year-old...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/93.6.795
更新日期：1990-06-01 00:00:00
abstract：OBJECTIVES:Pool testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) preserves testing resources at the risk of missing specimens through specimen dilution. METHODS:To determine whether SARS-CoV-2 specimens would be missed after 10:1 pooling, we identified 10 specimens with midrange (ie, 25-34 cycl...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa273
更新日期：2021-01-05 00:00:00
abstract：:Current methods for testing stool samples for hemoglobin utilize peroxidase oxidation of chemical indicators such as guaiac or benzidine. These tests have frequent false-positive and false-negative results, complicating random screening for occult gastrointestinal bleeding. The authors have developed an immunochemical...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.1.342
更新日期：1978-03-01 00:00:00
abstract：:In the differential diagnosis of Spitz nevus vs malignant melanoma, patient age provides a critical piece of clinical information, because Spitz nevi occur mostly in children and melanomas occur mostly in adults. Nevertheless, there is overlap in the age distributions of Spitz nevus and melanoma. The issue to consider...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/E14C-J6KR-D092-DP3M
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVES:Endocervical curettage (ECC) specimens may be limited by scant tissue. We evaluated whether a cellular concentration processing method could improve their diagnostic quality. METHODS:Between October 2018 and June 2019, ECC specimens were assigned chronologically to one of two groups: nonconcentrated ECC (NE...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqaa125
更新日期：2021-01-04 00:00:00
abstract：OBJECTIVES:To develop reference ranges for platelet aggregation using the Multiplate analyzer (Roche Diagnostics, Mannheim, Germany) in blood anticoagulated with sodium citrate (Na-citrate), lithium heparin (Li-heparin), or hirudin. METHODS:The study was performed at three sites on consented, healthy adults (n = 193) ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP43SEYCBJLHJ
更新日期：2014-11-01 00:00:00
abstract：Objectives:Free light chain (FLC) measurement gained a lot of interest for diagnostic workup of monoclonal gammopathy. Methods:We evaluated the performance of turbidimetric polyclonal Freelite (The Binding Site, Birmingham, UK) assays on Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland) and nephelometric monoclona...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqx037
更新日期：2017-06-01 00:00:00
abstract：:CD45/side scatter (SS) gating is widely used for isolating blasts by flow cytometry (FC). However, other cells contaminate the "blast gate" (BG); CD45/SS gating is thus imprecise, particularly when there are few blasts. We studied the BG contents in 21 myelodysplastic syndromes (MDSs), 14 myeloproliferative neoplasms ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPN4G1IZPABRLH
更新日期：2012-05-01 00:00:00
abstract：:Thirty-five consecutive renal biopsy specimens were evaluated by light histology using paraffin and glycol methacrylate embedding. The clarity of the renal structures was found to be much better, and the renal lesions better defined, using glycol methyacrylate embedding. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.5.655
更新日期：1975-11-01 00:00:00
abstract：:Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/97.1.108
更新日期：1992-01-01 00:00:00
abstract：:Four cases of acute myelofibrosis are contrasted to three cases of acute megakaryocytic leukemia. The cases were clinically indistinguishable. Light microscopic examination of hematoxylin and eosin-stained bone marrow biopsy specimens from the cases of acute myelofibrosis showed a trilinear proliferation of mature and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/88.5.578
更新日期：1987-11-01 00:00:00
abstract：OBJECTIVES:We report a case of a patient with diabetes mellitus and unexpectedly low hemoglobin A1c results associated with paroxysmal nocturnal hemoglobinuria (PNH). We review the impact of shortened RBC half-life on the interpretation of hemoglobin A1c levels. METHODS:Patient history and laboratory test results were...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPTK1HPR2KULJU
更新日期：2014-08-01 00:00:00
abstract：:The present immunoperoxidase study attempted to localize aldosterone and cortisol in formalin-fixed paraffin-embedded adrenal glands or tumors. Apparently, steroid substances are solubilized during routine tissue processing, and the cytoplasm of adrenal cortical cells is not stained by either antialdosterone or antico...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.1.92
更新日期：1981-01-01 00:00:00
abstract：OBJECTIVES:We describe the morphologic and immunohistochemical features of 17 endometrial stromal neoplasms, 16 sarcomas, and one stromal nodule. METHODS:We reviewed 35 cases interpreted as endometrial stromal neoplasms, but 17 high-grade endometrial stromal sarcomas (ESS) and one case of mixed endometrial sarcoma and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPS88CMJRXZBWA
更新日期：2014-06-01 00:00:00
abstract：:A study was made of quantitative urine cultures in bacteremic patients with urinary tract infection as the only identifiable source. These patients represent the most serious form of urinary tract infection and should be recognized as significant by the criteria used for interpreting quantitative urine cultures. In 83...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.5.616
更新日期：1986-05-01 00:00:00
abstract：:Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/7Y7N-M1WM-R0YK-M2VA
更新日期：2002-05-01 00:00:00
abstract：:Although Stat tests are one of the most important features of clinical laboratory performance, the indexed literature is devoid of significant discussion of the subject. The authors, undertook a study by querying hospital laboratories about their current practices and received 38 useable answers dealing with the types...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/69.5.520
更新日期：1978-05-01 00:00:00
abstract：:Seven monoclonal antibodies of rat origin were generated against human epidermal keratins and were assayed by immunoblot and by immunoperoxidase staining of frozen sections from various human epithelial tissues. By immunoblotting, the antibodies recognized two different subsets of high molecular weight cytokeratin pol...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/89.3.315
更新日期：1988-03-01 00:00:00
abstract：:Mycobacteriology results of the CAP proficiency testing programs are reviewed for the years 1975-1981. Survey participants have shown a continuing and improving ability to isolate and identify Mycobacterium tuberculosis, nontuberculous mycobacteria, and to identify acid-fast bacteria in stain preparations. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:A 26-year-old woman who had undergone orthotopic heart transplantation because of dilative cardiomyopathy received a triple-drug immunosuppressive regimen (cyclosporine A, azathioprine, and prednisolone). During her relatively frequent episodes of acute rejection, she was treated with methylprednisolone and repeated a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.1.95
更新日期：1990-07-01 00:00:00
abstract：:A patient with an acute burn was noticed to have spuriously elevated platelet counts on an automated system. The major factor accounting for this discrepancy was the presence of numerous microspherocytes which were in the same size range of platelets. It is thus important to add microspherocytosis as an additional cau...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/77.2.220
更新日期：1982-02-01 00:00:00
abstract：OBJECTIVES:We evaluated efficacy of two dual immunohistochemistry (IHC) staining assays in assessing hairy cell leukemia (HCL) involvement in core biopsies and compared the results with concurrently collected flow cytometric data. METHODS:Overall, 148 patients with HCL (123 male, 25 female; mean age: 59.8 years; range...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz171
更新日期：2020-02-08 00:00:00
abstract：:The characteristics of the normal adult thymus in both sexes were determined in 50 cases of accidental death by a simplified quantitative histologic technic. A table of normal values derived from these findings was used for making comparisons with thymus from autopsies of 50 additional patients suffering terminal illn...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.5.657
更新日期：1981-11-01 00:00:00
abstract：:A population-based case-control study of college students was undertaken to estimate the effect of a positive clinical test for human papillomavirus (HPV) DNA (the Virapap test) on the rate of squamous intraepithelial lesions (SIL) of the uterine cervix. When age, multiple lifetime sexual partners, and oral contracept...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.1.6
更新日期：1993-07-01 00:00:00
abstract：:Infusible platelet membranes (IPMs) prepared from fresh or outdated human platelets have been shown to correct prolonged bleeding times in thrombocytopenic rabbits. In previous trials, IPMs did not seem to be immunogenic and lacked dose-limiting toxicity. The present study was undertaken to explore whether the platele...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/CCDV-3BEP-XXKP-BKDM
更新日期：2001-01-01 00:00:00
abstract：:The effect of changes in cytology laboratory costs, including the costs of new technologies, on the cost-effectiveness of cervical cancer prevention has not been studied. Using University of Iowa laboratory detection rates and costs, a decision model determined the cost-effectiveness of the laboratory with and without...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.2.259
更新日期：1999-02-01 00:00:00
abstract：:A number of genetic abnormalities have been detected in multiple myeloma (MM) using cytogenetic techniques. The prominent abnormalities are deletions of 13q and translocations affecting the IgH locus on 14q32. The recurrence of chromosomal abnormalities in MM suggests a specific role for them concerning its pathogenes...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/5KWK-P6UK-GNXX-HMYH
更新日期：2004-12-01 00:00:00
abstract：:Forty-three laboratories participated in an interlaboratory testing program offered by the College of American Pathologists for the radioimmunoassay (RIA) of carcinoembryonic antigen (CEA). Thirty correctly reported a sample with 1.9 ng of endogenous CEA per ml as less than 2.5 ng. For samples with added exogenous CEA...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:The diagnosis of myeloid leukemia cutis can be difficult, particularly in the context of an initial skin biopsy with a malignant hematopoietic neoplasm. We studied the immunohistochemical characteristics of 33 cases of myeloid leukemia cutis diagnosed at Stanford University Medical Center, Stanford, CA, 1996-2007, and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP6GR8BDEXPKHR
更新日期：2009-07-01 00:00:00
