Abstract:
OBJECTIVES:We evaluated efficacy of two dual immunohistochemistry (IHC) staining assays in assessing hairy cell leukemia (HCL) involvement in core biopsies and compared the results with concurrently collected flow cytometric data. METHODS:Overall, 148 patients with HCL (123 male, 25 female; mean age: 59.8 years; range: 25-81 years) had multiparameter flow cytometry performed using CD19, CD20, CD22, CD11c, CD25, CD103, CD123, surface light chains, CD5, and CD23. In parallel, bone marrow IHC was done using PAX5/CD103 and PAX5/tartrate-resistant alkaline phosphatase (TRAP) dual IHC stains. RESULTS:Overall sensitivity of dual IHC stains was 81.4%, positive predictive value was 100%, and negative predictive value was 81.7%. All IHC-positive cases concurred with flow cytometry data, even when HCL burden was extremely low in the flow cytometry specimens (as low as 0.02% of all lymphoid cells). CONCLUSIONS:Dual IHC stain is a sensitive tool in detecting HCL, even in cases with minimal disease involvement.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Gupta GK,Sun X,Yuan CM,Stetler-Stevenson M,Kreitman RJ,Maric Idoi
10.1093/ajcp/aqz171subject
Has Abstractpub_date
2020-02-08 00:00:00pages
322-327issue
3eissn
0002-9173issn
1943-7722pii
5607847journal_volume
153pub_type
杂志文章abstract::One of the functions of von Willebrand factor (vWF) is to serve as a carrier of clotting factor VIII (FVIII). Deficiency of this function results in the von Willebrand disease (vWD) variant type 2N, which resembles hemophilia A. We describe a new sandwich enzyme-linked immunosorbent assay (ELISA) to study the ability ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/109.3.347
更新日期:1998-03-01 00:00:00
abstract::Three policies for decreasing unnecessary cerebrospinal fluid (CSF) management Venereal Disease Research Laboratory (VDRL) tests were compared. The first policy attempted to educate physicians about the use of serologic tests for diagnosing neurosyphilis but allowed the CSF VDRL to be performed either as a screening t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.3.397
更新日期:1991-03-01 00:00:00
abstract::This review summarizes our experience using blinded review as a method to measure quality in surgical pathology. It details the specifics about how the review is performed, the weaknesses in the method, and then summarizes our results so far. These results suggest that error rates correlate with the individual patholo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/5KF89P63F4F6EUHB
更新日期:2007-01-01 00:00:00
abstract::A case of metastatic squamous cell carcinoma to bone was studied using immunoperoxidase methods for Factor VIII-related antigen, producing positive staining in the tumor cells. Electron microscopy proved the squamous nature of the tumor, and, subsequently, a pulmonary primary tumor was found. Testing of additional tum...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.1.117
更新日期:1984-01-01 00:00:00
abstract::To assess their applied clinical utility, viral cultures and serological tests for cytomegalovirus (CMV) were reviewed at Duke University Medical Center (DUMC), a 1,125-bed tertiary-care hospital. Less than 1% (3 of 1,216) of CMV cultures were positive, and 8% of serum samples (45 of 587) were positive by single sera ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.2.180
更新日期:1996-08-01 00:00:00
abstract::A patient with prolymphocytic leukemia was immunophenotyped by whole blood and Ficoll-Hypaque methods. Discordance was noted between the two methods, especially for the HLE1, B1, I2, and T1B monoclonal markers. The disparity in results reflected the use of 15 X 10(6) cells versus 1 X 10(6) cells per reaction tube. Thi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.2.227
更新日期:1989-02-01 00:00:00
abstract:Objectives:Early and accurate diagnosis remains crucial in the therapeutic management of invasive central nervous system fungal infections. Different molds have intrinsic resistance to antifungal agents; thus, morphologic differentiation is helpful to clinicians. Methods:Using three examples, we present a guide on how...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/aqx144
更新日期:2018-01-29 00:00:00
abstract:OBJECTIVES:This session of the 2013 Society of Hematopathology/European Association for Haematopathology Workshop was dedicated to B-acute lymphoblastic leukemia (B-ALL)/lymphoblastic lymphoma (LBL) with recurrent translocations and not otherwise specified. METHODS:In this review, we summarize the cases discussed duri...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCPAN7BH5DNYWZB
更新日期:2015-09-01 00:00:00
abstract::A population-based case-control study of college students was undertaken to estimate the effect of a positive clinical test for human papillomavirus (HPV) DNA (the Virapap test) on the rate of squamous intraepithelial lesions (SIL) of the uterine cervix. When age, multiple lifetime sexual partners, and oral contracept...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/100.1.6
更新日期:1993-07-01 00:00:00
abstract:OBJECTIVES:Blood component waste is an important issue at all hospitals. As an initiative of the patient blood management program at a regional health care system, the causes and extent of blood product wastage were identified, and targeted interventions to effect a reduction were implemented. METHODS:Multiple low-cos...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP42WMHSSTPHXI
更新日期:2015-03-01 00:00:00
abstract::An unusual case of acute myelomonocytic leukemia (AMML) in an 81-year-old man is described. Ultrastructural examination revealed the presence of distinct Auer bodies, which commonly are found in acute promyelocytic leukemia (APL). The Auer bodies had a splinterlike appearance with a distinct tubular substructure. The ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.2.216
更新日期:1983-08-01 00:00:00
abstract::This study compared the ability to diagnose and accurately classify hepatic mass lesions by ultrasound-guided biopsy using two different needle types: a 20 gauge needle that yielded primarily cytologic material and an 18 gauge automated core biopsy needle. Slides prepared from each of the needle types were separately ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.5.583
更新日期:1995-11-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH), using repetitive alpha satellite probes to chromosomes 9 and 17, was performed on 27 paraffinembedded bladder specimens from 18 Egyptian patients affected with bilharzial carcinoma. Fluorescence in situ hybridization results in the carcinomas and benign mucosa of patients wit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.2.234
更新日期:1996-08-01 00:00:00
abstract::We evaluated the immunohistochemical profile and specificity of CD138 reactivity in 238 specimens from hematopoietic and nonhematopoietic neoplasms. In 91 bone marrow biopsies, CD138 reactivity was observed for nonneoplastic plasma cells, neoplastic plasma cells in multiple myeloma cases (43/43), and the plasmacytic c...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/617D-WB5G-NFWX-HW4L
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVES:Superficial pleomorphic liposarcoma (PL) has a favorable prognosis compared to deeply seated PL. Given developments in the classification of lipomatous neoplasms, we reappraised a series of cases. METHODS:Retrospective clinicopathologic evaluation and genome-wide single-nucleotide polymorphism (SNP) microar...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa045
更新日期:2020-08-05 00:00:00
abstract::Immunohistochemical analysis, the most efficient way of assaying for HER-2/neu overexpression in patients with invasive breast cancer, is subject to variation in sensitivity and evaluation when used in multiple laboratories. Cell lines with differing but constant levels of HER-2/neu expression have been advocated as s...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/61AN-J1XV-3MW8-78YP
更新日期:2004-07-01 00:00:00
abstract::Seventeen tubular carcinomas occurred among 211 consecutive mastectomies (7.6%). The relative frequencies of multicentric involvement in the ipsilateral breast (56%), history of bilateral mammary cancer (38%) (P less than .01), and family history of mammary cancer in a first-degree relative (40%) (P less than .05) wer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.1.25
更新日期:1980-01-01 00:00:00
abstract::Blood gas measurements performed at the central laboratory and at a satellite laboratory of Brigham and Women's Hospital were studied to determine the differences in quality, turnaround time (TAT), and cost. The quality in both laboratories, as determined by results on proficiency and quality control samples, satisfac...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.1.7
更新日期:1994-07-01 00:00:00
abstract::Timely communication of significant or unexpected findings in surgical pathology can significantly improve patient care. Although surgical pathology critical values have been published, no systematic assessment in pediatric surgical pathology has been published. We surveyed pediatric pathologists and pediatric subspec...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/Y5K829U67GVYDWTU
更新日期:2007-12-01 00:00:00
abstract::A number of variables thought to affect measurement of prothrombin time (PT) and partial thromboplastin time (PTT) were examined in an effort to determine more precisely their effects on these measurements. On the basis of these studies, it is proposed that blood specimens be anticoagulated with one part 3.8% (w./v.) ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.5.591
更新日期:1975-11-01 00:00:00
abstract::Three anti-Factor-VIII antibodies from hemophiliacs were reacted with samples of batches of Maws commerical bovine and porcine Factor VIII concentrates manufactured over a 12-year period. The apparent antibody concentrations varied widely with the different batches of concentrates. The variations are probably due to i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.6.957
更新日期:1976-06-01 00:00:00
abstract::We compared the morphologic findings of different types of marginal zone B-cell lymphoma (MZL) involving the bone marrow (BM), including 18 splenic (SMZL), 6 extranodal (mucosa-associated lymphoid tissue lymphoma), and 6 nodal cases. The median percentage of BM involvement was 15%, and multiple overlapping patterns of...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/HRHQFBFTR8B4LXT4
更新日期:2008-05-01 00:00:00
abstract::Tumor cells in the cerebrospinal fluid of an American child with Burkitt's lymphoma are described. The morphology of the cells was demonstrated better by use of the "millipore" technic and hematoxylin and eosin staining than by the Wright-stained smear. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.2.238
更新日期:1975-08-01 00:00:00
abstract::Determination of breast cancer estrogen receptor (ER) status as a predictor of tumor response to adjuvant endocrine therapy remains a mainstay of breast cancer management. Recent second generation anti-ER antibodies and new epitope retrieval methods have produced paraffin-based immunohistochemical results that correla...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.3.332
更新日期:1996-09-01 00:00:00
abstract::Quantitative morphometry was used to evaluate the homogeneity of lymphoid cell populations in fine-needle aspiration cytology smears of lymph nodes from eight non-Hodgkin's lymphomas and four benign lymphoid proliferations. Using the standard deviation of log-transformed nuclear areas as the measure of lymphoid cell h...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/92.4.458
更新日期:1989-10-01 00:00:00
abstract::Immunoperoxidase technics were used to examine the immunoglobulin content of sections of pulmonary tissue from two typical cases of lymphomatoid granulomatosis and two cases of pneumonic processes initially diagnosed as lymphomatoid granulomatosis but representing different processes on review. Both "typical" cases an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.1.41
更新日期:1980-01-01 00:00:00
abstract::The ongoing collaborative relationship between a pathology department and the vendor of its laboratory information system is vitally important because of the strategic importance of the laboratory information system in managing the information product of the department. Laboratory information system vendors are value-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.3.363
更新日期:1992-03-01 00:00:00
abstract::The role of fine-needle aspiration (FNA) in the diagnosis of breast carcinoma is established. We evaluated whether the degree of cellular dyscohesion and the nuclear grade in FNA material of breast carcinomas are reliable prognostic predictors for ipsilateral axillary lymph node metastasis. FNA specimens from 98 women...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
abstract::Two radioimmunoassay procedures (RIA-1 and RIA-2) were evaluated for the quantitation of prostatic acid phosphatase in serum and compared with the enzymatic method and counter immunoelectrophoresis method for their specificity and sensitivity. Sera from 168 patients were analyzed and these included: normals, 27; untre...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.4.430
更新日期:1981-10-01 00:00:00
abstract::The performance characteristics of the XE-2100 (Sysmex, Kobe, Japan) automated immature granulocyte (IG) count were studied. The automated IG count was compared with the manual morphology count and with a proposed reference flow cytometric count. The comparison data were analyzed by both least-squares and Passing-Babl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/TVGKD5TVB7W9HHC7
更新日期:2007-09-01 00:00:00