Abstract:
:Immunohistochemical analysis, the most efficient way of assaying for HER-2/neu overexpression in patients with invasive breast cancer, is subject to variation in sensitivity and evaluation when used in multiple laboratories. Cell lines with differing but constant levels of HER-2/neu expression have been advocated as standard material against which assay sensitivity can be gauged. Automated image analysis could provide more precise linear measurements of HER-2/neu expression than the subjective and categorical scoring system originally designed for the HER-2/neu clinical trials. Multiple European laboratories (range, 92-126) stained 7 cell line standards on 6 successive occasions using a variety of immunohistochemical assays for HER-2/neu. During the 2-year study period, a trend toward a standard sensitivity level was observed, with significant improvement in numbers of laboratories achieving appropriate results. Image analysis gave reproducible and significantly different linear values for a total of 621 HER-2/neu results on cell lines previously categorized manually as 3+, 2+, 1+, or 0. The use of cell line standards and image analysis have the potential to assist in standardizing immunohistochemical results for predictive markers and provide more accurate and quantifiable cut points for predicting clinical response to therapy, respectively.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Rhodes A,Borthwick D,Sykes R,Al-Sam S,Paradiso Adoi
10.1309/61AN-J1XV-3MW8-78YPsubject
Has Abstractpub_date
2004-07-01 00:00:00pages
51-60issue
1eissn
0002-9173issn
1943-7722journal_volume
122pub_type
杂志文章abstract::New federal regulations require review of all available negative gynecologic smears within the previous 5 years for each patient with a current high grade squamous intraepithelial lesion (HGSIL) or above. Two university hospitals retrospectively reviewed all patients who had HGSIL or above and who had prior negative s...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/101.1.19
更新日期:1994-01-01 00:00:00
abstract::This investigation was designed to improve reference information and to evaluate the influences of sample distribution and age on the derived reference intervals. Specimens from 127 men were collected after a 12- to 14-hour fast and analyzed by 60 different laboratory procedures. Differences in the reference intervals...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/79.5.574
更新日期:1983-05-01 00:00:00
abstract::The authors tested a newly-developed computerized laser scanning cytometer (LSC) as a means of performing immunophenotypic analysis of hematologic specimens within their community hospital. Results were compared on a case-by-case basis with parallel flow cytometric and immunohistochemical data. A total of 71 specimens...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/105.6.744
更新日期:1996-06-01 00:00:00
abstract::Adenomatous polyps are common neoplastic lesions of the large intestine. The risk of carcinoma increases with polyp size. Small polyps are typically totally embedded for histologic examination, but no standard method for sampling large, grossly benign polyps has been established. We reviewed grossly noninvasive adenom...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/804U-LHU1-TJ00-UGAU
更新日期:2001-09-01 00:00:00
abstract:OBJECTIVES:In 2001, the University of New Mexico Hospitals implemented a rapid screening tool for the triage of suspected hantavirus cardiopulmonary syndrome based on peripheral blood smear morphology. Five criteria guided clinical decisions: thrombocytopenia, hemoconcentration, granulocytic left shift, absence of toxi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPNFVWG46NUHED
更新日期:2014-08-01 00:00:00
abstract::In 42 patients with locally advanced breast cancer treated with neoadjuvant chemotherapy followed by surgery and radiation therapy, the effects of chemotherapy on tumor architecture, morphometric nuclear and nucleolar characteristics, DNA ploidy, proliferation index measured by mitotic activity index, expression of di...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/107.2.211
更新日期:1997-02-01 00:00:00
abstract::The authors investigated the blast cells obtained from two patients with acute myelofibrosis with the use of recently developed immunochemical and ultrastructural markers. They intended to examine the expression of megakaryoblastic, erythroblastic, myeloblastic, and monoblastic properties in these cells. Respectively,...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.1.114
更新日期:1985-01-01 00:00:00
abstract::Analysis of the data for the 1969 to 1973 CAP Surveys of proficiency in partial thromboplastin time (PTT) determination indicates more than desirable variability in this measurement. Non-activated procedures show greater interlaboratory variability than activated methods; therefore, they may be preferable for routine ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.6.687
更新日期:1990-12-01 00:00:00
abstract:OBJECTIVES:To compare thromboelastography (TEG) tracings obtained from fresh and citrated whole-blood samples in patients on extracorporeal membrane oxygenation (ECMO) or after cardiopulmonary bypass and in healthy volunteers. METHODS:Samples of fresh and citrated whole blood were analyzed for 25 patients and 4 health...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPYIQ9JNNSEN4Q
更新日期:2013-08-01 00:00:00
abstract::An assessment of the infrequently performed protein-bound iodine determinations (PBI) and thyroxine (T4) determinations by column chromatography is presented after analysis of data contributed by participants in the 1976 College of American Pathologists Chemistry Survey Programs. The two technics yield comparable mean...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract:OBJECTIVES:Promotion of high-quality care at a lower cost requires educational initiatives across the continuum of medical education. A needs assessment was performed to inform the design of an educational tool with the goal of teaching laboratory stewardship to medical students. METHODS:The needs assessment consisted...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz102
更新日期:2020-01-01 00:00:00
abstract::We studied 68 sporadic colorectal carcinomas (CRCs) with medullary features (MCRCs) and compared them with 35 poorly differentiated purely "enteric" CRCs (ECRCs) and 15 purely neuroendocrine carcinomas (NECs) of grades II and III, all in patients lacking a family history of CRC. Potential clinicopathologic differences...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.4.537
更新日期:1975-10-01 00:00:00
abstract::Primary massive ovarian edema is uncommon, particularly when it involves both ovaries before puberty. Ovarian edema secondary to a preexisting ovarian lesion is one of the postulated mechanisms. A prepubertal girl presented with bilateral massive ovarian edema with stromal hyperthecosis, which may have predisposed bot...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.5.597
更新日期:1989-05-01 00:00:00
abstract::The results from fetal-maternal hemorrhage (FMH) detection and quantitation external quality assessment surveys conducted in Ontario indicate that the rosette test had a sensitivity and specificity for an FMH of more than 10 mL of 1.0 and 0.75, respectively, compared with 0.96 and 0.92, respectively, for acid elution....
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/3U97-V732-7JBD-PAYN
更新日期:2003-01-01 00:00:00
abstract::Encephalitozoon (Septata) intestinalis affects AIDS patients with CD4 counts <100/microL, causing intestinal and disseminated disease. It must be distinguished from the more common intestinal microsporidian, Enterocytozoon bieneusi, and from other microsporidia of extraintestinal tissues, such as Encephalitozoon helle...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.5.606
更新日期:1996-11-01 00:00:00
abstract::B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/29DA-CY9K-BHNW-4BG6
更新日期:2005-08-01 00:00:00
abstract::Three anti-Factor-VIII antibodies from hemophiliacs were reacted with samples of batches of Maws commerical bovine and porcine Factor VIII concentrates manufactured over a 12-year period. The apparent antibody concentrations varied widely with the different batches of concentrates. The variations are probably due to i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.6.957
更新日期:1976-06-01 00:00:00
abstract::Estrogen receptor (ER), progesterone receptor (PR), and c-ERBB2 (HER2/neu) are therapeutically and prognostically important markers in the management of breast carcinoma. They are not always analyzed in distant metastatic and locally recurrent breast cancers. We compared immunohistochemical expression in a series of p...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPJ57FLLJRXKPV
更新日期:2010-03-01 00:00:00
abstract::The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPQOD9WFPEYY0H
更新日期:2012-10-01 00:00:00
abstract::To investigate the effect of matrix-degrading enzymes on the malignant potential of oral squamous cell carcinoma, the expression of matrix metalloproteinase-2 (MMP-2/72-kD gelatinase/type IV collagenase) in 46 patients who had neck surgery for oral cancer was studied immunohistochemically. In 20 of 26 patients (76.9%)...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/99.1.18
更新日期:1993-01-01 00:00:00
abstract::DNA sequencing was used to confirm Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids in endocervical swab samples. DNA in residues of the samples with positive results by 2 commercial kits was subjected to nested polymerase chain reaction (PCR) amplification. The nested PCR amplicons were used as templates...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/Y2202RVCGRWW1RWB
更新日期:2008-06-01 00:00:00
abstract::The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.1.98
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVES:Blood specimen hemolysis is a major cause of sample recollection in the neonatal intensive care unit. We aimed to reduce the hemolysis rate from 6.3% at baseline to less than 4% within the 9 months' duration of the study. METHODS:Intravenous infusion of lipid emulsion during sample collection, sample collec...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw086
更新日期:2016-07-01 00:00:00
abstract::This study was undertaken to evaluate the precision and sensitivity of three different commercial APTT reagents containing the activators kaolin, micronized silica, or ellagic acid. These reagents varied greatly in their ability to detect mild coagulopathies. The ellagic acid reagent was able to detect the mildest def...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.4.436
更新日期:1984-10-01 00:00:00
abstract::Blood gas measurements performed at the central laboratory and at a satellite laboratory of Brigham and Women's Hospital were studied to determine the differences in quality, turnaround time (TAT), and cost. The quality in both laboratories, as determined by results on proficiency and quality control samples, satisfac...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.1.7
更新日期:1994-07-01 00:00:00
abstract::The authors describe two patients with clinically and histopathologically documented advanced (tumor) stage mycosis fungoides. In each case the large, pleomorphic neoplastic cells lacked the monoclonal antibody-defined cell surface antigens commonly associated with immature and mature T-cells, i.e., T11, Leu-1, T3, T4...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.1.25
更新日期:1986-07-01 00:00:00
abstract::To verify their clinical usefulness in diagnosis and early response to therapy of sideropenic anemia, we compared the behavior of the reticulocyte parameter (RET-Y), a raw measure dependent on size and content of the cell, generated by the Sysmex XE 2100, with the mean reticulocyte volume (MCVr) and mean reticulocyte ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/W652-95DT-UWK7-U1HH
更新日期:2004-04-01 00:00:00
abstract::Proper collection, handling, and storage of the blood alcohol specimen are essential in medicolegal cases involving the question of sobriety. A standard operating procedure is necessary to ensure maximum reliability. Comments are offered on the advantages of using blood specimens in preference to urine or tissue speci...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/74.5.743
更新日期:1980-11-01 00:00:00