Abstract：
:Infusible platelet membranes (IPMs) prepared from fresh or outdated human platelets have been shown to correct prolonged bleeding times in thrombocytopenic rabbits. In previous trials, IPMs did not seem to be immunogenic and lacked dose-limiting toxicity. The present study was undertaken to explore whether the platelet glycoprotein (GP) Ib/IX/V complex might retain functionality in the IPM preparation. IPMs did not spontaneously bind von Willebrand factor (vWF), but saturable binding could be induced by ristocetin, with a dissociation constant (Kd) of 0.31 +/- 0.03 microgram/mL at 1.0 mg/mL of ristocetin. Of 4 anti-GPIb-alpha monoclonal antibodies tested, AN-51 inhibited vWF binding 67.8% +/- 5.8%, whereas AS-2, AS-7, and SZ-2 were ineffective. Maximal vWF binding induced by botrocetin was only 10% to 15% of that observed with ristocetin. Retention of partial functionality of the GPIb/IX/V receptor allowing vWF binding in a modulated manner seems to represent a critical mechanism by which IPMs may provide hemostatic efficacy.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Graham SS,Gonchoroff NJ,Miller JLdoi
10.1309/CCDV-3BEP-XXKP-BKDMsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
144-7issue
1eissn
0002-9173issn
1943-7722journal_volume
115pub_type
杂志文章abstract：:The visible absorption spectra of dilutions of commercially prepared chemistry control sera closely mimic those of jaundiced amniotic fluids in cases of fetal rhesus isoimmunization. The authors have assessed the use of these materials for the quality control of net absorbance measurements at 450 nm on amniotic fluids...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.4.513
更新日期：1985-10-01 00:00:00
abstract：:Immunoperoxidase technics were used to examine the immunoglobulin content of sections of pulmonary tissue from two typical cases of lymphomatoid granulomatosis and two cases of pneumonic processes initially diagnosed as lymphomatoid granulomatosis but representing different processes on review. Both "typical" cases an...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.1.41
更新日期：1980-01-01 00:00:00
abstract：:Labor use ratings assigned to instruments by the Workload Recording Method (WRM) do not change with batch size or walk-away time use. The authors evaluated the effect of both on the labor use of the analyzers Paramax B6100 (Baxter Paramax, Irvine, CA) and Ektachem 700 (Eastman Kodak, Rochester, NY) by timing all worke...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.5.743
更新日期：1991-05-01 00:00:00
abstract：:Twenty-eight resected primary non-small cell lung carcinomas were studied for intratumoral DNA variability by flow cytometry (FCM). Three separate tissue specimens from each resected tumor were divided equally for FCM analysis and histologic evaluation. FCM analysis also was performed on fine-needle aspirates (FNAs) f...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/96.3.311
更新日期：1991-09-01 00:00:00
abstract：OBJECTIVES:Mutations in CALR (calreticulin) have been discovered in 50% to 80% of JAK2 (Janus kinase 2) and MPL (myeloproliferative leukemia protein) wild-type patients with Philadelphia-negative myeloproliferative neoplasm (MPNs). We evaluate the performance of a monoclonal antibody for immunohistochemical detection o...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw135
更新日期：2016-10-01 00:00:00
abstract：:Several technics are currently being used to detect hepatitis type A antigen or its antibody. These include immunoelectronmicroscopy, immune adherence, and complement fixation. This paper describes another promising technic, a microtiter solid-phase immunoradiometric assay, in which hepatitis A antigen and antibody ar...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:A goat anti-serum was prepared against mucinous ovarian cyst fluid and absorbed with normal colon and a variety of normal tissues until the only residual immunoreactivity was directed against colon cancer and ovarian tumor mucin. The set of antigenic determinants defined by this anti-serum has been called COTA, standi...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.1.1
更新日期：1986-07-01 00:00:00
abstract：:A girl, aged 2 years, 11 months, who had pain and swelling of the proximal humerus, was found to have osteoblastic osteogenic sarcoma of the humerus. Among 937 consecutive patients with osteogenic sarcoma examined at the Mayo Clinic, she is the youngest child seen, as well as only the sixth child younger than 6 years...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/63.6.886
更新日期：1975-06-01 00:00:00
abstract：:The authors report a case of primary Ki-1 lymphoma of the brain. The patient was a 4 1/2-year-old black girl who presented with a 4- and 5-day history of headaches, nausea, vomiting, neck stiffness, and difficulty in walking. Computed tomography (CT) scan of the brain showed two discrete densities in the left occipita...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/103.4.496
更新日期：1995-04-01 00:00:00
abstract：:Anaplastic large cell lymphoma (ALCL) is clinically and pathologically heterogeneous, leading to some difficulty in its diagnosis and acceptance as a specific entity. ALCL can be subdivided into different groups according to histologic features (pleomorphic, monomorphic, small cell predominant, Hodgkin's disease-relat...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：:The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVES:The incidence of lymphoproliferative disorders (LPDs) is increasing in sub-Saharan Africa (SSA) due to population growth, aging, and human immunodeficiency virus (HIV). Despite significant burden, resources for diagnosis and treatment of LPDs are limited, with little infrastructure to deliver modern patholog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw118
更新日期：2016-10-01 00:00:00
abstract：:Immunophenotypic analysis is useful in distinguishing reactive from neoplastic lymphoproliferations, particularly when tissue is limited or histologic findings are equivocal. Surface membrane immunoglobulin (SmIg) light chain restriction in B cells is especially helpful in documenting clonality, and the loss of SmIg b...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/5R76B01VCATQLBK6
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVES:Pathogenic free-living amebae (FLAs) cause skin, ocular, and central nervous system (CNS) infections with significant morbidity and mortality. Diagnosis of FLA infections by pathologic examination of tissue sections can be aided using molecular assays. This study investigated the performance characteristics ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz103
更新日期：2019-11-04 00:00:00
abstract：:Adenomatous polyps are common neoplastic lesions of the large intestine. The risk of carcinoma increases with polyp size. Small polyps are typically totally embedded for histologic examination, but no standard method for sampling large, grossly benign polyps has been established. We reviewed grossly noninvasive adenom...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/804U-LHU1-TJ00-UGAU
更新日期：2001-09-01 00:00:00
abstract：:Invasion of the visceral pleura is an important component of lung carcinoma staging, and in some studies is an independent prognostic indicator. Evaluation of invasion by H&E may be indeterminate. Elastic stains can be helpful but are performed rarely. We reviewed all lung carcinoma resections from 1993 for 13 histolo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/112.6.777
更新日期：1999-12-01 00:00:00
abstract：:We describe a method of spectrophotometric detection of BCR/ABL chimeric sequences amplified by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), enabling the use of archival hematologic slides as RNA sources. Multiplex PCR amplified b3a2, b2a2, and e1a2 break points of the BCR/ABL translocation and ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/108.4.383
更新日期：1997-10-01 00:00:00
abstract：:A 73-year-old man with myelodysplastic syndrome developed during the course of his illness auto-immune hemolytic anemia. The association between auto-immune hemolysis and myeloproliferative disease is extremely rare. An even more unique finding is the nature of the antibody which demonstrated single specificity agains...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/77.3.356
更新日期：1982-03-01 00:00:00
abstract：:Examination of urinary sediment has been recommended as a useful diagnostic aid for renal amyloidosis. This procedure was evaluated in the cases of 57 patients, including 11 with proven renal amyloidosis and 13 considered very likely to have the disease. At least three types of urinary fibrillar material were observed...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.1.36
更新日期：1979-01-01 00:00:00
abstract：:Inherited giant platelet disorders are extremely rare. The aim of this article is to review the clinical and laboratory features of this heterogeneous group and to arrive at a working classification. We conducted our literature search using the National Library of Medicine database. A total of 12 clinical entities wer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/FC4H-LM5V-VCW8-DNJU
更新日期：2000-02-01 00:00:00
abstract：:Fine-needle cytology (FNC) of 292 palpable orbital and eyelid tumors was performed with a 25-gauge (0.5-mm), 3-cm needle and compared with the histopathologic findings in 286 cases. Among these 286 cases, a concordant diagnosis of malignancy and type was achieved in 249 cases (87%). False positive diagnoses were made ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/93.1.100
更新日期：1990-01-01 00:00:00
abstract：:Histiocytosis X, multicentric reticulohistiocytosis, juvenile xanthogranuloma, the "fibrous" type of dermatofibroma, dermatofibrosarcoma protuberans, and malignant fibrous histiocytoma are all characterized by dermal and/or subcutaneous infiltrates composed at least partially of cells having morphologic features sugge...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.2.162
更新日期：1981-02-01 00:00:00
abstract：:A 52-year-old woman presented with a solid spindle cell primary tumor of the lymph node. On light microscopic examination, mitoses and cellular atypia were absent. Electron-microscopic studies showed endothelial cell differentiation. Intracytoplasmic localization of Factor VIII-related antigen was demonstrated by the ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.6.731
更新日期：1986-06-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to determine if the Advia 2120 hematology analyzer (Siemens Healthcare Diagnostics, Deerfield, IL) can accurately quantify both WBCs and the proportion of neutrophils from synovial and ascitic body fluids. METHODS:We analyzed 60 samples on the Advia and compared the results wit...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP6JIJV7WYMUAK
更新日期：2013-12-01 00:00:00
abstract：:Urine myoglobin concentrations are measured clinically to assess rhabdomyolysis and the related risk of renal damage. We studied urine myoglobin concentrations in vitro to explore the factors affecting stability. Myoglobin was very unstable in urine specimens, especially below pH 6.5, and its immunoreactivity deterior...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/9AQ6-2FR2-65ER-3E2W
更新日期：2005-03-01 00:00:00
abstract：:The role of viruses in several renal diseases is not documented clearly. The authors attempted to localize envelope glycoproteins of Hantaan virus in biopsy specimens from patients with Korean hemorrhagic fever (KHF) as evidence of direct viral invasion of renal tissues. The authors studied sequential sections of kidn...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.4.398
更新日期：1993-10-01 00:00:00
abstract：:This is a report of the histochemical and ultrastructural analysis of nevus cell aggregates in the placenta from a neonate with a giant pigmented nevus. Although the distinction between aberrant migration of neural crest elements and blood-borne metastasis from the giant pigmented nevus is not readily resolved, the au...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/89.3.395
更新日期：1988-03-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to determine if the diagnosis of nephrogenic adenoma (NA) can be made on cytologic criteria alone and if pair box gene transcription factor 8 (PAX8) is useful in the diagnosis of NA in daily cytology practice. METHODS:Cytologic features of NA previously described in a literature wer...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw006
更新日期：2016-03-01 00:00:00
abstract：:Reported is a case of a rare lymphoepithelial cyst of the tail of the pancreas that developed in a young man with no symptoms. To the authors' knowledge, it is the first case described to be entirely situated within the pancreas, thus confirming its pancreatic origin. Biologic behavior appears to be entirely benign, a...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/98.2.188
更新日期：1992-08-01 00:00:00
abstract：:Recently, strains of Streptococcus pneumoniae with greatly increased resistance to penicillin (minimal inhibitory concentrations or MICs, 1--8 microgram/ml) were recovered in cultures of blood from patients in South Africa and from one in Minnesota who had serious pneumococcal infections. The authors undertook a study...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.2.177
更新日期：1980-02-01 00:00:00
