Abstract：
OBJECTIVES:Mutations in CALR (calreticulin) have been discovered in 50% to 80% of JAK2 (Janus kinase 2) and MPL (myeloproliferative leukemia protein) wild-type patients with Philadelphia-negative myeloproliferative neoplasm (MPNs). We evaluate the performance of a monoclonal antibody for immunohistochemical detection of CALR mutations. METHODS:A computerized archival search was performed for cases of non-chronic myeloid leukemia (CML) MPNs with available CALR and JAK2 V617F mutational analysis data. Bone marrow biopsy specimens were stained with monoclonal antibody CAL2, and the percentage of stained megakaryocytes was calculated. In select cases, double immunofluorescence staining was done with CAL2 and each of the following: CD61, myeloperoxidase, CD34, and glycophorin A. RESULTS:We studied 38 bone marrow biopsy specimens of non-CML MPNs (primary myelofibrosis, n = 21; essential thrombocythemia, n = 15; and n = 2 post-polycythemia vera myelofibrosis) from 31 patients. All eight bone marrow biopsy specimens from patients with mutant CALR showed strong cytoplasmic staining of the megakaryocytes (83.5%; range, 50%-98%; median, 87%) with the CAL2 antibody. Double immunofluorescence staining of the small mononuclear cells seen in CALR mutant cases revealed them to be myeloid blasts. CONCLUSIONS:Immunohistochemistry in routinely processed bone marrow biopsy specimens for mutated CALR is feasible and accurately identifies mutated cases, including rare cases with additional driver mutations.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Nomani L,Bodo J,Zhao X,Durkin L,Loghavi S,Hsi EDdoi
10.1093/ajcp/aqw135subject
Has Abstractpub_date
2016-10-01 00:00:00pages
431-8issue
4eissn
0002-9173issn
1943-7722pii
aqw135journal_volume
146pub_type
杂志文章abstract：Objectives:Hemolyzed blood samples commonly occur in hospital emergency departments (EDs). Our objective was to determine whether replacing standard large-volume/high-vacuum sample tubes with low-volume/low-vacuum tubes would significantly affect ED hemolysis. Methods:This was a prospective intervention of the use of ...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章
doi：10.1093/ajcp/aqx082
更新日期：2017-10-01 00:00:00
abstract：:Cytologic features of the cell-stroma interface are useful in distinguishing between monomorphic adenomas of the basal cell type and adenoid cystic carcinoma. In basal cell adenomas, the collagenous stroma interdigitates with adjacent cells, whereas in adenoid cystic carcinoma, the two are separated by a sharp smooth ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/106.1.35
更新日期：1996-07-01 00:00:00
abstract：:A very simple method of computer-assisted reporting of surgical pathology (including gross description, microscopic description, and diagnosis) has been developed. This method is based on a modification of the existing microbiology program and files in the comprehensive MedLab Company Pathlab computer system, with the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/78.3.337
更新日期：1982-09-01 00:00:00
abstract：:The aims of this study were to compare the quality of immunohistochemical assays of estrogen receptor (ER) and progesterone receptor (PR) and to compare the intermethod variability of assays from different manufacturers. immunohistochemical staining was entrusted to the following laboratories in Japan: Kyowa Medex, de...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/4D1A04NCDK96WFY7
更新日期：2007-03-01 00:00:00
abstract：:We reviewed 500 consecutive soft tissue lesions referred for expert consultation to determine types of lesions and/or situations in which major discrepancies occur. Of 266 cases (53.2%) accompanied by a diagnosis, essential agreement with the second opinion was noted in 68%, minor discrepancy in 7%, and major discrepa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/425H-NW4W-XC9A-005H
更新日期：2001-10-01 00:00:00
abstract：:We aimed to study the expression and localization of the molecular components of enterocyte junctions in celiac disease together with the level of tyrosine phosphorylation, a phenomenon known to affect their cellular distribution and function, and to explore the influence of proinflammatory cytokines. Duodenal biopsy ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/DTYR-A91G-8R0K-TM8M
更新日期：2006-04-01 00:00:00
abstract：:To investigate the association between the positive direct antiglobulin test (DAT) and hypergammaglobulinemia, the authors prospectively studied 154 patients, classified into three groups: Group 1, 52 patients with a positive DAT result in pretransfusion samples; Group 2, 52 patients with a negative DAT result; and Gr...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.2.197
更新日期：1988-08-01 00:00:00
abstract：:We compared an automated quantitative heart-type fatty acid-binding protein (H-FABP) assay with other cardiac-marker assays to examine its usefulness as an early diagnostic marker of acute myocardial infarction (AMI). Serum samples for cardiac troponin T (cTnT), creatine kinase-MB isozyme (CK-MB), myoglobin, and H-FAB...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCP0F6AXRCJMQQG
更新日期：2010-07-01 00:00:00
abstract：:Carumonam, a new monobactam, was found to have an anti-microbial spectrum similar to aztreonam. Its spectrum includes Enterobacteriaceae, Haemophilus influenzae, pathogenic Neisseria species, Pseudomonas aeruginosa, and some streptococci. Staphylococcus species, enterococci, and many other nonenteric gram-negative bac...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.5.608
更新日期：1986-11-01 00:00:00
abstract：:The standard method for detecting antinuclear antibodies (ANAs) is by immunofluorescence assay (IFA), a method that is labor intensive and subjective. In an attempt to overcome these limitations, several commercial enzyme immunoassays (EIAs) have been developed. We report the results of our evaluation of the ANA Micro...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/111.4.503
更新日期：1999-04-01 00:00:00
abstract：:Campylobacter fetus subspecies jejuni has recently been recognized as a significant cause of enteritis in humans. The organism has been previously postulated to cause illness by direct invasion of the mucosa of the gastrointestinal tract, but this has not been documented. Reported is a case of Campylobacter enteritis ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/73.5.706
更新日期：1980-05-01 00:00:00
abstract：OBJECTIVES:Meningiomas usually can be readily diagnosed on H&E alone, although occasionally immunohistochemistry (IHC) confirmation is desirable. Studies exploring the diagnostic utility of either podoplanin (D2-40) or E-cadherin IHC in meningiomas have conflicted, and no studies exist in which the two IHCs have been u...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPSULJ6DW5REHR
更新日期：2015-12-01 00:00:00
abstract：:Primary malignant germ cell tumors of the mediastinum are extremely rare among females. This report describes such a tumor arising in the mediastinum of a 26-year-old woman and correlates immunohistochemical studies of the neoplasm with serum tumor markers. Microscopic examination of the tumor revealed an embryonal ca...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/75.4.573
更新日期：1981-04-01 00:00:00
abstract：:We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/4QUN-J4F2-7QK7-RR0G
更新日期：2004-11-01 00:00:00
abstract：:A tumor of the larynx composed of a small infiltrating and well-differentiated squamous cell carcinoma and a large polypoid sarcomatous mass showing the histologic features of the malignant giant cell tumor of soft parts (MGCT) is reported. Other types of sarcomatous components have been described with squamous cell c...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/82.3.340
更新日期：1984-09-01 00:00:00
abstract：OBJECTIVES:Tissue confirmation of the intrauterine location of a pregnancy can be difficult grossly, necessitating a frozen section diagnosis. First-trimester tissue is often scant and can be exhausted by frozen section. We examined 39 endometrial curettings performed for rule-out ectopic pregnancy by touch prep to exa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw048
更新日期：2016-06-01 00:00:00
abstract：:Acute paraplegia developed in a 53-year-old man with pulmonary aspergilloma because of contiguous extension of Aspergillus infection to the epidural and subdural spaces and spinal cord. Histopathologic findings of the spinal cord showed Aspergillus hyphae penetrating the myelin sheath and myelomalacia, predominantly i...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/84.6.763
更新日期：1985-12-01 00:00:00
abstract：:A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/64.4.537
更新日期：1975-10-01 00:00:00
abstract：:Blood gas measurements performed at the central laboratory and at a satellite laboratory of Brigham and Women's Hospital were studied to determine the differences in quality, turnaround time (TAT), and cost. The quality in both laboratories, as determined by results on proficiency and quality control samples, satisfac...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.1.7
更新日期：1994-07-01 00:00:00
abstract：OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw105
更新日期：2016-08-01 00:00:00
abstract：:A number of genetic abnormalities have been detected in multiple myeloma (MM) using cytogenetic techniques. The prominent abnormalities are deletions of 13q and translocations affecting the IgH locus on 14q32. The recurrence of chromosomal abnormalities in MM suggests a specific role for them concerning its pathogenes...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/5KWK-P6UK-GNXX-HMYH
更新日期：2004-12-01 00:00:00
abstract：:A girl, aged 2 years, 11 months, who had pain and swelling of the proximal humerus, was found to have osteoblastic osteogenic sarcoma of the humerus. Among 937 consecutive patients with osteogenic sarcoma examined at the Mayo Clinic, she is the youngest child seen, as well as only the sixth child younger than 6 years...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/63.6.886
更新日期：1975-06-01 00:00:00
abstract：:Fatty acid synthase is an enzyme that catalyzes the synthesis of long-chain fatty acids. The enzyme expression is minimal in adult tissues and very high in many cancers. Ulcerative colitis is a chronic inflammatory bowel disease that, when long-standing, is associated with an increased risk of colon cancer. The aim of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/pubvqndnvqkjvc8m
更新日期：2006-07-01 00:00:00
abstract：:Increasingly, all automated blood counts are not accompanied by a microscopic white blood cell differential. A popular strategy is to obtain a manual differential if any part of the automated blood count and differential is outside specified limits (the "diff-if" strategy). The authors compared two sets of criteria to...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/90.2.163
更新日期：1988-08-01 00:00:00
abstract：:The availability of automated anti-Xa heparin assays provides the opportunity to manage patient unfractionated heparin levels directly, rather than by the activated partial thromboplastin time. Because critically ill patients can acquire an antithrombin deficiency, we compared the performance of 3 anti-Xa heparin assa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/8E3U7RXEPXNP27R7
更新日期：2006-09-01 00:00:00
abstract：:To investigate the specificity of terminal deoxynucleotidyl transferase (TdT) as a marker for lymphoblastic lymphoma in the non-Hodgkin's lymphomas, the authors assayed malignant cells from 142 patients with non-Hodgkin's malignant lymphoma, as well as cells from normal lymphoid tissue controls. Neoplastic cells from ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.5.655
更新日期：1983-11-01 00:00:00
abstract：OBJECTIVES:Fundic gland polyps (FGPs) occur in two clinical settings, sporadic and syndromic. Epithelial dysplasia is rare in sporadic FGPs, and progression data from large series are lacking. The aim of this study was to evaluate the clinical, morphologic, and immunohistochemical features in a large series of sporadic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPGK8QTYPUQJYL
更新日期：2015-10-01 00:00:00
abstract：:This study assessed the value of argyrophilic nucleolar organizer region (AgNOR) staining as a potential technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Using a silver staining technique and immunohistochemistry, the authors correlated the numbe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.4.401
更新日期：1989-10-01 00:00:00
abstract：:While plasmacytosis is a common occurrence in early post-bone marrow transplantation biopsy specimens, the significance of plasma cells in such specimens from patients with multiple myeloma (MM) is unknown. We attempted to retrospectively determine, by morphologic assessment of plasma cell percentage, immunohistologic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.6.643
更新日期：1997-06-01 00:00:00
abstract：:Uterine tumors with histologic resemblances to ovarian sex-cord tumors were encountered in 14 women in the fourth to sixth decades of life, most of whom had abnormal vaginal bleeding and enlarged uteri suggesting leiomyomas. The tumors in six cases (Group I) were identical to one or another form of well-recognized end...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/66.3.512
更新日期：1976-09-01 00:00:00
