Theory and applications of the polymerase chain reaction.

Abstract:

:The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such that a million-fold amplification is possible. This powerful method has numerous applications in diagnostic pathology, especially in the fields of microbiology, forensic science, and hematology. The PCR may be used to directly detect viral DNA, which may facilitate the diagnosis of acquired immune deficiency syndrome (AIDS) or other viral diseases. PCR amplification of DNA allows detection of specific sequences from extremely small samples, such as with forensic material. In hematology, PCR may help in the diagnosis of hemoglobinopathies or of neoplastic disorders by documenting chromosomal translocations. The new PCR opens exciting new avenues for diagnostic pathology using this new technology.

journal_name

Am J Clin Pathol

authors

Remick DG,Kunkel SL,Holbrook EA,Hanson CA

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

S49-54

issue

4 Suppl 1

eissn

0002-9173

issn

1943-7722

journal_volume

93

pub_type

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