Spurious elevation of the electronically determined mean corpuscular volume and hematocrit caused by hyperglycemia.

Abstract:

:Following normalization of blood glucose levels, a marked change in the MCV and in the calculated hematocrit was observed in five patients with transient severe hyperglycemia. The addition of varied concentrations of glucose to blood samples from healthy volunteers and from patients with microcytic, macrocytic, and normocytic anemia produced a similar and proportionate increase in the MCV. Increases in urea concentration to clinically relevant levels had no effect. The phenomenon appears to result from the osmotic swelling and subsequent shrinking of hyperglycemia erythrocytes when they are mixed with the diluent used for measurement of hematologic parameters. The magnitude of this spurious macrocytosis is less pronounced with the Coulter S than with the Coulter S-Plus. This difference can be explained by the differences in incubation prior to measurement of these instruments. A method for eliminating this spurious finding in blood samples with high levels of glucose is recommended.

journal_name

Am J Clin Pathol

authors

Holt JT,DeWandler MJ,Arvan DA

doi

10.1093/ajcp/77.5.561

subject

Has Abstract

pub_date

1982-05-01 00:00:00

pages

561-7

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

77

pub_type

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