Abstract：
:The development of a consistent strategy for the analysis of oncogene expression at the cellular level is essential for understanding the roles of these genes in the development and progression of human neoplasia. Detection of the neu oncogene products in breast carcinoma was selected as a model for analysis of oncogene expression. Fifty-two primary human breast carcinomas were evaluated by quantitation of neu DNA amplification and mRNA expression and by localization of neu mRNA and protein (p 185) at the cellular level by in situ hybridization (ISH) and immunohistochemistry (IHC). The specificity and sensitivity of the molecular and immunologic probes for neu were established with the use of genetically engineered cell lines that overexpressed either neu or epidermal growth factor receptor (EGFR). Twenty-nine percent of breast carcinomas demonstrated neu DNA amplification and mRNA overexpression, and there was close correlation between the level of neu mRNA expression and detection of neu gene products by ISH and IHC. Thirty-two percent of carcinomas demonstrated neu mRNA overexpression by ISH. The immunohistochemical method using TA1 monoclonal antibody for p185 was exquisitely sensitive in acetone-fixed frozen sections and provided an excellent approach for judging overexpression as confirmed by the various molecular analyses. All areas of nonmalignant breast epithelium stained weakly, and a wide range of staining intensity was observed in malignant breast epithelium, with 31% of carcinomas judged to be p185 overexpressors. Heterogeneous expression of p185 was seen in some carcinomas. This study provides a strategic approach for the evaluation of oncogene expression in human tumors.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Naber SP,Tsutsumi Y,Yin S,Zolnay SA,Mobtaker H,Marks PJ,McKenzie SJ,DeLellis RA,Wolfe HJdoi
10.1093/ajcp/94.2.125subject
Has Abstractpub_date
1990-08-01 00:00:00pages
125-36issue
2eissn
0002-9173issn
1943-7722journal_volume
94pub_type
杂志文章abstract：OBJECTIVES:Method imprecision, error rates, and explanatory causes that were identified in the Institute for Quality Management in Healthcare point-of-care (POC) glucose proficiency testing (PT) program were assessed in comparison with results obtained from laboratory glucose PT surveys. METHODS:POC and laboratory glu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPP5YS2MVSKBYY
更新日期：2014-12-01 00:00:00
abstract：:Kikuchi's lymphadenitis is a self-limiting condition typically affecting young patients. Surgical biopsy is unnecessary if a firm diagnosis can be rendered by fine-needle aspiration cytology (FNAC). The authors report the FNAC findings for 27 cases, including 24 female and 3 male patients aged 12 to 43 years. Histolog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.4.454
更新日期：1994-10-01 00:00:00
abstract：:p53 protein expression was evaluated in a series of 204 primary colorectal adenocarcinomas by immunohistochemistry using frozen tissue sections and monoclonal antibody DO-7. Nuclear staining of more than 5% of neoplastic cells was observed in 124 (60.8%) adenocarcinomas, which were classified as p53 positive. p53 immu...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/105.5.604
更新日期：1996-05-01 00:00:00
abstract：:CD45/side scatter (SS) gating is widely used for isolating blasts by flow cytometry (FC). However, other cells contaminate the "blast gate" (BG); CD45/SS gating is thus imprecise, particularly when there are few blasts. We studied the BG contents in 21 myelodysplastic syndromes (MDSs), 14 myeloproliferative neoplasms ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPN4G1IZPABRLH
更新日期：2012-05-01 00:00:00
abstract：:Extramammary Paget disease (EPD) is an uncommon cutaneous malignant neoplasm that arises in areas rich in apocrine glands (perineum, vulva, and axilla). Apocrine gland origin or apocrine differentiation of cells of EPD has been suggested. Estrongen, progesterone, and androgen hormone receptors have been reported to ex...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/P756-XXCB-TV71-U4XV
更新日期：2000-04-01 00:00:00
abstract：:Cytokeratin (CK) expression was studied in 22 sinonasal inverted papillomas. Columnar (respiratory) epithelium in inverted papillomas abundantly expressed CK7, CK8, CK18, and CK19. Immunoreactivity for CK5/14 and CK17 was found in basal and parabasal/suprabasal cells. Transitional (cuboidal) and squamous epithelium in...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TH5J-T1Q6-9QJR-8CDK
更新日期：2001-05-01 00:00:00
abstract：:The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/94.6.687
更新日期：1990-12-01 00:00:00
abstract：:Pseudomonas alcaligenes is a common soil and water inhabitant that has rarely been proven a human pathogen. We describe a fatal case of Pseudomonas alcaligenes endocarditis. The need for accurate identification of unusual organisms isolated in a clinical setting are discussed. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/79.2.245
更新日期：1983-02-01 00:00:00
abstract：:Diarrheal diseases caused by bacterial pathogens are important causes of morbidity in the United States, and considerable laboratory resources are spent to detect these enteric pathogens. This article reviews recent developments in the detection and identification of Campylobacter spp, enterohemorrhagic Escherichia co...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1994-04-01 00:00:00
abstract：:Dialogue regarding the autopsy has been sustained largely by medical students and professionals, and by families of deceased patients. Exclusion of the general public may represent a significant reason for the inexorable decline of autopsy rates and politicoeconomic support. Sensitivity to public concerns regarding th...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:Therapeutic control achieved by an anticoagulant clinic may be assessed by calculation of the proportion of time spent by the patients within the prothrombin time target range aimed for by the clinic. Three calculation methods for such assessment have been described by other investigators. The three methods were compa...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1093/ajcp/90.6.685
更新日期：1988-12-01 00:00:00
abstract：OBJECTIVES:Timely reporting of immunosuppressant (ISP) drug level results is needed for transplant patient management. This study characterized the local ISP testing process, identified bottlenecks and implemented process improvements to meet turnaround time requirements. METHODS:Laboratory information time stamps, di...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw087
更新日期：2016-08-01 00:00:00
abstract：:A survey of adult patients admitted to a general hospital showed that 1.5% had hepatitis B surface antigen in the blood and 22% had hepatitis antibody. The majority of patients with hepatitis B did not have clinical hepatitis and would not have been recognized without screening all hospital admissions for hepatitis B ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/67.3.257
更新日期：1977-03-01 00:00:00
abstract：:Several adulterants are used to mask tests for abused drugs in urine. Adulterants such as "Klear" and "Whizzies" contain potassium nitrite, and "Urine Luck" contains pyridinium chlorochromate (PCC). The presence of these adulterants cannot be detected by routine specimen integrity checks (pH, specific gravity, and tem...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/9Q2G-6CTH-XT16-HCCC
更新日期：2002-02-01 00:00:00
abstract：:The performance of the Coulter STKS (Coulter, Hialeah, FL) was evaluated in a busy computerized teaching hospital laboratory. The STKS was compared with a Coulter S Plus IV and manually performed 400 white blood-cell differentials. The measured blood-count parameters (i.e., white blood cells [WBCs], red blood cells [R...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/95.2.207
更新日期：1991-02-01 00:00:00
abstract：:Medicare policies have gradually restricted the scope of pathologist services payable under the rules applicable to physician services. Recent changes applying relative value scales has provided an opportunity for national standardization of pathology codes, and has permitted the introduction of clinical pathology int...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：OBJECTIVES:To explore the survival and definition of stage IA mixed endometrial carcinoma. METHODS:From June 1, 2010, to June 1, 2017, cases with stage IA endometrial cancer were included in this study. The survival outcomes were compared among patients with endometrioid (group A), nonendometrioid (group B), and mixed...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz083
更新日期：2019-10-07 00:00:00
abstract：:The authors report the clinicopathologic features of 14 cases of amyloid goiter (AG). Eleven patients were males and three females with ages ranging from 23 to 75 years (median, 54 years). Eight patients had secondary amyloidosis and six had primary amyloidosis. Nine cases were identified at autopsy. In symptomatic pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/104.3.306
更新日期：1995-09-01 00:00:00
abstract：:Two unusual cases of acute lymphoblastic leukemia-hand mirror variant (ALL-HMV) are presented. One patient demonstrated a mixed immunophenotype with HLA-DR, My7, transferrin receptor surface markers, and terminal deoxynucleotidyl transferase positivity. To the authors' knowledge, this is the first ALL-HMV reported wit...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.4.410
更新日期：1989-04-01 00:00:00
abstract：:Proteomic studies of cells via surface-enhanced laser desorption/ionization spectrometry (SELDI) analysis have enabled rapid, reproducible protein profiling directly from crude samples. We applied this technique to archival cytology material to determine whether distinct, reproducible protein fingerprints could be ide...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/EJKL-7328-KFPR-56WA
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVES:To evaluate the predictive value of growth patterns in patients undergoing sorafenib treatment for metastatic clear cell renal cell carcinomas (CCRCCs). METHODS:Forty-eight patients were analyzed, each of whom underwent nephrectomy and received sorafenib treatment for metastatic CCRCC. Progression-free surv...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPM8RJ2ECAWOGQ
更新日期：2015-04-01 00:00:00
abstract：:Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/97.1.108
更新日期：1992-01-01 00:00:00
abstract：:To investigate the effect of matrix-degrading enzymes on the malignant potential of oral squamous cell carcinoma, the expression of matrix metalloproteinase-2 (MMP-2/72-kD gelatinase/type IV collagenase) in 46 patients who had neck surgery for oral cancer was studied immunohistochemically. In 20 of 26 patients (76.9%)...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/99.1.18
更新日期：1993-01-01 00:00:00
abstract：:The authors analyzed the frequency of immunophenotypic abnormalities in 1,474 cases of routinely fixed, paraffin-embedded B-lineage non-Hodgkin's lymphomas. B-lineage was determined by immunoreactivity for CD20 (L26, 92%); CD45RA (4KB5, an additional 3%) or immunoglobulin (Ig) light chain restriction (remaining 5%). C...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/102.6.825
更新日期：1994-12-01 00:00:00
abstract：:Cytogenetic studies of bone marrow were performed in 230 consecutive cases of patients with chronic granulocytic leukemia (CGL) admitted to the National Cancer Institute since 1961. Twenty patients lacked the Philadelphia (Ph1) chromosome. All were previously untreated. When compared with Ph1-positive patients, CGL pa...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/65.4.467
更新日期：1976-04-01 00:00:00
abstract：:Fatty acid synthase is an enzyme that catalyzes the synthesis of long-chain fatty acids. The enzyme expression is minimal in adult tissues and very high in many cancers. Ulcerative colitis is a chronic inflammatory bowel disease that, when long-standing, is associated with an increased risk of colon cancer. The aim of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/pubvqndnvqkjvc8m
更新日期：2006-07-01 00:00:00
abstract：:Cardiac-related death has been reported following strenuous exercise, and biochemical markers predicting adverse outcomes would be useful. Despite the fact the myeloperoxidase (MPO) release may precede myocardial injury and identify at-risk patients earlier than traditional markers, information on the effects of marat...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/1D62H6KRFTVQRJ0A
更新日期：2006-12-01 00:00:00
abstract：:A 76-year-old white man previous diagnosed as having Waldenstrom's macroglobulinemia continued with persistent fevers and sweats for two and a half years. Recently, repeated automated differentials during 11 days of hospitalization failed to note any intracellular inclusions in the RBCs. Blood sent to the Microbiology...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/83.4.520
更新日期：1985-04-01 00:00:00
abstract：:Factitious laboratory results often lead to unnecessary testing or treatment. This brief review of factitious biochemical results due to preexisting hematologic conditions focuses on the mechanisms underlying the factitious results and suggests ways to prevent them. An observant pathologist identifies these errors, in...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/AJCPY9RP5QYTYFWC
更新日期：2009-02-01 00:00:00
abstract：:The prehospital environment has not been studied for point-of-care testing. Therefore, the authors' helicopter program evaluated the performance of the i-STAT Portable Clinical Analyzer, a rapid point-of-care, hand-held instrument. The primary aim of the study was to determine if the i-STAT Portable Clinical Analyzer ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
