Abstract:
:The performance of the Coulter STKS (Coulter, Hialeah, FL) was evaluated in a busy computerized teaching hospital laboratory. The STKS was compared with a Coulter S Plus IV and manually performed 400 white blood-cell differentials. The measured blood-count parameters (i.e., white blood cells [WBCs], red blood cells [RBCs], hemoglobulin [Hb], mean corpuscular volume [MCV], and platelets [PLTs]), compared very well between the two aperture impedance-based systems; precision, linearity, and lack of carryover were excellent. The STKS WBC differential (DIFF), derived from a combination of aperture impedance, aperture conductance, and laser light scatter, also was precise; linear and carryover were insignificant. The DIFFs (n = 424) compared well to the manual WBC differentials, with r values of 0.97, 0.97, 0.73, and 0.86 for neutrophils, lymphocytes, monocytes, and eosinophils, respectively. The DIFF and Suspect Flagging system produced 6.2% false negatives and 2.6% false positives when compared with the manual technique. These were further investigated and discussed. STKS DIFFs were stable for 18 to 24 hours in normal samples anticoagulated with K2EDTA and stored at 20 degrees C prior to analysis. Storage in the same anticoagulant at 4 degrees C and immediate aspiration preserved the DIFF analysis for considerably longer than 24 hours. These performance characteristics make the STKS a significant advancement in automated hematology.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Warner BA,Reardon DMdoi
10.1093/ajcp/95.2.207subject
Has Abstractpub_date
1991-02-01 00:00:00pages
207-17issue
2eissn
0002-9173issn
1943-7722journal_volume
95pub_type
杂志文章abstract::The standard method for detecting antinuclear antibodies (ANAs) is by immunofluorescence assay (IFA), a method that is labor intensive and subjective. In an attempt to overcome these limitations, several commercial enzyme immunoassays (EIAs) have been developed. We report the results of our evaluation of the ANA Micro...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/111.4.503
更新日期:1999-04-01 00:00:00
abstract::Anaplastic large cell lymphoma (ALCL) is clinically and pathologically heterogeneous, leading to some difficulty in its diagnosis and acceptance as a specific entity. ALCL can be subdivided into different groups according to histologic features (pleomorphic, monomorphic, small cell predominant, Hodgkin's disease-relat...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVES:We evaluated efficacy of two dual immunohistochemistry (IHC) staining assays in assessing hairy cell leukemia (HCL) involvement in core biopsies and compared the results with concurrently collected flow cytometric data. METHODS:Overall, 148 patients with HCL (123 male, 25 female; mean age: 59.8 years; range...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz171
更新日期:2020-02-08 00:00:00
abstract::Thirty drugs used primarily in critical care and hospital settings were tested in vitro to observe interference on glucose measurements with 6 hand-held glucose meters and a portable glucose analyzer. Paired differences of glucose measurements between drug-spiked samples and unspiked control samples were calculated to...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/QAW1-X5XW-BVRQ-5LKQ
更新日期:2000-01-01 00:00:00
abstract::We compared immunohistochemical expression of hepatocyte growth factor (HGF), c-met, alpha-inhibin, estrogen receptor (ER), and progesterone receptor (PR) in 10 pancreatic mucinous cystic neoplasms (PMCNs), 8 hepatobiliary cystadenomas (HBCs), and 6 simple liver cysts (SLCs).All HBCs and PMCNs were in women (mean ages...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/U2BBP4EMBAHCM6E6
更新日期:2008-02-01 00:00:00
abstract::A case is presented of an anterior mediastinal mass arising in a 64-year-old woman that showed on histologic, immunopathologic, and ultra-structural examination features of a poorly differentiated (insular) thyroid carcinoma. The tumor in this patient most likely arose from ectopically displaced thyroid tissue on the ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.4.408
更新日期:1995-10-01 00:00:00
abstract::Overexpression and amplification of the HER-2 oncogene in patients with breast cancer has correlated with early onset of metastasis, resistance to hormonal therapy and some forms of chemotherapy, and shortened survival. Therefore, evaluation of this putative prognostic or predictive factor seems critical. Because diff...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/0F58-0GRX-FK4R-A6VA
更新日期:2000-05-01 00:00:00
abstract::Lesions of the breast containing extravasated mucin span a continuum from benign mucoceles to invasive mucinous (colloid) carcinoma. It is well known that distinguishing benign from malignant mucinous lesions is difficult infine-needle aspiration material. Whether these lesions also are difficult to distinguish in cor...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/TP6Y-Q0QR-XXVQ-9MJ5
更新日期:2002-07-01 00:00:00
abstract::Loss of intercellular adhesion facilitates tumor invasion. To clarify the relation between altered expression of cell adhesion molecules and progression of T1 superficial bladder tumors, 101 cases (71 T1 tumors, 30 T2/T3 tumors) were examined immunohistochemically for E-cadherin and alpha-, beta-, and gamma-catenins. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::A 55-year-old man presented with nerve compression and examination of tissue removed by laminectomy, and bone marrow aspiration was diagnostic of multiple myeloma. Protein studies showed a total serum protein of 5.7 g/dL, with a M-component in the fast beta region. The abnormal protein reacted only with anti-lambda an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Borrelia burgdorferi is difficult to detect in routine biopsy material from patients with skin lesions of borreliosis. In this study, a new immunohistochemical method, focus floating microscopy (FFM), was developed to detect B burgdorferi in tissue sections and was compared with polymerase chain reaction (PCR). By usi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/3369XXFPEQUNEP5C
更新日期:2007-02-01 00:00:00
abstract::Five patterns of bone marrow infiltration by non-Hodgkin lymphoma or Hodgkin lymphoma are currently recognized, but a true follicular pattern of bone marrow involvement by follicular lymphoma has not been described. In 260 bone marrow trephine biopsy specimens involved by follicular lymphoma, we identified 12 cases wi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/EG2M-YHB9-WEFW-7H1R
更新日期:2002-11-01 00:00:00
abstract::The p63 gene, a member of the p53 family, is an epithelial marker expressed in embryonic ectoderm, breast myoepithelium, prostate, oral epithelium, epidermis, and urothelium. The DeltaN-p63 isoforms of p63, which are believed to behave as oncogenes, are expressed in squamous cell carcinoma, basal cell carcinoma, and t...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/2GAYKPDDM85P2VEW
更新日期:2007-03-01 00:00:00
abstract::A single oral dose of tetracycline deposits in bone and is readily identified with fluorescence microscopy. Two such time-spaced labels can be used to determine bone dynamic features. This is as accurate as conventional three-day labeling periods. The simplicity, improved compliance, and substantial reduction in time ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/91.6.682
更新日期:1989-06-01 00:00:00
abstract::The cytologic features of fine-needle aspiration specimens from 16 breast carcinomas that closely simulated benign lesions were analyzed and compared to smears from fibroadenomas and fibrocystic change. No combination of features was found that accurately separated all benign and malignant cases. Many nuclei with disc...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/98.2.155
更新日期:1992-08-01 00:00:00
abstract::A proportion of patients under surveillance for recurrent bladder carcinoma with no immediate evidence of bladder tumor recurrence have positive multitarget fluorescence in situ hybridization (FISH; UroVysion, Vysis, Downers Grove, IL) results. The course of these "anticipatory positive" cases and the time to bladder ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/ADJL7E810U1H42BJ
更新日期:2007-02-01 00:00:00
abstract::Thirty-five consecutive renal biopsy specimens were evaluated by light histology using paraffin and glycol methacrylate embedding. The clarity of the renal structures was found to be much better, and the renal lesions better defined, using glycol methyacrylate embedding. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.5.655
更新日期:1975-11-01 00:00:00
abstract:OBJECTIVES:To describe four cases of an uncommon type of acalculous cholecystitis/cholangitis characterized by increased intraepithelial lymphocytes within the biliary epithelium. METHODS:Cases were prospectively compiled during regular surgical pathology sign-out. Clinical information was obtained from the electronic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP9Q4QWZYYGDNA
更新日期:2015-01-01 00:00:00
abstract::The clinical, light microscopic, and immunohistochemical features of 14 sinonasal malignant melanomas were studied to show their diverse morphologic appearance and distinction from therapeutically more amenable neoplasms that occur in this region. The tumors arose in 6 men and 8 women (median age, 70 years). Eleven pa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.6.689
更新日期:1991-12-01 00:00:00
abstract:Objectives:Induction chemotherapy (IC) is occasionally used in head and neck cancer, leading to less extensive surgery and reduced need for irradiation. Factors predicting the response to IC have not been determined. In this study, we investigated the clinical and histopathologic factors that predict the response to IC...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw145
更新日期:2016-11-01 00:00:00
abstract::Tissue repositories maintained by pathology departments represent an abundant resource of clinically annotated human specimens. The storage expenses associated with pathology archives are known to administrators of most pathology departments. However, such basic repository characteristics as the quality of stored mate...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP3CVA2NAVUUVU
更新日期:2011-05-01 00:00:00
abstract::The aims of this study were to compare the quality of immunohistochemical assays of estrogen receptor (ER) and progesterone receptor (PR) and to compare the intermethod variability of assays from different manufacturers. immunohistochemical staining was entrusted to the following laboratories in Japan: Kyowa Medex, de...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4D1A04NCDK96WFY7
更新日期:2007-03-01 00:00:00
abstract::A unilateral multicystic mass in the connective tissue adjacent to the vas deferens in a 46-year-old man is reported. Anatomically, it was distinct from the epididymis and vas and contained no sperm. The epithelial lining was simple, ciliated, and cuboidal to columnar. The cysts were surrounded by connective tissue co...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/101.4.543
更新日期:1994-04-01 00:00:00
abstract::Lean methodologies have been applied in many industries to reduce waste. We applied Lean techniques to redesign laboratory workstations with the aim of reducing the number of times employees must leave their workstations to complete their tasks. At baseline in 68 workflows (aggregates or sequence of process steps) stu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPE0PI2ENWYWMU
更新日期:2012-08-01 00:00:00
abstract::The experience at one institution concerning the diagnosis of pulmonary tuberculosis by aspiration biopsy is reviewed. Twelve cases in which acid-fast bacilli were identified in pulmonary aspiration material are reported. The cytologic findings were confirmed by microbiologic culture in nine cases. The advantages of u...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.6.719
更新日期:1985-06-01 00:00:00
abstract::Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatog...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/104.1.89
更新日期:1995-07-01 00:00:00
abstract:Objectives:To determine the impact of systemwide charge display on laboratory utilization. Methods:This was a randomized controlled trial with a baseline period and an intervention period. Tests were randomized to a control arm or an active arm. The maximum allowable Medicare reimbursement rate was displayed for tests...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,随机对照试验
doi:10.1093/ajcp/aqx110
更新日期:2017-11-20 00:00:00
abstract::Human papillomavirus (HPV) testing is more sensitive for the detection of cervical precancer and cancer than cervical cytology. The increased sensitivity of HPV testing and cytology combined ("cotesting") compared to cytology alone permitted professional societies to recommend 3-year screening intervals among the cote...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCPRI8XPQUEAA3K
更新日期:2010-08-01 00:00:00
abstract::An unusual case of carcinoma of the prostate with metastases is described. the prostate and the metastases showed adenocarcinoma with carcinoid-like areas. A tumor with the same histologic features was found at the tip of the appendix and proved to be metastatic. The possibility of primary carcinoid of the prostate wa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.1.94
更新日期:1981-07-01 00:00:00
abstract::Using digital cell image analysis performed on Feulgen-stained nuclei, the nuclear characteristics of prostatic neoplasia, ranging from benign (benign prostatic hyperplasia [BPH]), through dysplastic (prostatic intraepithelial neoplasia [PIN] 1-3), to carcinoma were studied. Four histopathologic groups were studied: g...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.5.628
更新日期:1991-11-01 00:00:00