Abstract：
Objectives:To determine whether V H family usage in B-cell lymphoproliferative disorders can be deduced from polymerase chain reaction (PCR) product-length information obtained through the BIOMED-2 (Invivoscribe, San Diego, CA) clonality assay. Methods:We develop an algorithm that uses the sizing information of the BIOMED-2 immunoglobulin heavy chain (IGH) clonality assay to deduce V H family usage. PCR with family-specific primers on 51 clinical samples containing 54 rearranged alleles were used to validate the algorithm. Results:The clonal PCR products in different framework reactions contain the same NDN segment (because they are from the same allele). Subtracting the size of the framework III product from the size of the framework I and II products yields the relative position of the framework primer binding sites for the V H segment used. The V H family can be assigned with these relative positions because they are V H family specific in the BIOMED-2 assay. The V H family assigned by the algorithm was concordant with family-specific PCR results for 49 (96%) of the 51 specimens. Conclusions:We have developed an algorithm that can correctly assign V H family usage when all three BIOMED-2 framework reactions produced clonal products. Given the wide adoption of BIOMED-2 assay, the algorithm can facilitate collection of IGH V H usage data without additional cost to the laboratories.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
McDonald TJ,Kuo L,Kuo FCdoi
10.1093/ajcp/aqx007subject
Has Abstractpub_date
2017-06-01 00:00:00pages
549-556issue
6eissn
0002-9173issn
1943-7722pii
3793279journal_volume
147pub_type
杂志文章abstract：:Absolute lymphocytosis after nonsurgical trauma was investigated in three patient groups at an acute-care tertiary referral hospital. The first group, with mild-to-moderate trauma, consisted of 64 patients who survived knife wounds to the chest and abdomen. Thirteen of the 64 patients had admission lymphocyte counts g...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/86.4.480
更新日期：1986-10-01 00:00:00
abstract：:We compared a rapid, point-of-care multimarker protocol with a single and serial troponin I (TnI)-only protocol in 5,244 patients admitted to the emergency department with chest pain. The diagnosis of acute myocardial infarction (AMI) was based on a doubling myoglobin level accompanied by at least a 50% increase in th...
journal_title：American journal of clinical pathology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1309/9GGNMURLJWJD88W3
更新日期：2008-05-01 00:00:00
abstract：:A 75-year-old man with Staphylococcus aureus endocarditis in whom acute diffuse proliferative glomerulonephritis developed is described. The light- and electron-microscopic changes of the glomeruli in this case were identical to those of acute poststreptococcal glomerulonephritis. Immunofluorescence revealed depositio...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/70.5.832
更新日期：1978-11-01 00:00:00
abstract：:Cytopathologic smears and/or imprints of human adrenal cortex (9 cases) and its disorders were examined, including adrenocortical nodule (3 cases), adrenocortical adenoma (23 cases), carcinoma (8 cases), and renal cell carcinoma (6 cases). Immunocytochemistry directed against 3 beta-hydroxysteroid dehydrogenase and ad...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/104.2.161
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVES:To explore the survival and definition of stage IA mixed endometrial carcinoma. METHODS:From June 1, 2010, to June 1, 2017, cases with stage IA endometrial cancer were included in this study. The survival outcomes were compared among patients with endometrioid (group A), nonendometrioid (group B), and mixed...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqz083
更新日期：2019-10-07 00:00:00
abstract：:A case of septicemia caused by Pseudomonas paucimobilis is reported. This represents the first definitive case of disease produced by this yellow-pigmented, nonfermentative bacillus. ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/72.5.882
更新日期：1979-11-01 00:00:00
abstract：:A computer software package written in Dibol for a PDP 8/E computer is described that is designed for the needs of the cytology department of a moderate-sized private medical laboratory. Computerized accessioning of cases, report preparation and printing, historical filing, activity summary reports, and direct patient...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/71.4.421
更新日期：1979-04-01 00:00:00
abstract：:The aim of the present study was to determine whether micromorphometric features of positive sentinel lymph nodes (SLNs) from patients with melanoma are useful for predicting further nodal involvement in completion lymph node dissection (CLND) specimens. Of 986 patients with melanoma undergoing SLN biopsy between Marc...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/TDWJ-TR15-TDM1-TG7Q
更新日期：2004-10-01 00:00:00
abstract：:Although the relationship between pathologic features and clinical outcome is well established in adult adrenocortical neoplasms, the prognostic value of these features in pediatric adrenocortical neoplasms (PACN) is unclear. In a series of PACNs from 54 Brazilian children, the authors retrospectively investigated the...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/101.5.625
更新日期：1994-05-01 00:00:00
abstract：:An atypical form of macrocytosis termed volumetric macrocytosis is described. In contrast to the macrocyte associated with megaloblastic anemia and the pseudomacrocyte caused by viscoelastic defects, the volumetric macrocyte is characterized by an increased mean corpuscular volume and a normal cell diameter. The volum...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/85.3.337
更新日期：1986-03-01 00:00:00
abstract：:The role of viruses in several renal diseases is not documented clearly. The authors attempted to localize envelope glycoproteins of Hantaan virus in biopsy specimens from patients with Korean hemorrhagic fever (KHF) as evidence of direct viral invasion of renal tissues. The authors studied sequential sections of kidn...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/100.4.398
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVES:The incidence of lymphoproliferative disorders (LPDs) is increasing in sub-Saharan Africa (SSA) due to population growth, aging, and human immunodeficiency virus (HIV). Despite significant burden, resources for diagnosis and treatment of LPDs are limited, with little infrastructure to deliver modern patholog...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/aqw118
更新日期：2016-10-01 00:00:00
abstract：:Blood glucose results for 2,601 specimens obtained at multiphasic health examination, divided into three age groups, were analyzed using probability plot paper. Two straight lines could be fitted to each distribution. The assumption was made that the lower lines correspond to "normal-healthy" persons, whereas the uppe...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.2.211
更新日期：1981-08-01 00:00:00
abstract：:The characteristics of the normal adult thymus in both sexes were determined in 50 cases of accidental death by a simplified quantitative histologic technic. A table of normal values derived from these findings was used for making comparisons with thymus from autopsies of 50 additional patients suffering terminal illn...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/76.5.657
更新日期：1981-11-01 00:00:00
abstract：:The Laboratory Proficiency Testing Program has been in effect for 20 years. During the past 6 years, samples were distributed for screening, investigation, and identification of hemoglobinopathies to test laboratory proficiency. Six samples for hemoglobin (Hb) S screening were distributed to from 37 to 163 laboratorie...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.5.567
更新日期：1997-05-01 00:00:00
abstract：:Acute exacerbations of idiopathic pulmonary fibrosis/cryptogenic fibrosing alveolitis (IPF/CFA) are rare and typically terminal events, but their relationship to underlying patterns of idiopathic interstitial pneumonias is unknown. We reviewed autopsy material from patients who died of diffuse alveolar damage in the c...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/UVAR-MDY8-FE9F-JDKU
更新日期：2003-05-01 00:00:00
abstract：:Patients using anticoagulation point-of-care (POC) monitors are advised to periodically test these systems against laboratory methods to monitor performance. The international normalized ratio (INR), however, can vary between test systems owing to different instrument-reagent combinations. In a randomized study evalua...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：
更新日期：2005-02-01 00:00:00
abstract：:The authors report here a case of lymphoepithelial cystic lesion (LECL) of unknown origin in the mediastinum, which is closely related to a signet-ring cell adenocarcinoma. A 73-year-old man presented with a mass as revealed on a chest x-ray. During surgical operation, a solid, well-circumscribed and encapsulated 9 X ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/92.6.808
更新日期：1989-12-01 00:00:00
abstract：:Overexpression and amplification of the HER-2 oncogene in patients with breast cancer has correlated with early onset of metastasis, resistance to hormonal therapy and some forms of chemotherapy, and shortened survival. Therefore, evaluation of this putative prognostic or predictive factor seems critical. Because diff...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/0F58-0GRX-FK4R-A6VA
更新日期：2000-05-01 00:00:00
abstract：:A nodule of the liver was incidentally noted during left colectomy for a large villous adenoma in a 74-year-old man. Microscopic examination disclosed a granuloma of the liver containing numerous Enterobius vermicularis ova and a cross-section of the nematode. This is the fourth reported case of hepatic oxyuriasis and...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.1.97
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVES:To evaluate the clinical utility of and demand for the creatine kinase (CK)-MB assay. METHODS:We examined the number of CK-MB tests from 2007 through 2013 while we progressively deemphasized their use. We first removed CK-MB from the acute coronary syndrome (ACS) panel and then from the main menu and observ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPBIK3G4BWEJKO
更新日期：2014-03-01 00:00:00
abstract：:The prognostic value of DNA ploidy and proliferative index (PI) are well established in many cancers, but their significance in childhood rhabdomyosarcoma (RMS) is unclear. We studied the DNA content and PI of 45 cases of childhood RMS obtained retrospectively. DNA histograms were hyperdiploid in 30 cases (67%), diplo...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/3DD8-0UC4-DDJ0-JMT2
更新日期：2004-03-01 00:00:00
abstract：Objectives:Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of hematolymphoid proliferations arising in the context of chronic immunosuppression. The common and indolent B-cell lymphomas, including extranodal marginal zone lymphomas (ENMZLs) of mucosa-associated lymphoid tissue (MALT), are...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1093/ajcp/aqx134
更新日期：2017-12-20 00:00:00
abstract：:Two unusual cases of acute lymphoblastic leukemia-hand mirror variant (ALL-HMV) are presented. One patient demonstrated a mixed immunophenotype with HLA-DR, My7, transferrin receptor surface markers, and terminal deoxynucleotidyl transferase positivity. To the authors' knowledge, this is the first ALL-HMV reported wit...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/91.4.410
更新日期：1989-04-01 00:00:00
abstract：:Nineteen patients with acute nonlymphocytic leukemia were found to have a pericentric inversion of chromosome 16. All were classified as having acute myelomonocytic leukemia (M4) according to French-American-British Cooperative Group (FAB) criteria. Consistent abnormalities of marrow eosinophils were present. In 15 of...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/81.6.733
更新日期：1984-06-01 00:00:00
abstract：:Blastomycosis generally results from inhalation of Blastomyces dermatitidis conidia following exposure to contaminated soil in an endemic area. Primary infections commonly involve the lungs, although secondary dissemination to other body sites may occur. We describe 2 cases of osseous blastomycosis in people living ou...
journal_title：American journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1309/JEJ0-3N98-C3G8-21DE
更新日期：2002-10-01 00:00:00
abstract：:While plasmacytosis is a common occurrence in early post-bone marrow transplantation biopsy specimens, the significance of plasma cells in such specimens from patients with multiple myeloma (MM) is unknown. We attempted to retrospectively determine, by morphologic assessment of plasma cell percentage, immunohistologic...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/107.6.643
更新日期：1997-06-01 00:00:00
abstract：:To investigate the specificity of terminal deoxynucleotidyl transferase (TdT) as a marker for lymphoblastic lymphoma in the non-Hodgkin's lymphomas, the authors assayed malignant cells from 142 patients with non-Hodgkin's malignant lymphoma, as well as cells from normal lymphoid tissue controls. Neoplastic cells from ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1093/ajcp/80.5.655
更新日期：1983-11-01 00:00:00
abstract：:Fluorescence in situ hybridization (FISH) and immunohistochemical analysis for assessment of HER-2 status in breast carcinomas are discordant in a significant proportion of cases with equivocal (2+) immunostaining. To evaluate the role of intratumoral heterogeneity and degree of amplification, we performed additional ...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/J9VX-ABUG-KC4Y-07DL
更新日期：2005-08-01 00:00:00
abstract：OBJECTIVES:To support our Lean culture of continuous improvement, we implemented a daily management system designed so critical metrics of operational success were the focus of local teams to drive improvements. METHODS:We innovated a standardized visual daily management board composed of metric categories of Quality,...
journal_title：American journal of clinical pathology
pub_type： 杂志文章
doi：10.1309/AJCPLQYMOFWU31CK
更新日期：2015-07-01 00:00:00
