Abstract:
:Same-day turnaround of pathology specimens is desirable in this era of managed care, and rapid microwave tissue processing produces histologic features of a quality equivalent to overnight processing. We studied whether microwave-assisted rapid tissue processing adversely affects the quality of immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10 nonneoplastic) from our routine surgical pathology workload. Paired large tissue blocks were made from each specimen type, one for microwave-assisted rapid processing and one for conventional processing. Two microarrays of 60 punches each were made from the donor blocks. The microarray blocks were examined for intensity and extent of staining by 44 commonly used antibodies. Slides were reviewed independently by 2 pathologists blinded to the type of processing used. In 5,280 tissue punches examined, we found a high degree of concordance in quality, as measured by intensity and extent of immunohistochemical staining, between microwave and routinely processed tissues. Our study demonstrates that quality of immunohistochemical staining is similar between rapid microwave and conventional processing. The potential need for immunohistochemical analysis is not a contraindication for microwave-assisted rapid tissue processing.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Emerson LL,Tripp SR,Baird BC,Layfield LJ,Rohr LRdoi
10.1309/GN6Q-CBML-LEAT-LK2Msubject
Has Abstractpub_date
2006-02-01 00:00:00pages
176-83issue
2eissn
0002-9173issn
1943-7722pii
GN6QCBMLLEATLK2Mjournal_volume
125pub_type
杂志文章abstract::We performed a retrospective clinicopathologic study on sequential biopsy specimens from 90 patients with Philadelphia chromosome-positive chronic myelogenous leukemia to study therapy-specific effects of busulfan (28 patients), hydroxyurea (32 patients), and interferon-alfa (IFN-alfa; 30 patients). Bone marrow specim...
journal_title:American journal of clinical pathology
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doi:10.1309/XMGX-7HQ8-7PLU-LQ9M
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journal_title:American journal of clinical pathology
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journal_title:American journal of clinical pathology
pub_type: 杂志文章,多中心研究
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journal_title:American journal of clinical pathology
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更新日期:1988-09-01 00:00:00
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journal_title:American journal of clinical pathology
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pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:American journal of clinical pathology
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pub_type: 杂志文章
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更新日期:2017-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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journal_title:American journal of clinical pathology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00