Cytogenetic aberrations and immunoglobulin VH gene mutations in clinically benign CD5- monoclonal B-cell lymphocytosis.

Abstract:

:The finding of monoclonal B-cell lymphocytosis (MBL) raises questions on the nature of clonal cell expansion and its risk of progression. We identified and characterized 7 cases of clinically benign clonal B-cell lymphocytosis. The clonal lymphocytes were clearly of CD5- and non-chronic lymphocytic leukemia (CLL) phenotype. All cases had mild to moderate absolute lymphocytosis. The clonal population accounted for 95% to 99% of B cells. For a follow-up period of 4 to 16 years, clonal lymphocytosis was persistent but virtually not progressing. Patients' conditions remained clinically stable and asymptomatic. The clonal populations had somatic hypermutations of the VH gene in 6 cases, indicating a germinal center or post-germinal center B-lymphocyte origin. Clonal cytogenetic aberrations were found in 5 of 6 cases, with 2 clones bearing isochromosome 17q that resulted in loss of p53 and 2 other clones with 7q abnormalities. By the presence of absolute lymphocytosis, this series differs from MBL cases identified by sensitive flow cytometry in normal populations. The phenotypic profiles are distinct from that of benign CLL. We suggest these CD5-B-cell lymphocytosis cases may represent an intermediate condition between covert clonal expansions and overt malignancy.

journal_name

Am J Clin Pathol

authors

Amato D,Oscier DG,Davis Z,Mould S,Zheng J,Kolomietz E,Wang C

doi

10.1309/WB56ET6AGM44TM5T

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

333-8

issue

2

eissn

0002-9173

issn

1943-7722

pii

Y3030GL4T5841181

journal_volume

128

pub_type

杂志文章
  • The LAST guidelines in clinical practice: implementing recommendations for p16 use.

    abstract:OBJECTIVES:To determine the impact of implementing p16 Lower Anogenital Squamous Terminology Standardization Project for HPV-Associated Lesions (LAST) guidelines, we compared p16 use and follow-up data before and after implementation of the guidelines. METHODS:We reviewed all cervical biopsy specimens diagnosed by two...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPUXLP7XD8OQYY

    authors: Clinton LK,Miyazaki K,Ayabe A,Davis J,Tauchi-Nishi P,Shimizu D

    更新日期:2015-12-01 00:00:00

  • Changes in Mean Corpuscular Volume and RBC Distribution Width Predict Erythrocyte Engraftment Following ABO-Incompatible Hematopoietic Stem Cell Transplantation.

    abstract:OBJECTIVES:The purpose of this study was to identify laboratory parameters representing erythrocyte engraftment to be used as an indicator to change the recipient to donor ABO group and Rh type following an ABO-incompatible hematopoietic stem cell transplant (HSCT). Studies have shown that ABO incompatibility does not ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa006

    authors: Sanford K,Case N,Blake B,Michaud T,McPherson RA,Roseff SD

    更新日期:2020-05-05 00:00:00

  • Mucosa-associated lymphoid tissue of the thymus hyperplasia vs lymphoma.

    abstract::In the thymus, the relationship between lymphofollicular hyperplasia and mucosa-associated lymphoid tissue (MALT)-type lymphoma is uncertain. We analyzed 14 cases with a diagnosis of thymic follicular hyperplasia in patients with connective tissue disease (n = 2), myasthenia gravis (n = 11), or both (n = 1). In 11 cas...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/G3AC-P7K8-2JNK-8C3U

    authors: Parrens M,Dubus P,Danjoux M,Jougon J,Brousset P,Velly JF,de Mascarel A,Merlio JP

    更新日期:2002-01-01 00:00:00

  • The effect of media and temperature on the storage of Chlamydia trachomatis.

    abstract::Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.237

    authors: Aarnaes SL,Peterson EM,De La Maza LM

    更新日期:1984-02-01 00:00:00

  • Evaluation of volume and total nucleated cell count as cord blood selection parameters: a receiver operating characteristic curve modeling approach.

    abstract::The objective of the study was to evaluate the current standard practice of using volume and total nucleated cell (TNC) count for the selection of cord blood (CB) units for cryopreservation and further transplantation. Data on 794 CB units whose CD34+ cell content was determined by flow cytometry were analyzed by usin...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPFB6EXO7BJVLR

    authors: Jaime-Pérez JC,Monreal-Robles R,Rodríguez-Romo LN,Mancías-Guerra C,Herrera-Garza JL,Gómez-Almaguer D

    更新日期:2011-11-01 00:00:00

  • Comparison of next-generation sequencing and mutation-specific platforms in clinical practice.

    abstract:OBJECTIVES:To compare next-generation sequencing (NGS) platforms with mutation-specific analysis platforms in a clinical setting, in terms of sensitivity, mutation specificity, costs, capacity, and ease of use. METHODS:We analyzed 25 formalin-fixed, paraffin-embedded lung cancer samples of different size and tumor per...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP40XETVYAMJPY

    authors: Hinrichs JW,van Blokland WT,Moons MJ,Radersma RD,Radersma-van Loon JH,de Voijs CM,Rappel SB,Koudijs MJ,Besselink NJ,Willems SM,de Weger RA

    更新日期:2015-04-01 00:00:00

  • Spurious hemoglobin Barts caused by bilirubin: a common interference mimicking an uncommon hemoglobinopathy.

    abstract::High-performance liquid chromatography (HPLC) is replacing electrophoresis for identification of hemoglobin variants. Our objective was to identify unknown tall peaks with elution times and shapes of hemoglobin Barts found on hemoglobin chromatograms that could not be confirmed by alkaline and acid gel electrophoresis...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/73WJ-V2E0-RTVQ-H0BB

    authors: Howanitz PJ,Kozarski TB,Howanitz JH,Chauhan YS

    更新日期:2006-04-01 00:00:00

  • Safety and cost-containment data that advocate abbreviated pretransfusion testing.

    abstract::Abbreviated pretransfusion testing, although permitted by American Association of Blood Banks Standards for unimmunized patients, is not widely practiced. Concerns remain about optimal antibody screening methods, antibodies missed by deleting the antiglobulin crossmatch, and cost-effectiveness. The authors prospective...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.4.428

    authors: Cordle DG,Strauss RG,Snyder EL,Floss AM

    更新日期:1990-10-01 00:00:00

  • Concurrent antiglomerular basement membrane antibody and immune complex mediated glomerulonephritis.

    abstract::A teenage male, with Goodpasture's syndrome and serum antiglomerular basement membrane (anti-GBM) antibodies, had a focal proliferative glomerulonephritis with crescents. Immunofluorescence microscopy of his glomeruli using anti-IgG antibodies demonstrated both intense linear GBM staining, and granular subepithelial s...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.3.381

    authors: Jennette JC,Lamanna RW,Burnette JP,Wilkman AS,Iskander SS

    更新日期:1982-09-01 00:00:00

  • Analysis of intestinal lymphocyte subpopulations in patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex.

    abstract::Lymphocyte subpopulations in the intestinal tissues of seven patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) were studied by immunohistologic technics at two different locations, the small bowel and the rectum. Intraepithelial and lamina propria lymphocyte subsets stained with mon...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.3.356

    authors: Ellakany S,Whiteside TL,Schade RR,van Thiel DH

    更新日期:1987-03-01 00:00:00

  • Antithrombin III and Factor VIII in patients with neoplasms.

    abstract::Possible increased activation of the coagulation pathway was measured in a group of patients with neoplastic diseases. In addition to standard tests, the thromboplastin generation test, thrombin generation test and immunologic and coagulant activities of both Factor VIII and antithrombin III were utilized in the evalu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/68.2.258

    authors: Losito R,Beaudry P,Valderrama JC,Cousineau L,Longpré B

    更新日期:1977-08-01 00:00:00

  • Squamous metaplasia of the prostate. An immunohistochemical study.

    abstract::Immunoperoxidase strains for prostate-specific antigen (PSA), prostatic acid phosphatase (PAcP), epithelial membrane antigen (EMA), and cytokeratins (MAK 6 and CK-KES) were performed on 1 case of squamous cell carcinoma of the prostate and on 13 cases of squamous metaplasia of prostatic epithelium in an effort to demo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/90.5.597

    authors: Lager DJ,Goeken JA,Kemp JD,Robinson RA

    更新日期:1988-11-01 00:00:00

  • Hand-mirror cell leukemia. Report of nine cases and a review of the literature.

    abstract::In order to determine the significance of 40% or more leukemic cells in hand-mirror configuration in the bone marrow (hand-mirror cell leukemia), eight of 34 cases of acute lymphoblastic leukemia and one of 17 cases of acute myeloblastic leukemia were collected from a six-year period. Five of the eight patients who ha...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/74.5.651

    authors: Glassy EF,Sun NC,Okun DB

    更新日期:1980-11-01 00:00:00

  • Detection of chlamydial cervicitis by Papanicolaou stained smears and culture.

    abstract::The ability of the cervical Papanicolaou (Pap) smear to detect cervicitis associated with Chlamydia trachomatis was investigated. Cultures and cervical cytology samples were obtained from 487 women seen at the Mayo Clinic Department of Obstetrics and Gynecology and at the Sexually Transmitted Disease clinic of the Olm...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.4.421

    authors: Dorman SA,Danos LM,Wilson DJ,Noller KL,Malkasian GD,Goellner JR,Smith TF

    更新日期:1983-04-01 00:00:00

  • Nocardia asteroides bacteremia in an immunosuppressed renal-transplant patient.

    abstract::A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.4.537

    authors: Ruebush TK 2nd,Goodman JS

    更新日期:1975-10-01 00:00:00

  • Detection of allergic disease in adenoid tissue.

    abstract::Allergic adenoiditis is defined as the presence of numerous brightly fluorescent IgE mast cells (FIEMC) demonstrated in formalin-fixed adenoid tissue by the trypsin-immunofluorescent method. A prospective study of 606 routinely accessioned cases seen during a 39-month period revealed 98 positive cases. Correlation wit...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.170

    authors: Loesel LS

    更新日期:1984-02-01 00:00:00

  • Pseudo-Pelger-Huët anomaly induced by medications: a clinicopathologic study in comparison with myelodysplastic syndrome-related pseudo-Pelger-Huët anomaly.

    abstract::Pseudo-Pelger-Huët anomaly (PPHA) has been documented in association with transplant medications and other drugs. This iatrogenic neutrophilic dysplasia is reversible with cessation or adjustment of medications but is frequently confused with myelodysplastic syndrome (MDS) based on the conventional concept that PPHA i...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPVFY95MAOBKRS

    authors: Wang E,Boswell E,Siddiqi I,Lu CM,Sebastian S,Rehder C,Huang Q

    更新日期:2011-02-01 00:00:00

  • Presence of t(8;21)(q22;q22) in myeloperoxidase-positive, myeloid surface antigen-negative acute myeloid leukemia.

    abstract::Although acute myeloid leukemias (AMLs) cytochemically negative for myeloperoxidase are now well recognized, myeloid surface antigen-negative AMLs are rare. The morphologic, cytochemical, immunologic, and cytogenetic or molecular features of such cases are described in four adults aged 19 to 60 years. All had AML with...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/107.1.68

    authors: Arber DA,Glackin C,Lowe G,Medeiros LJ,Slovak ML

    更新日期:1997-01-01 00:00:00

  • Small cell carcinoma of the endometrium in a 23-year-old woman: light microscopic and ultrastructural study.

    abstract::The case of a 23-year-old woman with a primary small cell carcinoma of the endometrium is reported. The light microscopic features were similar to those of small cell carcinoma of the lung and those of the uterine cervix. Electron microscopy revealed cytoplasmic neurosecretory type granules and indistinct cell junctio...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.1.98

    authors: Kumar NB

    更新日期:1984-01-01 00:00:00

  • Assessment of a critical limit protocol for point-of-care glucose testing.

    abstract::A critical limit protocol requiring that all point of care glucose meter readings > 22.2 mmol/L (400 mg/dL) and < 2.2 mmol/L (40 mg/dL) be immediately confirmed by the laboratory was assessed. A total of 193 (2%) of 9,523 glucose meter determinations (63 patients) were > 22.2 or < 2.2 mmol/L. One hundred twenty-two (6...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.3.390

    authors: Lum G

    更新日期:1996-09-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.557

    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Globin chain synthesis in Hb J Baltimore-beta (+)-thalassemia.

    abstract::An American black family in whom hemoglobin J Baltimore and beta (+)-thalassemia genes coexisted is described. The proposita is a 23-year-old woman with a hemoglobin (Hb) level of 11.5 g/dl, microcytic, hypochromic indices, increased values of Hbs A2 and F, and alpha/non-alpha synthetic ratio of 1.52. Hbs A and J Balt...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.6.843

    authors: Ballas SK,Atwater J,Theriault C,Kim HC,Propst M

    更新日期:1981-06-01 00:00:00

  • Modification of solid phase red cell adherence assay for the detection of platelet antibodies in patients with thrombocytopenia.

    abstract::Platelet refractoriness is caused by HLA antibodies and platelet-specific antibodies. Current methods used to detect antiplatelet antibodies have limitations. Solid phase red cell adherence (SPRCA) lacks sensitivity and requires a second assay using chloroquine-treated intact platelets to specify the response due to a...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/1QWTQFMF0Q9JEAGR

    authors: Vongchan P,Nawarawong W,Linhardt RJ

    更新日期:2008-09-01 00:00:00

  • Potential use of monoclonal antibodies in the diagnostic distinction of gynecomastia from breast carcinoma in men.

    abstract::Immunohistochemical (IHC) assays using the monoclonal antibodies (MoAbs) B72.3 and B6.2, recognizing two distinct and independently expressed breast tumor-associated antigens (BTAAs), recently have been shown to significantly improve the accuracy of cytodiagnosis of breast nodules by fine-needle aspiration (FNA). To e...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.2.233

    authors: Mottolese M,Bigotti G,Coli A,Vitucci C,Natali PG

    更新日期:1991-08-01 00:00:00

  • Intratumoral genetic heterogeneity in Barrett adenocarcinoma.

    abstract::In 10 cases of Barrett adenocarcinoma, samples from 8 tumor areas (including superficial and deep from peripheral and central areas) and a regional lymph node metastasis were studied for amplification of c-myc, c-erbB-2, and EGFR. We analyzed loss of heterozygosity (LOH) at 3 loci (APC, MCC, and RB) and 2 anonymous mi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/6XY8-FMM5-VVCJ-T3N0

    authors: Owonikoko T,Rees M,Gabbert HE,Sarbia M

    更新日期:2002-04-01 00:00:00

  • The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and β-thalassemia screening.

    abstract::Cell counter-based formulas have been used in the differential diagnosis of microcytic anemia. The measurement of RBC subpopulations is now available on the Sysmex XE 5000 analyzer (Sysmex, Kobe, Japan). We describe the new formulas: % microcytic - % hypochromic; and % microcytic - % hypochromic - red cell distributio...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPJRH1I0XTNFGA

    authors: Urrechaga E,Borque L,Escanero JF

    更新日期:2011-03-01 00:00:00

  • Adamantinoma of the pretibial soft tissue. Clinicopathologic features, differential diagnosis, and possible relationship to intraosseous disease.

    abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.1.108

    authors: Mills SE,Rosai J

    更新日期:1985-01-01 00:00:00

  • Serum alpha-fetoprotein. A prognostic indicator of liver-cell necrosis and regeneration following experimental injury by galactosamine in rats.

    abstract::The serum concentrations of alpha-fetoprotein of rats following experimental galactosamine-induced liver-cell necrosis accurately reflect the severity of preceding liver-cell damage as determined by elevation of serum transaminases. There is a very close correlation between the highest SGOT or SGPT serum activity foun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.5.847

    authors: Sell S,Stillman D,Gochman N

    更新日期:1976-11-01 00:00:00

  • Detection of circulating T-activating enzyme in the serum of a patient having hemolytic-uremic syndrome and disseminated intravascular coagulation.

    abstract::A three-and-a-half-year old boy suffering from streptococcal pneumonia developed hemolytic-uremic syndrome and disseminated intravascular coagulation (DIC). His red blood cells (RBC) were shown to be T- and Tk-activated; serial testing showed his mature RBCs as well as neocytes remained T-activated at 40 days. Anti-T ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.2.244

    authors: Beattie KM,Lewis PE,Briski JE,Strauch BM

    更新日期:1985-08-01 00:00:00

  • Analysis of heparinization methods during hemodialysis.

    abstract::The ideal method of heparinization should achieve therapeutic concentrations (0.2-0.5 IU/mL) in the artificial kidney and the least possible amount of heparin in the patient. Total heparinization using a bolus dose (8400 IU) followed by continuous infusion of heparin (20 IU/min), initially showed 1.4-2.4 IU/mL in the ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.3.317

    authors: Talstad I,Kjelby K

    更新日期:1985-09-01 00:00:00