Evaluation of three commercial enzyme immunoassays for toxoplasma and cytomegalovirus antibodies.

Abstract:

:Three commercially available enzyme immunoassays (EIAs) were evaluated for specificity and sensitivity compared to immunofluorescent assays (IFAs) for detection of IgG and IgM antibodies to Toxoplasma gondii (Toxo) and Cytomegalovirus (CMV). A panel of 78 sera were tested, including specimens known to contain Toxo IgG or IgM, CMV IgM or IgM, antinuclear antibodies (ANA), antimitochondrial antibodies (AMA), and rheumatoid factor (RF). Linear correlation analysis comparing EIA and IFA results showed that the statistical relationship between the two assay methods was relatively weak (correlation coefficients ranging from 0.33 to 0.86). Qualitative correlation showed that the EIA methods agreed with IFA results in most cases. One manufacturer's products (Diamedix Corp.) gave consistently better sensitivity, specificity, and intra-assay reproducibility values than the other two systems (Clinical Sciences, Inc., and Whittaker Bioproducts). ANA, AMA, and RF did not appear to interfere with any EIAs.

journal_name

Am J Clin Pathol

authors

Van Enk RA,James KK,Thompson KD

doi

10.1093/ajcp/95.3.428

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

428-34

issue

3

eissn

0002-9173

issn

1943-7722

journal_volume

95

pub_type

杂志文章
  • Fine-needle aspiration cytologic diagnosis of Kikuchi's lymphadenitis. A report of 27 cases.

    abstract::Kikuchi's lymphadenitis is a self-limiting condition typically affecting young patients. Surgical biopsy is unnecessary if a firm diagnosis can be rendered by fine-needle aspiration cytology (FNAC). The authors report the FNAC findings for 27 cases, including 24 female and 3 male patients aged 12 to 43 years. Histolog...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/102.4.454

    authors: Tsang WY,Chan JK

    更新日期:1994-10-01 00:00:00

  • Immunohistochemical vs molecular biology methods. Complementary techniques for effective screening of p53 alterations in head and neck cancer.

    abstract::The purpose of this study was to correlate p53 gene alterations and their expression in 85 head and neck squamous cell carcinomas. Genomic p53 was amplified with the polymerase chain reaction (PCR) from formalin-fixed, paraffin-embedded tissues. Exons 5 through 8 were screened for mutations by means of non-isotopic si...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/107.1.7

    authors: Calzolari A,Chiarelli I,Bianchi S,Messerini L,Gallo O,Porfirio B,Mattiuz PL

    更新日期:1997-01-01 00:00:00

  • Comparison of eight kits for the diagnosis of pregnancy.

    abstract::The results obtained with eight pregnancy kits on 735 urines were compared. Overall accuracies were similar and fairly high (93.4 to 95.37%), although only 78.28% of all pregnancies were simultaneously detected by all eight kits. It is suggested that the different performances of each kit could be accounted for not on...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.4.452

    authors: Porres JM,D'Ambra C,Lord D,Garrity F

    更新日期:1975-10-01 00:00:00

  • Misleading hepatitis C serology following administration of intravenous immunoglobulin.

    abstract::Intravenous immunoglobulin (IVIG) is a blood derivative that is being evaluated for the prevention and treatment of numerous disorders. Recent studies have suggested beneficial effects from the prophylactic administration of IVIG to very-low-birth-weight neonates. A disadvantage of such therapy is misleading infectiou...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.3.327

    authors: Nixon RR,Smith SA,Johnson RL,Pillers DA

    更新日期:1994-03-01 00:00:00

  • The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumors.

    abstract::Vimentin has been regarded as the intermediate filament characteristic of normal and neoplastic mesenchymal cells and keratins as typical of epithelial cells and the neoplasms derived from them. However, many epithelial cells in tissue culture or in effusion, as well as cells in some solid epithelial neoplasms such as...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/88.3.286

    authors: Azumi N,Battifora H

    更新日期:1987-09-01 00:00:00

  • Cancer tracking: Clinician compliance and program outcome.

    abstract:OBJECTIVES:Certain laboratory tests are critical for patient care and must be communicated rapidly. Professional societies have developed guidelines for reporting of "critical values" and significant or unexpected surgical pathology findings. Urgent diagnoses requiring rapid reporting include new or unexpected diagnose...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPJHFVORM48IZL

    authors: Layfield LJ,Schnabel JL

    更新日期:2014-12-01 00:00:00

  • Image cytometric measurement of nuclear proliferation markers (MIB-1, PCNA) in astrocytomas. Prognostic significance.

    abstract::Proliferative activity in astrocytomas, measured by image cytometry, was related to prognosis. Fifty-eight astrocytic neoplasms (grade 1 or 2, 11; grade 3, 31; glioblastoma multiforme, 16) were immunostained for Ki-67 (MIB-1; 1:50) and proliferating cell nuclear antigen (PCNA; prediluted). Proliferative activity (nucl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.1.69

    authors: Kirkegaard LJ,DeRose PB,Yao B,Cohen C

    更新日期:1998-01-01 00:00:00

  • Comparison of bcr-abl protein expression and Philadelphia chromosome analyses in chronic myelogenous leukemia patients.

    abstract::The Philadelphia chromosome (Ph) is found in most chronic myelogenous leukemia (CML) patients. The bcr-abl oncoprotein (P210 or P185), the product of the fused bcr-abl gene produced by the Ph, is known to be the major factor in initiation and maintenance of the leukemic state in these types of leukemias. The authors h...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.4.442

    authors: Guo JQ,Lian J,Glassman A,Talpaz M,Kantarjian H,Deisseroth AB,Arlinghaus RB

    更新日期:1996-10-01 00:00:00

  • Evaluation of the Abbott CELL-DYN 4000 hematology analyzer.

    abstract::A new generation hematology analyzer, Abbott CELL-DYN 4000 (CD 4000), capable of providing 26 parameters, including fully automated reticulocyte, nucleated RBC, blast, band, and immature granulocyte, and variant lymphocyte counts, was evaluated by using the National Committee for Clinical Laboratory Standards H20-A pr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/L7LC-6KKJ-12G2-196W

    authors: Grimaldi E,Scopacasa F

    更新日期:2000-04-01 00:00:00

  • Laboratory testing for viral hepatitis.

    abstract::Worldwide, infection with hepatitis viruses remains a major clinical and public heath concern. The laboratory is an integral part of the diagnosis of these infections since many of the manifestations are nonspecific, and the various hepatitis viruses show overlapping symptomatology. In this article, we discuss the lab...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/MAUW-9059-DQA6-RMRJ

    authors: Harris KR,Dighe AS

    更新日期:2002-12-01 00:00:00

  • Fine-needle aspiration biopsy of the mediastinum. A multi-institutional analysis.

    abstract::The combined experience of four university medical centers with fine-needle aspiration biopsy (FNAB) of the mediastinum is reviewed. This series includes 189 cases, with 100 males and 89 females, 6 months to 86 years of age. The majority (71%) of diagnoses were neoplastic with the remainder equally distributed between...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,多中心研究

    doi:10.1093/ajcp/105.2.168

    authors: Powers CN,Silverman JF,Geisinger KR,Frable WJ

    更新日期:1996-02-01 00:00:00

  • Rapid detection of Klebsiella pneumoniae carbapenemase genes in enterobacteriaceae directly from blood culture bottles by real-time PCR.

    abstract::Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic in New York City hospitals and have been associated with serious infections globally. A real-time polymerase chain reaction (RT-PCR) assay was developed to detect carbapenem resistance attributable to KPC from blood culture bottles posi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP9SNHJG2QGLWU

    authors: Francis RO,Wu F,Della-Latta P,Shi J,Whittier S

    更新日期:2012-04-01 00:00:00

  • Practical use of computerized hospital information systems to improve blood transfusion.

    abstract::Data collection can be enhanced with the use of computers. Care must be taken, however, to ensure that the data collected have a purpose and meet either the quality assessment needs or business functions of the transfusion service. Required data elements are frequently in several data repositories and must be merged t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Butch SH

    更新日期:1997-04-01 00:00:00

  • Acetaminophen overdose with fetal demise.

    abstract::A case of fetal demise and maternal recovery after acetaminophen overdose is presented, to our knowledge the first reported. Fetal liver and maternal serum concentrations indicate overdose to be the cause of fetal death. Maternal disseminated intravascular coagulation (DIC) may have been related to maternal acetaminop...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.2.240

    authors: Haibach H,Akhter JE,Muscato MS,Cary PL,Hoffmann MF

    更新日期:1984-08-01 00:00:00

  • Altered expression, localization, and phosphorylation of epithelial junctional proteins in celiac disease.

    abstract::We aimed to study the expression and localization of the molecular components of enterocyte junctions in celiac disease together with the level of tyrosine phosphorylation, a phenomenon known to affect their cellular distribution and function, and to explore the influence of proinflammatory cytokines. Duodenal biopsy ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/DTYR-A91G-8R0K-TM8M

    authors: Ciccocioppo R,Finamore A,Ara C,Di Sabatino A,Mengheri E,Corazza GR

    更新日期:2006-04-01 00:00:00

  • True histiocytic lymphoma of the thymus. Report of a case and a study of the distribution of histiocytic cells in the fetal and adult thymus.

    abstract::The thymus was the site of a true histiocytic lymphoma. Immunohistochemical demonstration of lysozyme and alpha-1-antitrypsin in the tumor cells indicated the histiocytic nature of the tumor. Five fetal and five adult thymus glands were studied for the presence and distribution of true histiocytic cells. Histiocytes w...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.6.734

    authors: Szporn AH,Dikman S,Jagirdar J

    更新日期:1984-12-01 00:00:00

  • Management by objectives for the academic medical center.

    abstract::Management by Objectives (MBO) is results-oriented, participatory management, which is appropriate for the current era of keen competition for resources. MBO was installed in the author's institution in clinical laboratory divisions meeting prerequisites and fit the system to the local management environment. The resu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.6.738

    authors: Kost GJ

    更新日期:1986-12-01 00:00:00

  • Determination of terminal deoxynucleotidyl transferase on bone marrow smears by immunoperoxidase.

    abstract::Terminal deoxynucleotidyl transferase (TdT) is a marker of lymphoblastic disorders. Previously, the technique for determining TdT on smears was by indirect immunofluorescence. An immunoperoxidase procedure for detecting TdT on smears is reported. Results are in a comparable range with the indirect immunofluorescent te...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/77.2.174

    authors: Stass SA,Dean L,Peiper SC,Bollum FJ

    更新日期:1982-02-01 00:00:00

  • The use of monoclonal antibody 44-3A6 in cell blocks in the diagnosis of lung carcinoma, carcinomas metastatic to lung and pleura, and pleural malignant mesothelioma.

    abstract::Monoclonal antibody (MoAb) 44-3A6 recognizes a glandular differentiation-associated antigen and has been used to identify exocrine differentiation in pulmonary carcinomas. The authors assessed its value in the diagnosis of lung carcinomas metastatic to lung/pleura and pleural malignant mesothelioma (MM), using cell bl...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/95.3.322

    authors: Spagnolo DV,Whitaker D,Carrello S,Radosevich JA,Rosen ST,Gould VE

    更新日期:1991-03-01 00:00:00

  • Abdominal wall defect associated with persistent cloaca. The embryologic clues in autopsy.

    abstract::Three stillborn fetuses are reported in which an abdominal wall defect was associated with defects in the urogenital and anal region. Autopsy of these fetuses provided clues indicative of how and where the embryonic development went wrong. The malformation involved a disturbance of the cell deposition process, occurri...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.5.640

    authors: Hartwig NG,Steffelaar JW,Van de Kaa C,Schueler JA,Vermeij-Keers C

    更新日期:1991-11-01 00:00:00

  • Differential MUC 1 expression in normal and neoplastic human pancreatic tissue. An immunohistochemical study of 60 samples.

    abstract::Neoplastic transformation of epithelial cell sis commonly associated with altered synthesis of mucin glycoproteins. Few studies have been performed on the correlation between MUC 1 expression and pancreatic carcinoma using immunohistochemical methods. We compared the patterns of MUC 1 expression in normal pancreatic t...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/112.5.635

    authors: Monges GM,Mathoulin-Portier MP,Acres RB,Houvenaeghel GF,Giovannini MF,Seitz JF,Bardou VJ,Payan MJ,Olive D

    更新日期:1999-11-01 00:00:00

  • Leu-M1 antigen expression in advanced (tumor) stage mycosis fungoides.

    abstract::The authors describe two patients with clinically and histopathologically documented advanced (tumor) stage mycosis fungoides. In each case the large, pleomorphic neoplastic cells lacked the monoclonal antibody-defined cell surface antigens commonly associated with immature and mature T-cells, i.e., T11, Leu-1, T3, T4...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/86.1.25

    authors: Wieczorek R,Suhrland M,Ramsay D,Reed ML,Knowles DM 2nd

    更新日期:1986-07-01 00:00:00

  • Immunoalkaline phosphatase cytochemistry. Technical considerations of endogenous phosphatase activity.

    abstract::The authors have developed an immunoalkaline phosphatase method and have applied it with success to the study of blood cells. They have now observed that macrophages in tissues and in serous effusions may be nonspecifically stained when immunoalkaline phosphatase methods are used. A systematic study of this endogenous...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.4.476

    authors: Janckila AJ,Yam LT,Li CY

    更新日期:1985-10-01 00:00:00

  • Serum alpha-fetoprotein. A prognostic indicator of liver-cell necrosis and regeneration following experimental injury by galactosamine in rats.

    abstract::The serum concentrations of alpha-fetoprotein of rats following experimental galactosamine-induced liver-cell necrosis accurately reflect the severity of preceding liver-cell damage as determined by elevation of serum transaminases. There is a very close correlation between the highest SGOT or SGPT serum activity foun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/66.5.847

    authors: Sell S,Stillman D,Gochman N

    更新日期:1976-11-01 00:00:00

  • Myelodysplasia in the acquired immune deficiency syndrome.

    abstract::Certain new hematologic findings in eight homosexual or bisexual patients with the acquired immune deficiency syndrome (AIDS) are presented. All eight patients manifested a normochromic, normocytic anemia, and six of eight had granulocytopenia during their hospitalization. The other two had low-normal granulocyte coun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.2.144

    authors: Schneider DR,Picker LJ

    更新日期:1985-08-01 00:00:00

  • Measuring errors in surgical pathology in real-life practice: defining what does and does not matter.

    abstract::This review summarizes our experience using blinded review as a method to measure quality in surgical pathology. It details the specifics about how the review is performed, the weaknesses in the method, and then summarizes our results so far. These results suggest that error rates correlate with the individual patholo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/5KF89P63F4F6EUHB

    authors: Renshaw AA,Gould EW

    更新日期:2007-01-01 00:00:00

  • Immunoglobulin production in lymphomatoid granulomatosis and relation to other "benign" lymphoproliferative disorders.

    abstract::Immunoperoxidase technics were used to examine the immunoglobulin content of sections of pulmonary tissue from two typical cases of lymphomatoid granulomatosis and two cases of pneumonic processes initially diagnosed as lymphomatoid granulomatosis but representing different processes on review. Both "typical" cases an...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.1.41

    authors: Bender BL,Jaffe R

    更新日期:1980-01-01 00:00:00

  • Intratumoral DNA content variability. A study of non-small cell lung cancer.

    abstract::Twenty-eight resected primary non-small cell lung carcinomas were studied for intratumoral DNA variability by flow cytometry (FCM). Three separate tissue specimens from each resected tumor were divided equally for FCM analysis and histologic evaluation. FCM analysis also was performed on fine-needle aspirates (FNAs) f...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.3.311

    authors: Sara A,el-Naggar AK

    更新日期:1991-09-01 00:00:00

  • Determination of the oxygen dissociation curve and P50 of whole blood. An evaluation of the Hem-O-Scan and instrumentation of laboratory systems.

    abstract::The Travenol Hem-O-Scan and the Instrumentation Laboratory (IL) system for determination of the oxygen dissociation curve and P50 values have been evaluated. Using the Hem-O-Scan, an oxygen dissociation curve and P50 value may be obtained on 2 microliter of blood in 40 min, though the pH is not measured. In the IL sys...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.3.395

    authors: Nelson MG,Savage GA,Cooke PJ,Lappin TR

    更新日期:1981-03-01 00:00:00

  • Detection of allergic disease in adenoid tissue.

    abstract::Allergic adenoiditis is defined as the presence of numerous brightly fluorescent IgE mast cells (FIEMC) demonstrated in formalin-fixed adenoid tissue by the trypsin-immunofluorescent method. A prospective study of 606 routinely accessioned cases seen during a 39-month period revealed 98 positive cases. Correlation wit...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.170

    authors: Loesel LS

    更新日期:1984-02-01 00:00:00