Abstract:
:A new generation hematology analyzer, Abbott CELL-DYN 4000 (CD 4000), capable of providing 26 parameters, including fully automated reticulocyte, nucleated RBC, blast, band, and immature granulocyte, and variant lymphocyte counts, was evaluated by using the National Committee for Clinical Laboratory Standards H20-A protocol and compared with the Bayer-Technicon H-2 analyzer, which is used routinely in our laboratory. A lipid interference experiment and a sample aging study also were performed. Linearity, carryover, and precision were within the limits established by the manufacturer, and satisfactory agreement was found with the H-2 analyzer. The evaluation of leukocyte differential counts indicated an excellent correlation with the manual reference method for neutrophils and lymphocytes, a good correlation for monocytes and eosinophils, and a poor correlation for basophils in samples with low counts; for basophil counts of 2% or higher, we found an improvement of the correlation coefficient. In the lipid interference experiment, only hemoglobin determination was influenced significantly on the CD 4000, but by using a new Abbott hemoglobin reagent, the interference was eliminated. The CBC and differential counts were stable and reportable up to at least 24 hours. Intrasample viability information on leukocytes provided a quality check on each individual specimen.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Grimaldi E,Scopacasa Fdoi
10.1309/L7LC-6KKJ-12G2-196Wsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
497-505issue
4eissn
0002-9173issn
1943-7722journal_volume
113pub_type
杂志文章abstract::Immunohistochemical expression of napsin A in primary pulmonary mucinous tumors is not well established. Napsin A immunoreactivity was evaluated in 43 mucin-producing adenocarcinomas of the lung consisting of 18 tumors formerly classified as mucinous bronchioloalveolar carcinoma, 15 colloid adenocarcinomas, 5 solid pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP62WJUAMSZCOM
更新日期:2013-02-01 00:00:00
abstract::The authors tested a newly-developed computerized laser scanning cytometer (LSC) as a means of performing immunophenotypic analysis of hematologic specimens within their community hospital. Results were compared on a case-by-case basis with parallel flow cytometric and immunohistochemical data. A total of 71 specimens...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/105.6.744
更新日期:1996-06-01 00:00:00
abstract::Gastric intraepithelial neoplasia (IEN; formerly dysplasia) is an advanced precancerous lesion. Lesions indefinite for IEN mimic the IEN phenotype but lack some morphologic attributes of IEN. Indefinite for IEN lesions may arise in native foveolae (atypical foveolar hyperproliferation [aFH]) or intestinalized glands (...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPEU41HTGXSJDQ
更新日期:2012-05-01 00:00:00
abstract::Three paired samples were distributed at three--six-month intervals in the Basic Ligand Survey during 1976. The stabilities of mean values obtained by widely used commercial kit procedures and by all methods for eight analytes are reported. Significant improvements in accuracy and long-term precision were identified i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.4.537
更新日期:1975-10-01 00:00:00
abstract::Estrogen and progesterone receptors were studied in fine-needle aspiration biopsy specimens of 56 patients with primary, recurrent, or metastatic breast carcinoma. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesterone hemisuccinate...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/95.1.35
更新日期:1991-01-01 00:00:00
abstract::We describe 35 peripheral lymph nodes classified as mantle cell/marginal zone B-cell hyperplasia with clear cells using morphologic and immunologic findings. For the purpose of this study, we obtained clinical follow-up information and performed immunoglobulin gene rearrangement studies on paraffin sections by polymer...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/P2M2-JEA3-YYQF-0P38
更新日期:2001-10-01 00:00:00
abstract::Serologic testing shows that hepatitis C virus (HCV) may have a role in the pathogenesis of B-cell non-Hodgkin lymphomas (B-cell NHLs). We tried to demonstrate HCV RNA sequences in paraffin-embedded tissue from B-cell NHLs by reverse-transcription double polymerase chain reaction (RT-PCR) and Southern blotting. We stu...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/REV9-FDTM-5NGC-HBWY
更新日期:2000-03-01 00:00:00
abstract::Patient red blood cell (RBC) index means, when used in quality control, form an independent standard that is as accurate and precise as preserved blood controls. If such patient data are routinely incorporated in intralaboratory and interlaboratory quality control programs, a substantial improvement in the present sta...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.2.193
更新日期:1986-08-01 00:00:00
abstract::Therapeutic control achieved by an anticoagulant clinic may be assessed by calculation of the proportion of time spent by the patients within the prothrombin time target range aimed for by the clinic. Three calculation methods for such assessment have been described by other investigators. The three methods were compa...
journal_title:American journal of clinical pathology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1093/ajcp/90.6.685
更新日期:1988-12-01 00:00:00
abstract::Transketolases link the Embden-Meyerhof pathway to the pentose phosphate pathway. An influence of p-Akt on this metabolism was described. This study was performed to compare the expression of transketolase-like 1 (TKTL1) and p-Akt in glioblastoma multiforme (GBM) and other astrocytic gliomas (AGs, grades II and III). ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/6H9844AMMET82DBJ
更新日期:2008-07-01 00:00:00
abstract::Increasingly, all automated blood counts are not accompanied by a microscopic white blood cell differential. A popular strategy is to obtain a manual differential if any part of the automated blood count and differential is outside specified limits (the "diff-if" strategy). The authors compared two sets of criteria to...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/90.2.163
更新日期:1988-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the expression of progesterone receptor (PR), estrogen receptor (ER), and G protein-coupled estrogen receptor 1 (GPER-1) in cutaneous neurofibromas (cNFs) and their correlation with demographic, clinical, and laboratory data of individuals with neurofibromatosis 1 (NF1). The association of PROGIN...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa186
更新日期:2020-12-08 00:00:00
abstract::Specific esterase activity, a property generally ascribed to cells of granulocytic origin, was found in abnormal erythroid precursors obtained from the bone marrows of six patients with untreated erythroleukemia. These erythroblasts also showed nonspecific esterase activity. Neither specific nor nonspecific esterase a...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/67.4.368
更新日期:1977-04-01 00:00:00
abstract::Twenty cases of inflammatory myofibroblastic tumor (IMT) were studied; 19 involved the lung and 1 the esophagus only. The patients' ages ranged from 3 to 72 years. There were 9 males and 11 females. Involvement of a bronchus was seen in one case and of mediastinal structures in four. Chest pain and dyspnea were common...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.5.538
更新日期:1990-11-01 00:00:00
abstract::Studies from Africa have demonstrated that black people have higher vitamin B12 (cobalamin) levels than do white people. The authors compared healthy white people, black people, and Latin-Americans in the United States. Their study is also the largest in which the effect of race and sex on the cobalamin levels has bee...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.1.95
更新日期:1987-07-01 00:00:00
abstract::Our purpose was to determine whether the in vitro (or "spiked curve") method for the therapeutic heparin range is an accurate and valid method for heparin monitoring. Many laboratories use the in vitro method for determining the activated partial thromboplastin time (APTT)-based unfractionated heparin (UFH) therapeuti...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP60ZGXCJKRMJO
更新日期:2011-06-01 00:00:00
abstract::The participant performances on the College of American Pathologists (CAP) Urine Survey specimens distributed during 1979 and 1980 for pregnancy testing are reviewed. Almost all the reagent methods used by the participants performed well on the negative specimens. Prominent differences in results on the positive speci...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::Nine human exocrine pancreatic adenocarcinomas were examined by serial sectioning and double- and triple-labeled immunohistochemical techniques with antibodies against chromogranin A, insulin, islet amyloid polypeptide, glucagon, somatostatin, pancreatic polypeptide, serotonin, pancreastatin, and neuron-specific enola...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/100.3.223
更新日期:1993-09-01 00:00:00
abstract::Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. It has become apparent that rFVIIa is useful in controlling bleeding in a variety of clinical situations. This review attempts to collate and summarize the nonhemophilia...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/D0G0-C96V-05CJ-5B4J
更新日期:2004-01-01 00:00:00
abstract::Utilizing a bacterial-agar overlay technic incorporating the methionine-requiring bacterium Leukonostoc mesenteroides, little or no bacterial growth was seen surrounding the megaloblasts and proerythroblasts of eight patients who had severe untreated pernicious anemia. Similarly, scant bacterial growth was observed in...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/65.4.504
更新日期:1976-04-01 00:00:00
abstract::The availability of automated anti-Xa heparin assays provides the opportunity to manage patient unfractionated heparin levels directly, rather than by the activated partial thromboplastin time. Because critically ill patients can acquire an antithrombin deficiency, we compared the performance of 3 anti-Xa heparin assa...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/8E3U7RXEPXNP27R7
更新日期:2006-09-01 00:00:00
abstract::The immunohistochemical staining patterns of three pulmonary blastomas (PBs) were studied for markers of mesenchymal (vimentin, muscle-specific actin) and epithelial (cytokeratin, epithelial membrane antigen) differentiation to clarify their phenotypic nature and, possibly, their histogenesis. Epithelial markers were ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.6.773
更新日期:1988-06-01 00:00:00
abstract::Human intravenous immunoglobulins prepared by the cold ethanol fractionation technique of Cohn are considered safe with respect to infectivity. However, there have been several instances of transmission of both hepatitis B and non-A,non-B hepatitis viruses after administration of intravenous immunoglobulins. To determ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/97.1.108
更新日期:1992-01-01 00:00:00
abstract:OBJECTIVES:Human epidermal growth factor receptor 2 (HER2, ERBB2) testing is an important prognostic/predictive marker in breast cancer management, especially in selecting HER2-targeted treatment. American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines address HER2 status and were...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP3Q9WFOQTKUVV
更新日期:2015-08-01 00:00:00
abstract:Objectives:According to current guidelines, either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (Her2/neu) status in breast carcinoma. While the guidelines explicitly delineate result interpretation, there is no consensus on the most appropr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw224
更新日期:2017-04-01 00:00:00
abstract::Progression of follicular lymphoma (FL) to a higher-grade lymphoma occurs in 25% to 60% of cases and frequently indicates a poor prognosis. The transformation is accompanied by alteration in morphologic features and clinical behaviors. Herein, we report a case of FL occurring in a 59-year-old woman with the developmen...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/NKK3FEX2BE5L7EKB
更新日期:2008-01-01 00:00:00
abstract::To study the histogenesis of and determine the most useful markers for diagnosing anaplastic thyroid carcinoma (ATC), 32 cases, including 2 with numerous osteoclast-like cells, were stained with a battery of antibodies to epithelial (keratin, epithelial membrane antigen [EMA], carcinoembryonic antigen [CEA]), mesenchy...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/96.1.15
更新日期:1991-07-01 00:00:00
abstract::We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4QUN-J4F2-7QK7-RR0G
更新日期:2004-11-01 00:00:00
abstract:Objectives:The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings. Methods:Pooled fresh plasma is aliquoted into 2-mL Eppen...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqy031
更新日期:2018-07-03 00:00:00