Comparison of Affirm VPIII and Papanicolaou tests in the detection of infectious vaginitis.

Abstract:

:To compare the Affirm VPIII molecular test (Becton Dickinson, Burlington, NC) with morphologic identification used in routine Papanicolaou (Pap) test screening in the detection and identification of Candida species, Trichomonas vaginalis, and Gardnerella vaginalis, we identified 431 cases with a concomitant Pap test and Affirm VPIII assay performed from the archives of a large academic institution. The study population consisted of women ranging in age from 17 to 79 years (mean and median ages, 33 and 31 years, respectively). With a routine Pap test, 60 patients (13.9%) were found to have bacterial vaginosis, 60 (13.9%) candidiasis, and 3 (0.7%) Trichomonas infection. With the Affirm VPIII assay, 183 (42.5%) patients tested positive for G vaginalis, 70 (16.2%) positive for Candida species, and 10 (2.3%) positive for T vaginalis. The differences were statistically significant. The results demonstrate that our patient population had a high incidence of bacterial vaginosis/Candida vaginitis; however, the Affirm VPIII was a more sensitive diagnostic test for the detection and identification of all 3 organisms compared with the Pap test.

journal_name

Am J Clin Pathol

authors

Levi AW,Harigopal M,Hui P,Schofield K,Chhieng DC

doi

10.1309/AJCP7TBN5VZUGLZU

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

442-7

issue

3

eissn

0002-9173

issn

1943-7722

pii

135/3/442

journal_volume

135

pub_type

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