Comparison of Affirm VPIII and Papanicolaou tests in the detection of infectious vaginitis.


:To compare the Affirm VPIII molecular test (Becton Dickinson, Burlington, NC) with morphologic identification used in routine Papanicolaou (Pap) test screening in the detection and identification of Candida species, Trichomonas vaginalis, and Gardnerella vaginalis, we identified 431 cases with a concomitant Pap test and Affirm VPIII assay performed from the archives of a large academic institution. The study population consisted of women ranging in age from 17 to 79 years (mean and median ages, 33 and 31 years, respectively). With a routine Pap test, 60 patients (13.9%) were found to have bacterial vaginosis, 60 (13.9%) candidiasis, and 3 (0.7%) Trichomonas infection. With the Affirm VPIII assay, 183 (42.5%) patients tested positive for G vaginalis, 70 (16.2%) positive for Candida species, and 10 (2.3%) positive for T vaginalis. The differences were statistically significant. The results demonstrate that our patient population had a high incidence of bacterial vaginosis/Candida vaginitis; however, the Affirm VPIII was a more sensitive diagnostic test for the detection and identification of all 3 organisms compared with the Pap test.


Am J Clin Pathol


Levi AW,Harigopal M,Hui P,Schofield K,Chhieng DC




Has Abstract


2011-03-01 00:00:00














  • Recombinant protein to analyze autoantibodies to proteinase 3 in systemic vasculitis.

    abstract::The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Rarok AA,Huitema MG,van der Leij MJ,van der Geld YM,Berthold H,Schmitt J,Stegeman CA,Limburg PC,Kallenberg CG

    更新日期:2003-10-01 00:00:00

  • pH, PCO2 and PO2 in "high-volume" platelet concentrates prepared by discontinuous-flow centrifugation and stored in polyvinylchloride and plyethylene containers.

    abstract::High-volume (200 ml) platelet concentrates prepared by discontinuous-flow centrifugation, with counts as high as ca. 2.0 x 10(12)/l, were stored in either 600 ml-capacity polyvinylchloride bags or 800-ml capacity polyethylene bags. Both polyvinylchloride and polyethylene bags appeared adequate for 24-hour storage of p...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Rosenstein R,Lawe JE,North JG

    更新日期:1980-03-01 00:00:00

  • The New Equivocal: Changes to HER2 FISH Results When Applying the 2013 ASCO/CAP Guidelines.

    abstract:OBJECTIVES:Human epidermal growth factor receptor 2 (HER2, ERBB2) testing is an important prognostic/predictive marker in breast cancer management, especially in selecting HER2-targeted treatment. American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines address HER2 status and were...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Long TH,Lawce H,Durum C,Moore SR,Olson SB,Gatter K,Troxell ML

    更新日期:2015-08-01 00:00:00

  • The effects of under 6 hours of formalin fixation on hormone receptor and HER2 expression in invasive breast cancer: a systematic review.

    abstract:OBJECTIVES:A systematic review of the literature was performed to identify whether minimum formalin fixation time may be reduced for reliable immunohistochemical assessment of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). METHODS:PubMed, EMBASE, and the Cochra...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审


    authors: Kalkman S,Barentsz MW,van Diest PJ

    更新日期:2014-07-01 00:00:00

  • Acute myelofibrosis. Immunohistochemical study of four cases and comparison with acute megakaryocytic leukemia.

    abstract::Four cases of acute myelofibrosis are contrasted to three cases of acute megakaryocytic leukemia. The cases were clinically indistinguishable. Light microscopic examination of hematoxylin and eosin-stained bone marrow biopsy specimens from the cases of acute myelofibrosis showed a trilinear proliferation of mature and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Hruban RH,Kuhajda FP,Mann RB

    更新日期:1987-11-01 00:00:00

  • ABH blood group isoantigen expression in breast carcinomas--an immunohistochemical evaluation using monoclonal antibodies.

    abstract::The ABH blood group isoantigen status of a retrospective series of 233 invasive breast carcinomas was examined, employing monoclonal antibodies (MCAB) against A, B, and H antigens with the avidin-biotin-peroxidase complex method. In addition, the H antigen was localized with Ulex Europeus Agglutinin I (UEAI) binding. ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Lee AK,DeLellis RA,Rosen PP,Saigo PE,Gangi MD,Bagin R,Groshen S,Wolfe HJ

    更新日期:1985-03-01 00:00:00

  • Misleading hepatitis C serology following administration of intravenous immunoglobulin.

    abstract::Intravenous immunoglobulin (IVIG) is a blood derivative that is being evaluated for the prevention and treatment of numerous disorders. Recent studies have suggested beneficial effects from the prophylactic administration of IVIG to very-low-birth-weight neonates. A disadvantage of such therapy is misleading infectiou...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Nixon RR,Smith SA,Johnson RL,Pillers DA

    更新日期:1994-03-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Phenotypic heterogeneity of B cells in patients with chronic lymphocytic leukemia/small lymphocytic lymphoma.

    abstract::Although some studies have examined the expression of aberrant markers such as CD2, CD7, CD10, CD13, CD33, and CD34 on B cells in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), a uniform multiparametric analysis of the frequency of expression of these markers using stringent criteria is lacking. By...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Kampalath B,Barcos MP,Stewart C

    更新日期:2003-06-01 00:00:00

  • S-100 protein positive human T-lymphocyte.

    abstract::S-100 protein was detected in a small number of human peripheral T-lymphocytes by a direct immunoperoxidase method with the use of monospecific antibody to S-100 protein. Complemented-mediated lysis using monoclonal antibodies revealed that the S-100+ T-lymphocytes bore OKT3, OKT8, and OKT11 antigens but not OKT4, OKM...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Takahashi K,Isobe T,Ohtsuki Y,Sonobe H,Yamaguchi H,Akagi T

    更新日期:1985-01-01 00:00:00

  • Comparison of disagreement and amendment rates by tissue type and diagnosis: identifying cases for directed blinded review.

    abstract::We sought to determine whether a group of cases that was relatively high in disagreements and subsequent amendments could be identified and targeted for blinded review. During a 4-year period, 8,916 surgical pathology and nongynecologic cytology cases were subjected to blinded review; of these, there were 616 disagree...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Renshaw AA,Gould EW

    更新日期:2006-11-01 00:00:00

  • Real-Time Clinical Decision Support Decreases Inappropriate Plasma Transfusion.

    abstract:Objectives:To curtail inappropriate plasma transfusions, we instituted clinical decision support as an alert upon order entry if the patient's recent international normalized ratio (INR) was 1.7 or less. Methods:The alert was suppressed for massive transfusion and within operative or apheresis settings. The plasma ord...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Shah N,Baker SA,Spain D,Shieh L,Shepard J,Hadhazy E,Maggio P,Goodnough LT

    更新日期:2017-08-01 00:00:00

  • Comparative study of spleen pathology in drug abusers with thrombocytopenia related to human immunodeficiency virus infection and in patients with idiopathic thrombocytopenic purpura. A morphometric, immunohistochemical, and ultrastructural study.

    abstract::A morphometric and immunohistochemical study was performed to assess the spleen's alterations in patients with autoimmune thrombocytopenia and in drug abusers with thrombocytopenia (DAT) related to human immunodeficiency virus (HIV) infection. A total of 34 patients were included in the study: 20 DAT patients and 14 w...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Marti M,Feliu E,Campo E,Palacin A,Berga L,Casals FJ,Urbano Ispizua A,Cardesa A,Rozman C

    更新日期:1993-12-01 00:00:00

  • Double immunostaining of lymph node sections by monoclonal antibodies using phycoerythrin labeling and haptenated reagents.

    abstract::Double immunostaining of tissue sections by monoclonal antibodies has been performed using, in immunofluorescence, combinations of reagents conjugated with different fluorochromes. These were monoclonal antibodies directly labeled with phycoerythrin (a new red fluorochrome) and haptenated monoclonal antibodies detecte...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Pizzolo G,Chilosi M

    更新日期:1984-07-01 00:00:00

  • Recombinant factor VIIa in the treatment of bleeding.

    abstract::Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. It has become apparent that rFVIIa is useful in controlling bleeding in a variety of clinical situations. This review attempts to collate and summarize the nonhemophilia...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审


    authors: Midathada MV,Mehta P,Waner M,Fink LM

    更新日期:2004-01-01 00:00:00

  • Primary carcinoid of the common bile duct. Immunohistochemical characterization of a case and review of the literature.

    abstract::A 39-year-old woman with a primary carcinoid of the common bile duct is presented. The tumor showed argyrophilia; and, by immunohistochemical studies, chromogranin, serotonin, and somatostatin were demonstrated. The patient has no symptoms 42 months after surgical treatment. To the authors' knowledge, this is the firs...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审


    authors: Angeles-Angeles A,Quintanilla-Martínez L,Larriva-Sahd J

    更新日期:1991-09-01 00:00:00

  • An improved method for evaluation of blood coagulation in heparinized blood.

    abstract::A simple method designed to allow coagulation studies in heparinized blood was evaluated. Citrated plasma containing heparin was incubated with an anion-exchange resin commercially available in tablet form (Heparsorb). After centrifugation of the mixture, coagulation studies were carried out on the supernatant plasma....

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Cowan JF,Khan MB,Vargo J,Joist JH

    更新日期:1981-01-01 00:00:00

  • Indirect methods of assessing thyroid function. Protein-bound iodine and thyroxine by column chromatography, a 1976 CAP survey.

    abstract::An assessment of the infrequently performed protein-bound iodine determinations (PBI) and thyroxine (T4) determinations by column chromatography is presented after analysis of data contributed by participants in the 1976 College of American Pathologists Chemistry Survey Programs. The two technics yield comparable mean...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Thiessen MM,Batsakis JG,Neuhoff J,Thomas BP

    更新日期:1978-09-01 00:00:00

  • Endometrial stromal sarcomas: immunoprofile with emphasis on HMB45 reactivity.

    abstract:OBJECTIVES:We describe the morphologic and immunohistochemical features of 17 endometrial stromal neoplasms, 16 sarcomas, and one stromal nodule. METHODS:We reviewed 35 cases interpreted as endometrial stromal neoplasms, but 17 high-grade endometrial stromal sarcomas (ESS) and one case of mixed endometrial sarcoma and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Albores-Saavedra J,Dorantes-Heredia R,Chablé-Montero F,Chanona-Vilchis J,Pérez-Montiel D,Lino-Silva LS,González-Romo MA,Ramírez-Jaramillo JM,Henson DE

    更新日期:2014-06-01 00:00:00

  • Spurious red blood cell parameters due to serum cold agglutinins: observations on Ortho ELT-8 cell counter.

    abstract::The pattern of spurious red blood cell parameters caused by serum cold agglutinins has been well characterized for the Coulter counters but not the Ortho ELT-8. The pattern consists of a spurious increase in mean corpuscular volume (MCV), a discrepancy between the hemoglobin and the hematocrit, the latter falsely redu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Solanki DL,Blackburn BC

    更新日期:1985-02-01 00:00:00

  • Pathologic findings from the national surgical adjuvant breast project (protocol no. 4). II. The significance of regional node histology other than sinus histiocytosis in invasive mammary cancer.

    abstract::The morphologic appearances of regional lymph nodes in radical mastectomy specimens obtained from 303 women entered into a prospective study of invasive breast cancer were categorized into patterns that have been considered to reflect immunologic function. An attempt was made to correlate these with 31 other histologi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Fisher ER,Gregorio R,Redmond C,Dekker A,Fisher B

    更新日期:1976-01-01 00:00:00

  • Paraffin-resistant antigens detectable by antibodies L26 and polyclonal CD3 predict the B- or T-cell lineage of 95% of diffuse aggressive non-Hodgkin's lymphomas.

    abstract::The reactivity of eight preferential B-cell (L26, 4KB5, and KiB3) and T-cell (polyclonal CD3, Leu22, MT-1, UCHL-1, and OPD4) antibodies which detect paraffin-resistant antigens was examined by a three-step immunoperoxidase technique in 111 formalin-fixed, paraffin-embedded diffuse aggressive non-Hodgkin's lymphomas (N...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Chadburn A,Knowles DM

    更新日期:1994-09-01 00:00:00

  • Babesiosis: problems in diagnosis using autoanalyzers.

    abstract::A 76-year-old white man previous diagnosed as having Waldenstrom's macroglobulinemia continued with persistent fevers and sweats for two and a half years. Recently, repeated automated differentials during 11 days of hospitalization failed to note any intracellular inclusions in the RBCs. Blood sent to the Microbiology...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Bruckner DA,Garcia LS,Shimizu RY,Goldstein EJ,Murray PM,Lazar GS

    更新日期:1985-04-01 00:00:00

  • Immunohistochemical detection of XIAP in mesothelium and mesothelial lesions.

    abstract::We examined benign and malignant mesothelial tissue samples for the presence of X-linked inhibitor of apoptosis protein (XIAP), a potent constituent of the inhibitor of apoptosis family of caspase inhibitors. We subjected 55 sections (31 malignant mesotheliomas, 2 well-differentiated peritoneal mesotheliomas, 13 pleur...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Wu M,Sun Y,Li G,Desman G,Wang B,Gil J,Burstein DE

    更新日期:2007-11-01 00:00:00

  • MACC1 is related to colorectal cancer initiation and early-stage invasive growth.

    abstract:OBJECTIVES:To investigate metastasis associated in colon cancer 1 (MACC1) and MET expression in colorectal adenoma, Tis, early-stage invasive (T1 and T2), and advanced adenocarcinoma with liver metastasis using immunohistochemistry. METHODS:Ninety-three paraffin-embedded colorectal tumor specimens were immunohistochem...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Ren B,Zakharov V,Yang Q,McMahon L,Yu J,Cao W

    更新日期:2013-11-01 00:00:00

  • Immunophenotypic profile of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia.

    abstract::We retrospectively reviewed the immunophenotypic profile of 75 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) analyzed by flow cytometry. All patients had monoclonal IgM (median, 2,100 mg/dL [21 g/L]) in serum and were considered clinically to have WM. The neoplastic cells, in all cases, ex...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Konoplev S,Medeiros LJ,Bueso-Ramos CE,Jorgensen JL,Lin P

    更新日期:2005-09-01 00:00:00

  • Impact of Laboratory Charge Display Within the Electronic Health Record Across an Entire Academic Medical Center: Results of a Randomized Controlled Trial.

    abstract:Objectives:To determine the impact of systemwide charge display on laboratory utilization. Methods:This was a randomized controlled trial with a baseline period and an intervention period. Tests were randomized to a control arm or an active arm. The maximum allowable Medicare reimbursement rate was displayed for tests...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,随机对照试验


    authors: Schmidt RL,Colbert-Getz JM,Milne CK,Vargo DJ,Hussong JW,Hoidal JR,Markewitz BA,Walker BS,Kawamoto K

    更新日期:2017-11-20 00:00:00

  • Comparison of the E-test and reference agar dilution method for susceptibility of gram-negative anaerobic organisms.

    abstract::Minimal inhibitory concentrations (MICs) of clindamycin, cefoxitin, imipenem, and metronidazole were determined by the E-Test and a reference agar dilution method for 92 gram-negative anaerobic organisms. For 335 MIC pairs, the agreement between the two systems was 80.1%; 60 (17.9%) differed by two or more twofold dil...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Couroux PR,Massey VE,Schieven BC,Lannigan R,Hussain Z

    更新日期:1993-09-01 00:00:00

  • Bladder Cancer Involving Smooth Muscle of Indeterminate Type or Muscularis Mucosae in Transurethral Biopsy Specimens.

    abstract:OBJECTIVES:Bladder cancers invading the muscularis mucosae (MM) are treated differently from those invading the muscularis propria (MP). However, it may be difficult to determine the type of smooth muscle in transurethral resection (TUR) or biopsy specimens. We aimed to investigate the clinicopathologic features of bla...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Hwang MJ,Kamat AM,Dinney CP,Czerniak B,Guo CC

    更新日期:2020-07-07 00:00:00

  • Differentiation of primary and secondary cutaneous B-cell lymphoma by Southern blot analysis.

    abstract::Malignant B-cell lymphomas represent a heterogenous group of lymphoreticular disorders that involve the skin in about 20% of reported cases. Skin involvement may be primary or secondary (ie, the result of hematogenous spread). Primary cutaneous B-cell lymphomas (PCBCLs) are thought to take a comparatively favorable co...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章


    authors: Sepp N,Weyer K,Haun M,Zelger B,Thaler J,Faber V,Fritsch P,Grünewald K

    更新日期:1996-12-01 00:00:00