Abstract:
:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from A. tumefaciens infiltrated leaves were obtained with an efficiency of about 34%. In 20% of the regenerants bar gene excision was due to the expression of stably integrated cre gene, whereas in 14% of plants site-specific recombination was a consequence of transient cre expression. Phenotypic and molecular data indicated that the recombined state has been transferred to the T(1 )generation. These results demonstrate the suitability of agroinfiltration for the expression of cre recombinase in vivo.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Kopertekh L,Schiemann Jdoi
10.1007/s11248-005-8293-7subject
Has Abstractpub_date
2005-10-01 00:00:00pages
793-8issue
5eissn
0962-8819issn
1573-9368journal_volume
14pub_type
杂志文章abstract::The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1018423015014
更新日期:1997-09-01 00:00:00
abstract::Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9759-7
更新日期:2014-04-01 00:00:00
abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9350-4
更新日期:2010-08-01 00:00:00
abstract::We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008972010351
更新日期:2000-06-01 00:00:00
abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9556-0
更新日期:2012-06-01 00:00:00
abstract::We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9146-3
更新日期:2008-08-01 00:00:00
abstract::A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaer...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01969382
更新日期:1993-03-01 00:00:00
abstract::The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1015210919077
更新日期:2002-04-01 00:00:00
abstract::With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9181-8
更新日期:2008-12-01 00:00:00
abstract::Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9715-6
更新日期:2013-12-01 00:00:00
abstract::Sugarbeets carrying superoxide dismutase transgenes were developed in order to investigate the possibility of enhancing their resistance to oxidative stress. Binary T-DNA vectors carrying the chloroplastic and cytosolic superoxide dismutase genes from tomato, were used for Agrobacterium-mediated transformation of suga...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000034610.35724.04
更新日期:2004-06-01 00:00:00
abstract::Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9107-x
更新日期:2007-12-01 00:00:00
abstract::We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-9587-x
更新日期:2004-10-01 00:00:00
abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1771-0
更新日期:2005-12-01 00:00:00
abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00208-9
更新日期:2020-08-01 00:00:00
abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976765
更新日期:1994-11-01 00:00:00
abstract::The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9495-9
更新日期:2011-12-01 00:00:00
abstract::The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-014-9826-8
更新日期:2014-10-01 00:00:00
abstract::The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008915007271
更新日期:1999-06-01 00:00:00
abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1024267416522
更新日期:2003-08-01 00:00:00
abstract::Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9388-3
更新日期:2011-02-01 00:00:00
abstract::Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008951732020
更新日期:2001-04-01 00:00:00
abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4350-5
更新日期:2005-10-01 00:00:00
abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008966404981
更新日期:2000-01-01 00:00:00
abstract::To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9328-2
更新日期:2010-06-01 00:00:00
abstract::Transgenic mice carrying the 3-hydroxy-3-methylglutarylCoA reductase (HMG) promoter driving the Escherichia coli beta-galactosidase (lacZ) gene did not display the expected ubiquitous and constitutive expression in HMG-lacZ transgenic mice. The same promoter is however able to drive ubiquitous expression of the chlora...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976173
更新日期:1993-11-01 00:00:00
abstract::The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5788-6
更新日期:2005-02-01 00:00:00
abstract::Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5413-8
更新日期:2005-04-01 00:00:00
abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
abstract::In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0039-9
更新日期:2017-12-01 00:00:00