Abstract:
:Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments. There is emerging evidence that peripheral blood T lymphocytes from patients with dermatitis have significantly increased telomerase activity. Here, we developed telomerase reverse transcriptase (TERT)-expressing transgenic (Tg) rats that spontaneously developed eczematous skin inflammation in childhood. Newborn TERT-Tg rats developed visible dermatitis in 56 % of cases, and the skin lesions microscopically showed spongiosis and acanthosis with infiltration of lymphocytes, eosinophils and mast cells. TERT-Tg rats with dermatitis exhibited increased CD4 (2.5-fold) and CD8 (fivefold) T cell numbers compared with dermatitis-free TERT-Tg rats. Stronger TERT activity was observed in the peripheral lymphocytes of dermatitis-positive TERT-Tg rats than those of dermatitis-free TERT-Tg rats. RT-PCR analysis revealed that IL-4 was markedly elevated in the spleen of dermatitis-positive TERT-Tg rats, and that interferon-gamma was increased in the dermatitis lesions. Moreover, skin grafting of TERT-Tg rats with dermatitis onto T cell-deficient nude rats demonstrated that the inflamed skin lesions could not be maintained. Taken together, the results suggest that TERT activation in T lymphocytes is one of the potential predisposing factors for dermatitis. Moreover, our results demonstrated that the TERT-Tg rats mirror aspects of human childhood-onset dermatitis and that these animals represent a potential animal model system for studying childhood-onset dermatitis.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami Tdoi
10.1007/s11248-016-9939-3subject
Has Abstractpub_date
2016-08-01 00:00:00pages
413-24issue
4eissn
0962-8819issn
1573-9368pii
10.1007/s11248-016-9939-3journal_volume
25pub_type
杂志文章abstract::Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9800-5
更新日期:2014-08-01 00:00:00
abstract::Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000005146.05655.7d
更新日期:2003-12-01 00:00:00
abstract::To characterize the activity of the polyomavirus regulatory region, two hybrid marker genes were constructed. In the first construct, the early promoter regulates expression of the CAT gene and the late promoter regulates expression of the lacZ gene. In the second construct, the lacZ gene was placed under the control ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01980202
更新日期:1996-11-01 00:00:00
abstract::We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalen...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008972010351
更新日期:2000-06-01 00:00:00
abstract::Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00
abstract::Gonadal effects of the Denys-Drash syndrome (DDS) mutation Wt1(tmT396 )were examined in chimaeric and heterozygous mice. Since the only heterozygote was 41,XXY, Sertoli cell function was assessed by comparison with age-matched control XXY testes. Control XXY Sertoli cells showed immuno-expression of WT1 and androgen r...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-7216-y
更新日期:2005-10-01 00:00:00
abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02512996
更新日期:1991-12-01 00:00:00
abstract::This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9858-0
更新日期:2015-06-01 00:00:00
abstract::The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-015-9899-z
更新日期:2015-10-01 00:00:00
abstract::Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9817-9
更新日期:2015-02-01 00:00:00
abstract::Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9479-1
更新日期:2011-10-01 00:00:00
abstract::The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00175-w
更新日期:2019-12-01 00:00:00
abstract::As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9122-y
更新日期:2007-12-01 00:00:00
abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9969-x
更新日期:2016-12-01 00:00:00
abstract::To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title:Transgenic research
pub_type: 信件
doi:10.1007/s11248-012-9605-3
更新日期:2012-12-01 00:00:00
abstract::We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1773-y
更新日期:2005-12-01 00:00:00
abstract::The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9629-8
更新日期:2013-02-01 00:00:00
abstract::A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaer...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01969382
更新日期:1993-03-01 00:00:00
abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00110-z
更新日期:2019-04-01 00:00:00
abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9180-9
更新日期:2008-10-01 00:00:00
abstract::SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in ou...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00215-w
更新日期:2020-12-01 00:00:00
abstract::Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/BF01976021
更新日期:1994-01-01 00:00:00
abstract::The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9495-9
更新日期:2011-12-01 00:00:00
abstract::Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9585-8
更新日期:2012-10-01 00:00:00
abstract::Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
abstract::Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5413-8
更新日期:2005-04-01 00:00:00
abstract::RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9765-9
更新日期:2014-04-01 00:00:00
abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4350-5
更新日期:2005-10-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9106-y
更新日期:2008-06-01 00:00:00