Abstract:
:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV-ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady-state RNA analysis and nuclear run-on assay of a line showed that the resistance was RNA-mediated through the post-transcriptional gene silencing mechanism.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Jan FJ,Pang SZ,Fagoaga C,Gonsalves Ddoi
10.1023/a:1008915007271subject
Has Abstractpub_date
1999-06-01 00:00:00pages
203-13issue
3eissn
0962-8819issn
1573-9368journal_volume
8pub_type
杂志文章abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016374128479
更新日期:2002-08-01 00:00:00
abstract::Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9735-2
更新日期:2014-02-01 00:00:00
abstract::A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016671513425
更新日期:2001-08-01 00:00:00
abstract::Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9300-1
更新日期:2010-04-01 00:00:00
abstract::It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9785-0
更新日期:2014-12-01 00:00:00
abstract::Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1008966404981
更新日期:2000-01-01 00:00:00
abstract::Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9825-9
更新日期:2015-02-01 00:00:00
abstract::Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
abstract::A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01976765
更新日期:1994-11-01 00:00:00
abstract::The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9477-3
更新日期:2011-10-01 00:00:00
abstract::To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title:Transgenic research
pub_type: 信件
doi:10.1007/s11248-012-9605-3
更新日期:2012-12-01 00:00:00
abstract::A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1013007329936
更新日期:2001-12-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-3913-9
更新日期:2006-04-01 00:00:00
abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9180-9
更新日期:2008-10-01 00:00:00
abstract::Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9709-4
更新日期:2013-10-01 00:00:00
abstract::Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9364-y
更新日期:2010-10-01 00:00:00
abstract::This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9688-5
更新日期:2013-10-01 00:00:00
abstract::We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9711-x
更新日期:2013-12-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9494-x
更新日期:2011-12-01 00:00:00
abstract::Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9821-0
更新日期:2015-02-01 00:00:00
abstract::Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0021-4
更新日期:2006-08-01 00:00:00
abstract::Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9817-9
更新日期:2015-02-01 00:00:00
abstract::T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1025814814823
更新日期:2003-10-01 00:00:00
abstract::The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9461-y
更新日期:2011-08-01 00:00:00
abstract::We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02513027
更新日期:1992-03-01 00:00:00
abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4350-5
更新日期:2005-10-01 00:00:00
abstract::To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-006-0024-1
更新日期:2006-08-01 00:00:00
abstract::Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-1771-0
更新日期:2005-12-01 00:00:00
abstract::The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008928121846
更新日期:2000-02-01 00:00:00