Abstract:
:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat plants overexpressing each of the two wheat FKBPs: wFKBP73 (which is expressed in young vegetative and reproductive tissues under normal growth conditions) and wFKBP77 (which is induced by heat stress). Transgenic lines overexpressing wFKBP77 at 25 degrees C showed major morphological abnormalities, specifically relating to height, leaf shape, spike morphology and sterility. In these plants, the levels of hsp90 mRNA were over two fold higher than in controls, indicating a common regulatory pathway shared between wFKBP77 and Hsp90. Transgenic lines overexpressing wFKBP73 showed normal vegetative morphology, but the grain weight and composition was altered, corresponding to changes in amylase activity during seed development.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Kurek I,Stöger E,Dulberger R,Christou P,Breiman Adoi
10.1023/a:1016374128479subject
Has Abstractpub_date
2002-08-01 00:00:00pages
373-9issue
4eissn
0962-8819issn
1573-9368journal_volume
11pub_type
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journal_title:Transgenic research
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