Comparison of male chimeric mice generated from microinjection of JM8.N4 embryonic stem cells into C57BL/6J and C57BL/6NTac blastocysts.

abstract：:Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...

journal_title：Transgenic research

authors： Jiwan D,Roalson EH,Main D,Dhingra A

更新日期：2013-12-01 00:00:00

abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

journal_title：Transgenic research

authors： Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

更新日期：2014-04-01 00:00:00

abstract：:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...

journal_title：Transgenic research

authors： Béjar J,Hong Y,Alvarez MC

更新日期：2002-06-01 00:00:00

abstract：:Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New str...

journal_title：Transgenic research

authors： Soler E,Le Saux A,Guinut F,Passet B,Cohen R,Merle C,Charpilienne A,Fourgeux C,Sorel V,Piriou A,Schwartz-Cornil I,Cohen J,Houdebine LM

更新日期：2005-12-01 00:00:00

abstract：:A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

journal_title：Transgenic research

authors： Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

更新日期：2016-10-01 00:00:00

abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

journal_title：Transgenic research

authors： Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

更新日期：1994-11-01 00:00:00

abstract：:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

journal_title：Transgenic research

authors： Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

更新日期：2002-08-01 00:00:00

abstract：:The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...

journal_title：Transgenic research

authors： Ellens KW,Levac D,Pearson C,Savoie A,Strand N,Louter J,Tibelius C

更新日期：2019-08-01 00:00:00

abstract：:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

journal_title：Transgenic research

authors： Sivparsad BJ,Gubba A

更新日期：2014-04-01 00:00:00

abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

journal_title：Transgenic research

authors： Hisatsune C,Ogawa N,Mikoshiba K

更新日期：2013-12-01 00:00:00

abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

journal_title：Transgenic research

authors： Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

更新日期：2015-06-01 00:00:00

abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...

journal_title：Transgenic research

authors： Khan SA,Zafar Y,Briddon RW,Malik KA,Mukhtar Z

更新日期：2006-06-01 00:00:00

abstract：:We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...

journal_title：Transgenic research

authors： Dorin JR,Dickinson P,Emslie E,Clarke AR,Dobbie L,Hooper ML,Halford S,Wainwright BJ,Porteous DJ

更新日期：1992-03-01 00:00:00

abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

journal_title：Transgenic research

authors： Ashikawa I,Mori M,Nakamura S,Abe F

更新日期：2014-08-01 00:00:00

abstract：:Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...

journal_title：Transgenic research

authors： Wang K,Li Z,Li Y,Zeng J,He C,Yang J,Liu D,Wu Z

更新日期：2013-10-01 00:00:00

abstract：:During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...

journal_title：Transgenic research

authors： Rindt H,Subramaniam A,Robbins J

更新日期：1995-11-01 00:00:00

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

journal_title：Transgenic research

authors： Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

更新日期：2016-08-01 00:00:00

abstract：:There has been considerable interest in pursuing phospholamban as a putative therapeutic target for overcoming depressed calcium handling in human heart failure. Studies predominantly done in mice have shown that phospholamban is a key regulator of sarcoplasmic reticulum calcium cycling and cardiac function. However, ...

journal_title：Transgenic research

authors： Pattison JS,Waggoner JR,James J,Martin L,Gulick J,Osinska H,Klevitsky R,Kranias EG,Robbins J

更新日期：2008-04-01 00:00:00

abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

journal_title：Transgenic research

authors： Zhang D,Corlet A,Fouilloux S

更新日期：2008-06-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...

journal_title：Transgenic research

authors： Hirabayashi M,Yoshizawa Y,Kato M,Tsuchiya T,Nagao S,Hochi S

更新日期：2009-02-01 00:00:00

abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...

journal_title：Transgenic research

authors： Liu J,Luo Y,Zheng L,Liu Q,Yang Z,Wang Y,Su J,Quan F,Zhang Y

更新日期：2013-10-01 00:00:00

abstract：:The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...

journal_title：Transgenic research

authors： Saini KS,Byrne CR,Leish Z,Pruss CA,Rigby NW,Brownlee AG,Nancarrow CD,Ward KA

更新日期：1996-11-01 00:00:00

abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

journal_title：Transgenic research

authors： O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

更新日期：2002-10-01 00:00:00

abstract：:The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gen...

journal_title：Transgenic research

authors： Fedorov LM,Haegel-Kronenberger H,Hirchenhain J

更新日期：1997-05-01 00:00:00

abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

journal_title：Transgenic research

authors： Pai CW,Chen YH

更新日期：2010-08-01 00:00:00

abstract：:At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...

journal_title：Transgenic research

authors： Dowd PF,Lagrimini LM

更新日期：2006-04-01 00:00:00

abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...

journal_title：Transgenic research

authors： Quijano CD,Brunner S,Keller B,Gruissem W,Sautter C

更新日期：2015-02-01 00:00:00

abstract：:We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...

journal_title：Transgenic research

authors： Mankin SL,Allen GC,Phelan T,Spiker S,Thompson WF

更新日期：2003-02-01 00:00:00