Abstract：
:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though the ES cell approach has up to now been limited to mice, there is an increasing interest to develop this technology in both model and commercial fish species, with so far promising results in the medaka and zebrafish. In this study, we present evidence regarding a long-term stable cell line (SaBE-1c), derived from embryonic cells of the aquaculture marine fish Sparus aurata which has been characterized for (i) cell proliferation, (ii) chromosome complement, (iii) molecular markers, and (iv) in vitro tests of pluripotency by alkaline phosphatase (AP) staining, telomerase activity, and induced cell differentiation. These cells have proved their pluripotent capacities by in vitro tests. Furthermore, we have demonstrated their ability to produce chimaeras and to contribute to the formation of tissues from all three embryonic germ layers. These features suggest that SaBE-1c cells have the potential for multiple applications for the ES technology in fish, with the added value of originating from an economically important species.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Béjar J,Hong Y,Alvarez MCdoi
10.1023/a:1015678416921subject
Has Abstractpub_date
2002-06-01 00:00:00pages
279-89issue
3eissn
0962-8819issn
1573-9368journal_volume
11pub_type
杂志文章abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-1773-y
更新日期：2005-12-01 00:00:00
abstract：:The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00175-w
更新日期：2019-12-01 00:00:00
abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9928-6
更新日期：2016-06-01 00:00:00
abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9441-2
更新日期：2011-06-01 00:00:00
abstract：:Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) c...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0087-9
更新日期：2018-10-01 00:00:00
abstract：:Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vasc...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9224-1
更新日期：2009-04-01 00:00:00
abstract：:Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0012-7
更新日期：2017-06-01 00:00:00
abstract：:The introduction of cloned and manipulated genetic material into the germline of an experimental organism is one of the most powerful tools of modern biology. In the case of the fruit fly, Drosophila melanogaster, there is also an unparalleled range of sophisticated genetic tools to facilitate subsequent analysis. In ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/BF01968780
更新日期：1995-05-01 00:00:00
abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9495-9
更新日期：2011-12-01 00:00:00
abstract：:A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016671513425
更新日期：2001-08-01 00:00:00
abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01976765
更新日期：1994-11-01 00:00:00
abstract：:Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9180-9
更新日期：2008-10-01 00:00:00
abstract：:One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9932-x
更新日期：2016-06-01 00:00:00
abstract：:We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022194120518
更新日期：2003-02-01 00:00:00
abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9688-5
更新日期：2013-10-01 00:00:00
abstract：:Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9107-x
更新日期：2007-12-01 00:00:00
abstract：:Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9709-4
更新日期：2013-10-01 00:00:00
abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9477-3
更新日期：2011-10-01 00:00:00
abstract：:Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9479-1
更新日期：2011-10-01 00:00:00
abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9114-y
更新日期：2008-06-01 00:00:00
abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF02512996
更新日期：1991-12-01 00:00:00
abstract：:Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9637-8
更新日期：2013-04-01 00:00:00
abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9350-4
更新日期：2010-08-01 00:00:00
abstract：:Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9722-7
更新日期：2014-02-01 00:00:00
abstract：:The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-014-9826-8
更新日期：2014-10-01 00:00:00
abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9825-9
更新日期：2015-02-01 00:00:00
abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9300-1
更新日期：2010-04-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00110-z
更新日期：2019-04-01 00:00:00
abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-5413-8
更新日期：2005-04-01 00:00:00
