Abstract:
:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly in the mammary gland, with 'leakage' of expression in the salivary gland and kidney. The developmental pattern of transgene expression differed from that of the endogenous WAP gene. In particular, recombinant HPC (rHPC) transcripts were detected earlier in pregnancy than WAP RNA, with no significant increase during lactation. This indicates that regulatory elements responsible for developmental regulation are located outside the 4.1 kb mWAP gene promoter fragment, or if present, may be subject to position effects. Precocious expression of the transgene did not compromise the health or nursing abilities of transgenic females. Expression of rHPC affected the appearance of the mammary alveoli and alveolar epithelial cells in lactating transgenic mice. The alveoli were less distended and alveolar epithelial cells appeared cuboidal with centrally positioned nuclei. We suggest that the inefficient intracellular processing of rHPC can alter the histological appearance of alveolar epithelial cells in the transgenic mammary gland.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon Hdoi
10.1007/BF01976765subject
Has Abstractpub_date
1994-11-01 00:00:00pages
335-43issue
6eissn
0962-8819issn
1573-9368journal_volume
3pub_type
杂志文章abstract::Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9091-1
更新日期:2008-04-01 00:00:00
abstract::Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9106-y
更新日期:2008-06-01 00:00:00
abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9563-1
更新日期:2012-06-01 00:00:00
abstract::RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9765-9
更新日期:2014-04-01 00:00:00
abstract::Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombi...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-0389-6
更新日期:2005-06-01 00:00:00
abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00110-z
更新日期:2019-04-01 00:00:00
abstract::Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9978-9
更新日期:2017-02-01 00:00:00
abstract::Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) c...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0087-9
更新日期:2018-10-01 00:00:00
abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02512996
更新日期:1991-12-01 00:00:00
abstract::We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9517-7
更新日期:2012-02-01 00:00:00
abstract::Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9403-8
更新日期:2011-06-01 00:00:00
abstract::Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00141-6
更新日期:2019-08-01 00:00:00
abstract::Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0084-z
更新日期:2018-10-01 00:00:00
abstract::We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02513027
更新日期:1992-03-01 00:00:00
abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title:Transgenic research
pub_type: 杂志文章,meta分析
doi:10.1007/s11248-013-9757-9
更新日期:2014-04-01 00:00:00
abstract::It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/b:trag.0000026072.01351.10
更新日期:2004-04-01 00:00:00
abstract::Standard methods to mark Xenopus laevis individuals like tattooing or clipping toenails are inappropriate for the fast growing and regenerating small froglets and the previously used transponders are too large. In this study we successfully adapted micro transponders to tag these animals. Using these new transponders ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9061-z
更新日期:2007-08-01 00:00:00
abstract::Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9180-9
更新日期:2008-10-01 00:00:00
abstract::Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0083-0
更新日期:2018-10-01 00:00:00
abstract::Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9905-5
更新日期:2015-12-01 00:00:00
abstract::The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9156-1
更新日期:2008-06-01 00:00:00
abstract::The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9685-0
更新日期:2013-08-01 00:00:00
abstract::We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-9587-x
更新日期:2004-10-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9494-x
更新日期:2011-12-01 00:00:00
abstract::Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008886629825
更新日期:1999-04-01 00:00:00
abstract::Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9364-y
更新日期:2010-10-01 00:00:00
abstract::Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9831-y
更新日期:2015-02-01 00:00:00
abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-012-9686-z
更新日期:2013-08-01 00:00:00
abstract::Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To furth...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-9054-y
更新日期:2007-10-01 00:00:00
abstract::Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00