Abstract：
:Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and although the strategies of producing marker-free site-specific integration plants have been discussed, the concept has not been demonstrated. In the present study, we combined two approaches to site-specific recombination and demonstrated the concepts for removing the marker after site-specific integration for producing marker-free site-specific transgenic plants.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Nanto K,Ebinuma Hdoi
10.1007/s11248-007-9106-ysubject
Has Abstractpub_date
2008-06-01 00:00:00pages
337-44issue
3eissn
0962-8819issn
1573-9368journal_volume
17pub_type
杂志文章abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00208-9
更新日期：2020-08-01 00:00:00
abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-007-9083-1
更新日期：2007-06-01 00:00:00
abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9765-9
更新日期：2014-04-01 00:00:00
abstract：:Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0012-7
更新日期：2017-06-01 00:00:00
abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9556-0
更新日期：2012-06-01 00:00:00
abstract：:A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfp marker gene conferring streptomycin-spectinomycin resistance and green fluorescence under UV light. Biolistic transformat...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022110402302
更新日期：2003-02-01 00:00:00
abstract：:We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-2808-5
更新日期：2004-12-01 00:00:00
abstract：:Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-4349-y
更新日期：2005-08-01 00:00:00
abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01979922
更新日期：1996-01-01 00:00:00
abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9219-y
更新日期：2009-02-01 00:00:00
abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9858-0
更新日期：2015-06-01 00:00:00
abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9828-6
更新日期：2015-02-01 00:00:00
abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/BF01976021
更新日期：1994-01-01 00:00:00
abstract：:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9364-y
更新日期：2010-10-01 00:00:00
abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-006-0007-2
更新日期：2006-06-01 00:00:00
abstract：:During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01973758
更新日期：1995-11-01 00:00:00
abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9821-0
更新日期：2015-02-01 00:00:00
abstract：:Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01974093
更新日期：1994-03-01 00:00:00
abstract：:The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and pa...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/b:trag.0000040053.23687.9c
更新日期：2004-08-01 00:00:00
abstract：:Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9085-z
更新日期：2008-02-01 00:00:00
abstract：:We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1022194120518
更新日期：2003-02-01 00:00:00
abstract：:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9759-7
更新日期：2014-04-01 00:00:00
abstract：:Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9418-1
更新日期：2011-04-01 00:00:00
abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00198-8
更新日期：2020-06-01 00:00:00
abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0084-z
更新日期：2018-10-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-0389-6
更新日期：2005-06-01 00:00:00
abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9156-1
更新日期：2008-06-01 00:00:00
abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008915007271
更新日期：1999-06-01 00:00:00
abstract：:Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9585-8
更新日期：2012-10-01 00:00:00
