Abstract：
:Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thaliana, were regenerated after transformation with Agrobacterium tumefaciens. These plants were able to grow, flower and accumulate more rutin in the presence of 200 mmol/l sodium chloride. Moreover, the content of important nutrients in buckwheat was not affected by the high salinity of the soil. These results demonstrated the potential value of these transgenic plants for agriculture use in saline soil.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Chen LH,Zhang B,Xu ZQdoi
10.1007/s11248-007-9085-zsubject
Has Abstractpub_date
2008-02-01 00:00:00pages
121-32issue
1eissn
0962-8819issn
1573-9368journal_volume
17pub_type
杂志文章abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9629-8
更新日期：2013-02-01 00:00:00
abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01969712
更新日期：1996-05-01 00:00:00
abstract：:The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00153-2
更新日期：2019-08-01 00:00:00
abstract：:The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) w...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9884-6
更新日期：2015-08-01 00:00:00
abstract：:Plant secondary metabolism is very important for traits such as flower color, flavor of food, and resistance against pests and diseases. Moreover, it is the source of many fine chemicals such as drugs, dyes, flavors, and fragrances. It is thus of interest to be able to engineer the secondary metabolite production of t...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1023/a:1008966404981
更新日期：2000-01-01 00:00:00
abstract：:Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008886629825
更新日期：1999-04-01 00:00:00
abstract：:SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in ou...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00215-w
更新日期：2020-12-01 00:00:00
abstract：:Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9722-7
更新日期：2014-02-01 00:00:00
abstract：:Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00208-9
更新日期：2020-08-01 00:00:00
abstract：:Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9479-1
更新日期：2011-10-01 00:00:00
abstract：:We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-9587-x
更新日期：2004-10-01 00:00:00
abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-020-00198-8
更新日期：2020-06-01 00:00:00
abstract：:In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0039-9
更新日期：2017-12-01 00:00:00
abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013986627504
更新日期：2002-02-01 00:00:00
abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01979922
更新日期：1996-01-01 00:00:00
abstract：:During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01973758
更新日期：1995-11-01 00:00:00
abstract：:To commemorate Transgenic Animal Research Conference X, this review summarizes the recent progress in developing genetically engineered livestock species as biomedical models. The first of these conferences was held in 1997, which turned out to be a watershed year for the field, with two significant events occurring. ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9928-6
更新日期：2016-06-01 00:00:00
abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9825-9
更新日期：2015-02-01 00:00:00
abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9101-3
更新日期：2008-04-01 00:00:00
abstract：:To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title：Transgenic research
pub_type： 信件
doi：10.1007/s11248-012-9605-3
更新日期：2012-12-01 00:00:00
abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9477-3
更新日期：2011-10-01 00:00:00
abstract：:A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaer...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01969382
更新日期：1993-03-01 00:00:00
abstract：:Genomic DNA blot hybridization is traditionally used to demonstrate that, via genetic transformation, foreign genes are integrated into host genomes. However, in large genome species, such as Allium cepa L., the use of genomic DNA blot hybridization is pushed towards its limits, because a considerable quantity of DNA ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1016633410041
更新日期：2001-06-01 00:00:00
abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9765-9
更新日期：2014-04-01 00:00:00
abstract：:Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approac...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9091-1
更新日期：2008-04-01 00:00:00
abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013007329936
更新日期：2001-12-01 00:00:00
abstract：:Cre/loxP recombination is a powerful strategy widely used for in vivo conditional gene targeting. This technique has made possible many important discoveries of gene function in normal and disease biology. However, due to the transgenic nature of most Cre mouse strains undesired phenotypes occasionally occur in Cre mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9828-6
更新日期：2015-02-01 00:00:00
abstract：:We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-011-9517-7
更新日期：2012-02-01 00:00:00
abstract：:Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9715-6
更新日期：2013-12-01 00:00:00
abstract：:The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-015-9899-z
更新日期：2015-10-01 00:00:00
