Molecular characterization of transgenic shallots (Allium cepa L.) by adaptor ligation PCR (AL-PCR) and sequencing of genomic DNA flanking T-DNA borders.

abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

journal_title：Transgenic research

authors： Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

更新日期：1991-12-01 00:00:00

abstract：:The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...

journal_title：Transgenic research

authors： Kurek I,Stöger E,Dulberger R,Christou P,Breiman A

更新日期：2002-08-01 00:00:00

abstract：:Embryonic stem (ES) cells provide a unique tool for cell-mediated gene transfer and targeted gene mutations due to the possibility of in vitro selection of desired genotypes. When selected cells contribute to the germ line in chimaeric embryos, transgenic animals may be generated with modified genetic traits. Though t...

journal_title：Transgenic research

authors： Béjar J,Hong Y,Alvarez MC

更新日期：2002-06-01 00:00:00

abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

journal_title：Transgenic research

authors： Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

更新日期：2011-06-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

journal_title：Transgenic research

authors： He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

更新日期：2013-08-01 00:00:00

abstract：:The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...

journal_title：Transgenic research

authors： Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WA

更新日期：2002-04-01 00:00:00

abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

journal_title：Transgenic research

authors： O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

更新日期：2002-10-01 00:00:00

abstract：:Transgenic mouse models mediating conditional temporal and spatial regulation of gene expression to the respiratory epithelium were developed utilizing the reverse tetracycline transactivator (rtTA) expressed under the control of SP-C and CCSP promoters. Luciferase activity was detected in the lungs of fetal and adult...

journal_title：Transgenic research

authors： Perl AK,Tichelaar JW,Whitsett JA

更新日期：2002-02-01 00:00:00

abstract：:We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inv...

journal_title：Transgenic research

authors： Di Nicola-Negri E,Brunetti A,Tavazza M,Ilardi V

更新日期：2005-12-01 00:00:00

abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...

journal_title：Transgenic research

authors： Nottle MB,Haskard KA,Verma PJ,Du ZT,Grupen CG,McIlfatrick SM,Ashman RJ,Harrison SJ,Barlow H,Wigley PL,Lyons IG,Cowan PJ,Crawford RJ,Tolstoshev PL,Pearse MJ,Robins AJ,d'Apice AJ

更新日期：2001-12-01 00:00:00

abstract：:The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were mod...

journal_title：Transgenic research

authors： Saini KS,Byrne CR,Leish Z,Pruss CA,Rigby NW,Brownlee AG,Nancarrow CD,Ward KA

更新日期：1996-11-01 00:00:00

abstract：:The definition of a disease is fundamentally difficult, even if one considers only genetically based diseases. In its broadest sense, disease can be defined as any deviation from the norm that results in a physiological disadvantage. Natural selection ensures that the norm for any given species is constantly changing....

journal_title：Transgenic research

authors： Wassenegger M

更新日期：2002-12-01 00:00:00

abstract：:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

journal_title：Transgenic research

authors： Sivparsad BJ,Gubba A

更新日期：2014-04-01 00:00:00

abstract：:A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...

journal_title：Transgenic research

authors： Chakraborty M,Reddy PS,Mustafa G,Rajesh G,Narasu VM,Udayasuriyan V,Rana D

更新日期：2016-10-01 00:00:00

abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...

journal_title：Transgenic research

authors： Uusi-Oukari M,Hyttinen JM,Korhonen VP,Västi A,Alhonen L,Jänne OA,Jänne J

更新日期：1997-01-01 00:00:00

abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

journal_title：Transgenic research

authors： Landel CP,Dunlap J,Patton JB,Manser T

更新日期：2013-02-01 00:00:00

abstract：:With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...

journal_title：Transgenic research

authors： Devos Y,Cougnon M,Vergucht S,Bulcke R,Haesaert G,Steurbaut W,Reheul D

更新日期：2008-12-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

journal_title：Transgenic research

authors： Zbikowska HM

更新日期：2003-08-01 00:00:00

abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

journal_title：Transgenic research

authors： Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

更新日期：2004-04-01 00:00:00

abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...

journal_title：Transgenic research

authors： Goldman D,Hankin M,Li Z,Dai X,Ding J

更新日期：2001-01-01 00:00:00

abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...

journal_title：Transgenic research

authors： Palaudelmàs M,Peñas G,Melé E,Serra J,Salvia J,Pla M,Nadal A,Messeguer J

更新日期：2009-08-01 00:00:00

abstract：:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

journal_title：Transgenic research

authors： Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

更新日期：2003-12-01 00:00:00

abstract：:Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in see...

journal_title：Transgenic research

authors： Ashikawa I,Mori M,Nakamura S,Abe F

更新日期：2014-08-01 00:00:00

abstract：:We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...

journal_title：Transgenic research

authors： Peretz Y,Levy M,Avisar E,Edelbaum O,Rabinowitch H,Sela I

更新日期：2008-08-01 00:00:00

abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

journal_title：Transgenic research

authors： Zhang XD,Letham DS,Zhang R,Higgins TJ

更新日期：1996-01-01 00:00:00

abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

journal_title：Transgenic research

authors： Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

更新日期：2011-12-01 00:00:00

abstract：:The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...

journal_title：Transgenic research

authors： Vervoort VS,Lu M,Valencia F,Lesperance J,Breier G,Oshima R,Pasquale EB

更新日期：2008-06-01 00:00:00

abstract：:We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...

journal_title：Transgenic research

authors： Vain P,Harvey A,Worland B,Ross S,Snape JW,Lonsdale D

更新日期：2004-12-01 00:00:00

abstract：:Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...

journal_title：Transgenic research

authors： Buriani G,Mancini C,Benvenuto E,Baschieri S

更新日期：2011-04-01 00:00:00