Abstract：
:The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although subcellular localization was not proven. SI was found to exist as two forms, one coinciding with authentic BPTI on western blots and the second marginally larger due to retention of the C-terminal peptide. Both were retained on a trypsin-agarose affinity gel and had inhibitory activity. Newly emergent leaves contained predominantly the large form whereas senescent leaves had little except the fully processed form present. Intermediate-aged leaves showed a gradual change indicating that a slow processing of the inhibitor peptide was occurring. The stability of SI was shown by the presence of protein at high levels in completely senescent leaves. Modifications to the cDNA (3' and 5' changes and minor codon changes) resulted in a 20-fold variation in expression. Expression of modified SI in transgenic tobacco leaves at 0.5% total soluble protein reduced both survival and growth of Helicoverpa armigera larvae feeding on leaves from the late first instar. In larvae surviving for 8 days, midgut trypsin activity was reduced in SI-tobacco fed larvae, while chymotrypsin activity was increased. Activities of leucine aminopeptidase and elastase-like chymotrypsin remained unaltered. The use of SI as an insect resistance factor is discussed.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WAdoi
10.1023/a:1015210919077subject
Has Abstractpub_date
2002-04-01 00:00:00pages
161-73issue
2eissn
0962-8819issn
1573-9368journal_volume
11pub_type
杂志文章abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...
journal_title：Transgenic research
pub_type： 杂志文章,meta分析
doi：10.1007/s11248-013-9757-9
更新日期：2014-04-01 00:00:00
abstract：:Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-8293-7
更新日期：2005-10-01 00:00:00
abstract：:The relative proportion of kynurenine aminotransferase (KAT) I-IV activities in the brain is similar between humans and rats. Moreover, KAT II is considered to be the main enzyme for kynurenic acid production in the brain. Taken together, human KAT II knock-in (hKAT II KI) rats will become a valuable tool for the eval...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9909-1
更新日期：2015-12-01 00:00:00
abstract：:Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-009-9288-6
更新日期：2010-02-01 00:00:00
abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-013-9688-5
更新日期：2013-10-01 00:00:00
abstract：:During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be medi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01973758
更新日期：1995-11-01 00:00:00
abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008928121846
更新日期：2000-02-01 00:00:00
abstract：:Plant transformation, via Agrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during fiel...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/BF01974093
更新日期：1994-03-01 00:00:00
abstract：:We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-004-9587-x
更新日期：2004-10-01 00:00:00
abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-005-4350-5
更新日期：2005-10-01 00:00:00
abstract：:One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-016-9932-x
更新日期：2016-06-01 00:00:00
abstract：:Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na(+)/H(+) antiporter gene from Arabidopsis thali...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9085-z
更新日期：2008-02-01 00:00:00
abstract：:Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008886629825
更新日期：1999-04-01 00:00:00
abstract：:The generation is reported of transgenic mice expressing human granulocyte-macrophage colony-stimulating factor (GM-CSF) or human erythropoietin (EPO) under the control of bovine alpha s1-casein regulatory sequences. GM-CSF expression was specific to the mammary gland, and levels of human GM-CSF in transgenic mouse mi...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1018461201385
更新日期：1997-01-01 00:00:00
abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1008915007271
更新日期：1999-06-01 00:00:00
abstract：:Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-015-9905-5
更新日期：2015-12-01 00:00:00
abstract：:We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9146-3
更新日期：2008-08-01 00:00:00
abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-007-9114-y
更新日期：2008-06-01 00:00:00
abstract：:In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-017-0039-9
更新日期：2017-12-01 00:00:00
abstract：:Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-019-00141-6
更新日期：2019-08-01 00:00:00
abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-018-0084-z
更新日期：2018-10-01 00:00:00
abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-010-9377-6
更新日期：2010-12-01 00:00:00
abstract：:Neural precursor cell expressed, developmentally down-regulated 9 (Nedd9, Casl, Hef1, p105cas, Ef1) is a scaffolding protein that assembles complexes involved in regulating cell adhesion, migration, division, and survival. Nedd9 is found very early in the developing embryonic nervous system. A highly conserved complex...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9825-9
更新日期：2015-02-01 00:00:00
abstract：:Transthyretin (TTR) associated amyloidosis is an autosomal dominant disorder characterized by peripheral and autonomic neuropathy. Both genetic and environmental factors are thought to be involved in development of TTR associated amyloidosis. Previously, we demonstrated that amyloid deposition was observed in various ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-008-9180-9
更新日期：2008-10-01 00:00:00
abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/BF01976021
更新日期：1994-01-01 00:00:00
abstract：:Three lines of transgenic tilapia (Oreochromis niloticus) fish were generated with a construct containing a lacZ reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. All these three lines contain different copy numbers of transgenes and the levels of lacZ expression were found to be relate...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1026517212807
更新日期：2000-12-01 00:00:00
abstract：:A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1023/a:1013007329936
更新日期：2001-12-01 00:00:00
abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-014-9831-y
更新日期：2015-02-01 00:00:00
abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...
journal_title：Transgenic research
pub_type： 杂志文章,评审
doi：10.1007/s11248-006-0024-1
更新日期：2006-08-01 00:00:00
abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...
journal_title：Transgenic research
pub_type： 杂志文章
doi：10.1007/s11248-012-9686-z
更新日期：2013-08-01 00:00:00
