Construction of a transgenic pig model overexpressing polycystic kidney disease 2 (PKD2) gene.

abstract：:Wheat and barley are the major temperate cereals, being used for food, feed and industrial raw material. However, in all cases the quality may be limited by the amount, composition and properties of the grain storage proteins. We describe how a combination of biochemical and molecular studies has led to an understandi...

journal_title：Transgenic research

authors： Shewry PR,Tatham AS,Halford NG,Barker JH,Hannappel U,Gallois P,Thomas M,Kreis M

更新日期：1994-01-01 00:00:00

abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

journal_title：Transgenic research

authors： Pai CW,Chen YH

更新日期：2010-08-01 00:00:00

abstract：:The ability to tightly control transgene expression in vivo provides an opportunity to determine the role of certain gene products at different times during development and/or in response to different stimuli. We have characterized and evaluated a tetracycline-responsive endothelial-specific binary system during mouse...

journal_title：Transgenic research

authors： Sarao R,Dumont DJ

更新日期：1998-11-01 00:00:00

abstract：:Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...

journal_title：Transgenic research

authors： Je J,Song C,Hwang JE,Chung WS,Lim CO

更新日期：2014-02-01 00:00:00

abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

journal_title：Transgenic research

authors： Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

更新日期：2018-10-01 00:00:00

abstract：:We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...

journal_title：Transgenic research

authors： Petrie JR,Shrestha P,Belide S,Mansour MP,Liu Q,Horne J,Nichols PD,Singh SP

更新日期：2012-02-01 00:00:00

abstract：:The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells f...

journal_title：Transgenic research

authors： Reed WA,Elzer PH,Enright FM,Jaynes JM,Morrey JD,White KL

更新日期：1997-09-01 00:00:00

abstract：:Cre/LoxP mediated chromosomal engineering in embryonic stem (ES) cells has a variety of applications, including the creation of model systems for studying aneuploidy. Targeted meiotic recombination (TAMERE) was proposed as a high efficiency in vivo alternative to effect Cre-mediated recombination, in which Cre recombi...

journal_title：Transgenic research

authors： Olson LE,Tien J,South S,Reeves RH

更新日期：2005-06-01 00:00:00

abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...

journal_title：Transgenic research

authors： Hungria M,Nakatani AS,Souza RA,Sei FB,de Oliveira Chueire LM,Arias CA

更新日期：2015-02-01 00:00:00

abstract：:Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...

journal_title：Transgenic research

authors： Levin JS,Thompson WF,Csinos AS,Stephenson MG,Weissinger AK

更新日期：2005-04-01 00:00:00

abstract：:Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...

journal_title：Transgenic research

authors： Ferry N,Mulligan EA,Stewart CN,Tabashnik BE,Port GR,Gatehouse AM

更新日期：2006-08-01 00:00:00

abstract：:Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...

journal_title：Transgenic research

authors： Thomas AD,Murray JD,Oberbauer AM

更新日期：2005-08-01 00:00:00

abstract：:Transgenic plants severely suppressed in the activity of cinnamoyl-CoA reductase were produced by introduction of a partial sense CCR transgene into tobacco. Five transgenic lines with CCR activities ranging from 2 to 48% of wild-type values were selected for further study. Some lines showed a range of aberrant phenot...

journal_title：Transgenic research

authors： O'Connell A,Holt K,Piquemal J,Grima-Pettenati J,Boudet A,Pollet B,Lapierre C,Petit-Conil M,Schuch W,Halpin C

更新日期：2002-10-01 00:00:00

abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...

journal_title：Transgenic research

authors： Pareddy D,Chennareddy S,Anthony G,Sardesai N,Mall T,Minnicks T,Karpova O,Clark L,Griffin D,Bishop B,Shumway N,Samuel P,Smith K,Sarria R

更新日期：2020-06-01 00:00:00

abstract：:Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...

journal_title：Transgenic research

authors： Van Eenennaam AL

更新日期：2019-08-01 00:00:00

abstract：:Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

journal_title：Transgenic research

authors： Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

更新日期：2016-08-01 00:00:00

abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...

journal_title：Transgenic research

authors： Khan SA,Zafar Y,Briddon RW,Malik KA,Mukhtar Z

更新日期：2006-06-01 00:00:00

abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

journal_title：Transgenic research

authors： Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

更新日期：2011-10-01 00:00:00

abstract：:The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...

journal_title：Transgenic research

authors： Allegra S,Bouazza L,Benetollo C,Li JY,Langlois D

更新日期：2005-02-01 00:00:00

abstract：:The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...

journal_title：Transgenic research

authors： de Paiva Rolla AA,de Fátima Corrêa Carvalho J,Fuganti-Pagliarini R,Engels C,do Rio A,Marin SR,de Oliveira MC,Beneventi MA,Marcelino-Guimarães FC,Farias JR,Neumaier N,Nakashima K,Yamaguchi-Shinozaki K,Nepomuceno AL

更新日期：2014-02-01 00:00:00

abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...

journal_title：Transgenic research

authors： Ricroch A,Bergé JB,Kuntz M

更新日期：2010-02-01 00:00:00

abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...

journal_title：Transgenic research

authors： Ramessar K,Peremarti A,Gómez-Galera S,Naqvi S,Moralejo M,Muñoz P,Capell T,Christou P

更新日期：2007-06-01 00:00:00

abstract：:Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...

journal_title：Transgenic research

authors： Lin YS,Yang CC,Hsu CC,Hsu JT,Wu SC,Lin CJ,Cheng WT

更新日期：2015-02-01 00:00:00

abstract：:As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...

journal_title：Transgenic research

authors： Chapotin SM,Wolt JD

更新日期：2007-12-01 00:00:00

abstract：:The isopentenyl transferase gene (ipt) from Agrobacterium tumefaciens was isolated and introduced, via a disarmed binary vector, into tobacco using the Agrobacterium tumefaciens-mediated gene transfer system. The expression of the ipt gene was monitored by RNA hybridization, western blotting and cytokinin analysis. Th...

journal_title：Transgenic research

authors： Zhang XD,Letham DS,Zhang R,Higgins TJ

更新日期：1996-01-01 00:00:00

abstract：:Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

journal_title：Transgenic research

authors： Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

更新日期：2005-10-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

journal_title：Transgenic research

authors： Landel CP,Dunlap J,Patton JB,Manser T

更新日期：2013-02-01 00:00:00

abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...

journal_title：Transgenic research

authors： Kawazu Y,Fujiyama R,Noguchi Y,Kubota M,Ito H,Fukuoka H

更新日期：2010-04-01 00:00:00

abstract：:Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...

journal_title：Transgenic research

authors： Tang Y,Bai H,Urs S,Wang Z,Liaw L

更新日期：2013-04-01 00:00:00