Abstract:
:Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to address the problem of multiple virus infections and synergism, this study aimed to develop transgenic sweet potato (cv. Blesbok) plants with broad virus resistance. Coat protein gene segments of SPFMV, SPCSV, SPVG and SPMMV were used to induce gene silencing in transgenic sweet potato. Transformation of apical tips of sweet potato cv. Blesbok was achieved by using Agrobacterium tumefaciens strain LBA4404 harboring the expression cassette. Polymerase chain reaction and Southern blot analyses showed integration of the transgenes occurred in six of the 24 putative transgenic plants and that all plants seemed to correspond to the same transformation event. The six transgenic plants were challenged by graft inoculation with SPFMV, SPCSV, SPVG and SPMMV-infected Ipomoea setosa Ker. Although virus presence was detected using nitrocellulose enzyme-linked immunosorbent assay, all transgenic plants displayed delayed and milder symptoms of chlorosis and mottling of lower leaves when compared to the untransformed control plants. These results warrant further investigation on resistance to virus infection under field conditions.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Sivparsad BJ,Gubba Adoi
10.1007/s11248-013-9759-7subject
Has Abstractpub_date
2014-04-01 00:00:00pages
377-88issue
2eissn
0962-8819issn
1573-9368journal_volume
23pub_type
杂志文章abstract::Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax indust...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0012-7
更新日期:2017-06-01 00:00:00
abstract::We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9146-3
更新日期:2008-08-01 00:00:00
abstract::The silkworm Bombyx mori is a valuable insect that synthesizes bulk amounts of fibroin protein in its posterior silk gland (PSG) and weaves these proteins into silk cocoons. The mechanism by which the fibroin protein is efficiently synthesized and precisely regulated is an important aspect that has yet to be fully elu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00175-w
更新日期:2019-12-01 00:00:00
abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016374128479
更新日期:2002-08-01 00:00:00
abstract::Matrix attachment regions (MARs) are DNA elements that can increase and stabilize transgene expression. We investigated the effect of the RB7 MAR on transgenic virus resistance. Constructs for resistance to tomato spotted wilt virus (TSWV) with and without flanking RB7 MARs were used to transform tobacco and produce h...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-004-5413-8
更新日期:2005-04-01 00:00:00
abstract::Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9114-y
更新日期:2008-06-01 00:00:00
abstract::Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9479-1
更新日期:2011-10-01 00:00:00
abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00110-z
更新日期:2019-04-01 00:00:00
abstract::It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9785-0
更新日期:2014-12-01 00:00:00
abstract::Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9817-9
更新日期:2015-02-01 00:00:00
abstract::The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to ...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-015-9899-z
更新日期:2015-10-01 00:00:00
abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9350-4
更新日期:2010-08-01 00:00:00
abstract::The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-008-9219-y
更新日期:2009-02-01 00:00:00
abstract::Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9117-8
更新日期:2008-06-01 00:00:00
abstract::Transgenic plants producing insecticidal proteins from Bacillus thuringiensis (Bt) can control some major insect pests and reduce reliance on sprayed insecticides. However, large scale adoption of this technology has raised concerns about potential negative effects, including evolution of pest resistance to Bt toxins,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0021-4
更新日期:2006-08-01 00:00:00
abstract::In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0039-9
更新日期:2017-12-01 00:00:00
abstract::While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9101-3
更新日期:2008-04-01 00:00:00
abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9939-3
更新日期:2016-08-01 00:00:00
abstract::The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9461-y
更新日期:2011-08-01 00:00:00
abstract::Two commonly used promoters to ubiquitously express transgenes in zebrafish are the Xenopus laevis elongation factor 1 alpha promoter (XlEef1a1) and the zebrafish histone variant H2A.F/Z (h2afv) promoter. Recently, transgenes utilizing these promoters were shown to be silenced in certain adult tissues, particularly th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9152-5
更新日期:2008-04-01 00:00:00
abstract::Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-010-9377-6
更新日期:2010-12-01 00:00:00
abstract::Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9556-0
更新日期:2012-06-01 00:00:00
abstract::Tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA), an aphid pest, Myzus persicae (Sulz.), and a beneficial predator, the 2-spot ladybird (Adalia bipunctata L.) were investigated. Clonal plants expressing GNA at 0.1-0.2% total soluble ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1022904805028
更新日期:2003-04-01 00:00:00
abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4350-5
更新日期:2005-10-01 00:00:00
abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-020-00208-9
更新日期:2020-08-01 00:00:00
abstract::Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene construct...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008886629825
更新日期:1999-04-01 00:00:00
abstract::A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaer...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF01969382
更新日期:1993-03-01 00:00:00
abstract::We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisel...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02513027
更新日期:1992-03-01 00:00:00
abstract::Plants of strawberry (cultivar Pájaro) were transformed with three defense related genes: ch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacu...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-2543-6
更新日期:2006-02-01 00:00:00
abstract::The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008915007271
更新日期:1999-06-01 00:00:00