Abstract:
:It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted elsewhere. The acceptance of data generated in CFTs "out of country" can only be realized in practice if the agro-climatic zone where a CFT is conducted is demonstrably representative of the agro-climatic zones in those geographies to which the data will be transported. In an attempt to elaborate this idea, a multi-disciplinary Working Group of scientists collaborated to develop a conceptual framework and associated process that can be used by the regulated and regulatory communities to support transportability of CFT data for environmental risk assessment (ERA). As proposed here, application of the conceptual framework provides a scientifically defensible process for evaluating if existing CFT data from remote sites are relevant and/or sufficient for local ERAs. Additionally, it promotes a strategic approach to identifying CFT site locations so that field data will be transportable from one regulatory jurisdiction to another. Application of the framework and process should be particularly beneficial to public sector product developers and small enterprises that develop innovative GE events but cannot afford to replicate redundant CFTs, and to regulatory authorities seeking to improve the deployment of limited institutional resources.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MAdoi
10.1007/s11248-014-9785-0subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1025-41issue
6eissn
0962-8819issn
1573-9368journal_volume
23pub_type
杂志文章abstract::In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-011-9563-1
更新日期:2012-06-01 00:00:00
abstract::The genes that regulate the formation of blood vessels in adult tissues represent promising therapeutic targets because angiogenesis plays a role in many diseases, including cancer. We wished to develop a mouse model allowing characterization of gene function in adult angiogenic vasculature while minimizing effects on...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9156-1
更新日期:2008-06-01 00:00:00
abstract::To examine the consequences of increased apolipoprotein A-I production on cholesterol and lipoprotein metabolism, we have produced two lines of transgenic rats; one expressing moderate and one very high levels of human apolipoprotein A-I. The rats were produced by microinjection of a 13 kbp DNA fragment containing the...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/BF02528779
更新日期:1992-05-01 00:00:00
abstract::We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-013-9711-x
更新日期:2013-12-01 00:00:00
abstract::Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-014-9831-y
更新日期:2015-02-01 00:00:00
abstract::While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9101-3
更新日期:2008-04-01 00:00:00
abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9147-2
更新日期:2008-08-01 00:00:00
abstract::Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9978-9
更新日期:2017-02-01 00:00:00
abstract::The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat pl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1016374128479
更新日期:2002-08-01 00:00:00
abstract::Temporal control of site-specific recombination is commonly achieved by using a tamoxifen-inducible form of Cre or Flp recombinases. Although powerful protocols of induction have been developed for gene inactivation at adult stages or during embryonic development, induction of recombination at late gestational or earl...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9905-5
更新日期:2015-12-01 00:00:00
abstract::Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9879-3
更新日期:2015-08-01 00:00:00
abstract::A chimeric Bacillus thuringiensis toxin (Bt) gene, cry2AX1was cloned in a bi-selectable marker free binary vector construct. The cry2AX1 gene, driven by the Chrysanthemum rbcS1 promoter, was introduced into JK1044R, the restorer line (Oryza sativa L. ssp. Indica) of a notified commercially grown rice hybrid in India, ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-016-9954-4
更新日期:2016-10-01 00:00:00
abstract::Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00141-6
更新日期:2019-08-01 00:00:00
abstract::The silk gland of silkworm Bombyx mori, is one of the most important organs that has been fully studied and utilized so far. It contributes finest silk fibers to humankind. The silk gland has excellent ability of synthesizing silk proteins and is a kind tool to produce some useful recombinant proteins, which can be wi...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-014-9826-8
更新日期:2014-10-01 00:00:00
abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9350-4
更新日期:2010-08-01 00:00:00
abstract::The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00153-2
更新日期:2019-08-01 00:00:00
abstract::Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-009-9288-6
更新日期:2010-02-01 00:00:00
abstract::Xylan is one of the main anti-nutritional factors in pig's feed. Although supplementation of β-xylanase in diet can improve the utilization of nutrients in animals, it is limited by feed cost, manufacturing process and storage stability. To determine whether the expression of endogenous β-xylanase gene xynB in vivo ca...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-019-00110-z
更新日期:2019-04-01 00:00:00
abstract::Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used beta-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, o...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9107-x
更新日期:2007-12-01 00:00:00
abstract::As the United States moves toward a plant-based bioeconomy, a large research and development effort is focused on creating new feedstocks to meet biomass demand for biofuels, bioenergy, and specialized bioproducts, such as industrial compounds and biomaterial precursors. Most bioeconomy projections assume the widespre...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-007-9122-y
更新日期:2007-12-01 00:00:00
abstract::Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-006-0007-2
更新日期:2006-06-01 00:00:00
abstract::We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-007-9146-3
更新日期:2008-08-01 00:00:00
abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1023/a:1024267416522
更新日期:2003-08-01 00:00:00
abstract::The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-017-0054-x
更新日期:2018-02-01 00:00:00
abstract::Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) c...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-018-0087-9
更新日期:2018-10-01 00:00:00
abstract::In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1012244404020
更新日期:2001-10-01 00:00:00
abstract::Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-005-4349-y
更新日期:2005-08-01 00:00:00
abstract::To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...
journal_title:Transgenic research
pub_type: 信件
doi:10.1007/s11248-012-9605-3
更新日期:2012-12-01 00:00:00
abstract::Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...
journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1007/s11248-015-9876-6
更新日期:2015-10-01 00:00:00
abstract::One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...
journal_title:Transgenic research
pub_type: 杂志文章,评审
doi:10.1007/s11248-016-9932-x
更新日期:2016-06-01 00:00:00