Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00

abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

journal_title：Transgenic research

authors： Landel CP,Dunlap J,Patton JB,Manser T

更新日期：2013-02-01 00:00:00

abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...

journal_title：Transgenic research

authors： Quijano CD,Brunner S,Keller B,Gruissem W,Sautter C

更新日期：2015-02-01 00:00:00

abstract：:Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used th...

journal_title：Transgenic research

authors： Naim F,Dugdale B,Kleidon J,Brinin A,Shand K,Waterhouse P,Dale J

更新日期：2018-10-01 00:00:00

abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

journal_title：Transgenic research

authors： Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

更新日期：2014-04-01 00:00:00

abstract：:The bacterial gene chloramphenicol acetyltransferase (CAT) is a widely used reporter in both in-vitro and in-vivo studies of genetic regulation. We have recently generated novel rat transgenic lines carrying an arylalkylamine N-acetyltransferase (AA-NAT) promoter-reporter construct in which CAT (with associated SV40 s...

journal_title：Transgenic research

authors： Burke ZD,Wells T,Carter DA,Baler R

更新日期：2000-02-01 00:00:00

abstract：:Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blo...

journal_title：Transgenic research

authors： Yao J,Pang Y,Qi H,Wan B,Zhao X,Kong W,Sun X,Tang K

更新日期：2003-12-01 00:00:00

abstract：:In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture)...

journal_title：Transgenic research

authors： van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BF

更新日期：2001-10-01 00:00:00

abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

journal_title：Transgenic research

authors： Sakamoto T

更新日期：2006-08-01 00:00:00

abstract：:The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...

journal_title：Transgenic research

authors： Cunha NB,Murad AM,Ramos GL,Maranhão AQ,Brígido MM,Araújo AC,Lacorte C,Aragão FJ,Covas DT,Fontes AM,Souza GH,Vianna GR,Rech EL

更新日期：2011-08-01 00:00:00

abstract：:Although genetic transformation of soybean dates back to over two decades, the process remains inefficient. Here, we report the development of an organogenesis-based transformation method of soybean that resulted in an average transformation frequency of 18.7%. This improved method resorts to Agrobacterium-mediated tr...

journal_title：Transgenic research

authors： Pareddy D,Chennareddy S,Anthony G,Sardesai N,Mall T,Minnicks T,Karpova O,Clark L,Griffin D,Bishop B,Shumway N,Samuel P,Smith K,Sarria R

更新日期：2020-06-01 00:00:00

abstract：:SULT2A8 is a male-predominant and liver-specific mouse cytosolic sulfotransferase (SULT) that sulfonates 7α-hydroxyl (7α-OH) bile acids in vitro. Sulfonation regulates bile acid homeostasis, which in turn regulates cholesterol and energy metabolism. Using the Sult2a8-heterozygous (HT) mouse model created earlier in ou...

journal_title：Transgenic research

authors： Wang K,Chan MY,Xu J,Li PS,Liu X,Lee AY,Lee SS,Cheung WT

更新日期：2020-12-01 00:00:00

abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

journal_title：Transgenic research

authors： Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

更新日期：2011-12-01 00:00:00

abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

journal_title：Transgenic research

authors： Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

更新日期：1994-11-01 00:00:00

abstract：:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...

journal_title：Transgenic research

authors： Pavli OI,Panopoulos NJ,Goldbach R,Skaracis GN

更新日期：2010-10-01 00:00:00

abstract：:This study aims to investigate the relative plant growth and reproduction of insect-resistant and susceptible plants following the introgression of an insect-resistance Bt-transgene from Brassica napus, oilseed rape, to wild Brassica juncea. The second backcrossed generation (BC2) from a single backcross family was gr...

journal_title：Transgenic research

authors： Liu YB,Darmency H,Stewart CN Jr,Wei W,Tang ZX,Ma KP

更新日期：2015-06-01 00:00:00

abstract：:While genetically modified upland cotton (Gossypium hirsutum L.) varieties are ranked among the most successful genetically modified organisms (GMO), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. In this study,...

journal_title：Transgenic research

authors： Zhang J,Cai L,Cheng J,Mao H,Fan X,Meng Z,Chan KM,Zhang H,Qi J,Ji L,Hong Y

更新日期：2008-04-01 00:00:00

abstract：:RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...

journal_title：Transgenic research

authors： Xu L,Duan X,Lv Y,Zhang X,Nie Z,Xie C,Ni Z,Liang R

更新日期：2014-04-01 00:00:00

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

journal_title：Transgenic research

authors： Hisatsune C,Ogawa N,Mikoshiba K

更新日期：2013-12-01 00:00:00

abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

journal_title：Transgenic research

authors： Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

更新日期：2011-12-01 00:00:00

abstract：:Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show tha...

journal_title：Transgenic research

authors： Khan SA,Zafar Y,Briddon RW,Malik KA,Mukhtar Z

更新日期：2006-06-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...

journal_title：Transgenic research

authors： Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WA

更新日期：2002-04-01 00:00:00

abstract：:Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the control...

journal_title：Transgenic research

authors： Farré G,Perez-Fons L,Decourcelle M,Breitenbach J,Hem S,Zhu C,Capell T,Christou P,Fraser PD,Sandmann G

更新日期：2016-08-01 00:00:00

abstract：:A green fluorescent protein (GFP) cDNA flanked by inverted terminal repeats (ITR) of adeno-associated virus was constructed. The construct sharply improved the efficiency and specificity of the transient expression of genes driven by two general promoters (cytomegalovirus and medaka beta-actin) and one muscle-specific...

journal_title：Transgenic research

authors： Chou CY,Horng LS,Tsai HJ

更新日期：2001-08-01 00:00:00

abstract：:Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig ...

journal_title：Transgenic research

authors： Lin YS,Yang CC,Hsu CC,Hsu JT,Wu SC,Lin CJ,Cheng WT

更新日期：2015-02-01 00:00:00

abstract：:We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promot...

journal_title：Transgenic research

authors： Mankin SL,Allen GC,Phelan T,Spiker S,Thompson WF

更新日期：2003-02-01 00:00:00

abstract：:Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...

journal_title：Transgenic research

authors： Palaudelmàs M,Peñas G,Melé E,Serra J,Salvia J,Pla M,Nadal A,Messeguer J

更新日期：2009-08-01 00:00:00