Abstract:
:In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture) and one control group, were compared. Development of behaviour, morphological characteristics and body weight of the progeny were tested daily from birth to weaning (0-3 weeks) for all groups. No significant differences in behaviour or morphological development were observed. However, the occurrence of increased (perinatal) pup mortality and increased body weight in the procedural groups, indicates that during the production of gene targeted mice, some of the normal physiological and/or developmental processes can be affected. Therefore, gene targeting procedures should always be accompanied by careful monitoring of health and welfare of the resulting offspring.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
van der Meer M,Baumans V,Hofhuis FM,Olivier B,van Zutphen BFdoi
10.1023/a:1012244404020subject
Has Abstractpub_date
2001-10-01 00:00:00pages
399-408issue
5eissn
0962-8819issn
1573-9368journal_volume
10pub_type
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