Genetically modified crops for the bioeconomy: meeting public and regulatory expectations.

abstract：:Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid chang...

journal_title：Transgenic research

authors： Heckart DL,Schwartz BM,Raymer PL,Parrott WA

更新日期：2016-08-01 00:00:00

abstract：:The roles of transforming growth factor-beta (TGFbeta) in heart or skeletal muscle development and physiology are still the subject of controversies. Our aim was to block, in transgenic mice, the TGFbeta signalling pathway by a dominant negative mutant of the TGFbeta type II receptor fused to the enhanced green fluore...

journal_title：Transgenic research

authors： Allegra S,Bouazza L,Benetollo C,Li JY,Langlois D

更新日期：2005-02-01 00:00:00

abstract：:T-DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates (...

journal_title：Transgenic research

authors： Ahad A,Wolf J,Nick P

更新日期：2003-10-01 00:00:00

abstract：:We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, ...

journal_title：Transgenic research

authors： Vain P,Harvey A,Worland B,Ross S,Snape JW,Lonsdale D

更新日期：2004-12-01 00:00:00

abstract：:A 4.1 kb mouse whey acidic protein (mWAP) promoter was cloned from a C57BL/6 cosmid library. The tissue-specific and developmental pattern of expression of a hybrid gene comprised of the mWAP promoter fragment and the human protein C (HPC) gene was analysed in transgenic mice. The corresponding RNA was detected mainly...

journal_title：Transgenic research

authors： Paleyanda RK,Zhang DW,Hennighausen L,McKnight RA,Lubon H

更新日期：1994-11-01 00:00:00

abstract：:It is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express E...

journal_title：Transgenic research

authors： Lo AC,Fung MK,Au CL,Chan TS,Sauer B,Chung SS,Chung SK

更新日期：2004-04-01 00:00:00

abstract：:The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and diffe...

journal_title：Transgenic research

authors： Hirabayashi M,Yoshizawa Y,Kato M,Tsuchiya T,Nagao S,Hochi S

更新日期：2009-02-01 00:00:00

abstract：:Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich a...

journal_title：Transgenic research

authors： Zeng F,Dong R,Zhao C,Liu D,Zheng E,Song C,Wu Z,Li Z

更新日期：2018-10-01 00:00:00

abstract：:Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the h...

journal_title：Transgenic research

authors： Melton DW,Ketchen AM,Kind AJ,McEwan C,Paisley D,Selfridge J

更新日期：2001-04-01 00:00:00

abstract：:To feed an estimated world population of 8.9 billion by 2050, strategies for increasing grain production must be developed. Several agronomically important traits for increasing yield, such as plant height, grain number, and leaf erectness, have recently been characterized in rice (Oryza sativa L.). These traits are r...

journal_title：Transgenic research

authors： Sakamoto T

更新日期：2006-08-01 00:00:00

abstract：:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering off...

journal_title：Transgenic research

authors： Li ZT,Hopkins DL,Gray DJ

更新日期：2015-10-01 00:00:00

abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

journal_title：Transgenic research

authors： Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

更新日期：2010-06-01 00:00:00

abstract：:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under co...

journal_title：Transgenic research

authors： Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

更新日期：2003-06-01 00:00:00

abstract：:Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

journal_title：Transgenic research

authors： Zhang D,Corlet A,Fouilloux S

更新日期：2008-06-01 00:00:00

abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

journal_title：Transgenic research

authors： Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

更新日期：2010-08-01 00:00:00

abstract：:The disease model of familial amyloidotic polyneuropathy-7.2-hMet30 mice-manifests amyloid deposition that consists of a human amyloidogenic mutant transthyretin (TTR) (TTR V30M). Our previous study found amyloid deposits in 14 of 27 7.2-hMet30 mice at 21-24 months of age. In addition, non-fibrillar TTR deposits were ...

journal_title：Transgenic research

authors： Ohta M,Sugano A,Hatano N,Sato H,Shimada H,Niwa H,Sakaeda T,Tei H,Sakaki Y,Yamamura KI,Takaoka Y

更新日期：2018-02-01 00:00:00

abstract：:Studies on the effects of transgenes in soybean [Glycine max (L.) Merr.] and the associated use of specific herbicides on biological nitrogen fixation (BNF) are still few, although it is important to ensure minimal impacts on benefits provided by the root-nodule symbiosis. Cultivance CV127 transgenic soybean is a cult...

journal_title：Transgenic research

authors： Hungria M,Nakatani AS,Souza RA,Sei FB,de Oliveira Chueire LM,Arias CA

更新日期：2015-02-01 00:00:00

abstract：:Short directly-repeated (DR) DNA enhancer elements of plant viral origin were analyzed for their ability, both individually and in combination, to influence in vivo transcription when inserted upstream from a minimal CaMV35S promoter. Synthetic promoters containing multiple copies and/or combinations of DR cassettes w...

journal_title：Transgenic research

authors： Cazzonelli CI,Velten J

更新日期：2008-06-01 00:00:00

abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

journal_title：Transgenic research

authors： Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

更新日期：2011-06-01 00:00:00

abstract：:Milk and meat from cattle and buffaloes contribute 45% of the global animal protein supply, followed by chickens (31%), and pigs (20%). In 2016, the global cattle population of 1.0 billion head produced 6.5 billion tons of cows' milk, and 66 million tons of beef. In the past century, cattle breeding programs have grea...

journal_title：Transgenic research

authors： Van Eenennaam AL

更新日期：2019-08-01 00:00:00

abstract：:The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

journal_title：Transgenic research

authors： Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

更新日期：1991-12-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

journal_title：Transgenic research

authors： He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

更新日期：2013-08-01 00:00:00

abstract：:Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile s...

journal_title：Transgenic research

authors： Quijano CD,Brunner S,Keller B,Gruissem W,Sautter C

更新日期：2015-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene has recently been confirmed by in vitro tests to possess antifungal abilities. In this study, the CTS1-2 gene has been evaluated for its in planta antifungal activity by constitutive overexpression in tobacco plants to assess its potential to increase ...

journal_title：Transgenic research

authors： Carstens M,Vivier MA,Pretorius IS

更新日期：2003-08-01 00:00:00

abstract：:The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and tr...

journal_title：Transgenic research

authors： Jan FJ,Pang SZ,Fagoaga C,Gonsalves D

更新日期：1999-06-01 00:00:00

abstract：:Alpha1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, alpha1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires ...

journal_title：Transgenic research

authors： Goldman D,Hankin M,Li Z,Dai X,Ding J

更新日期：2001-01-01 00:00:00

abstract：:Selectable marker gene systems are vital for the development of transgenic crops. Since the creation of the first transgenic plants in the early 1980s and their subsequent commercialization worldwide over almost an entire decade, antibiotic and herbicide resistance selectable marker gene systems have been an integral ...

journal_title：Transgenic research

authors： Ramessar K,Peremarti A,Gómez-Galera S,Naqvi S,Moralejo M,Muñoz P,Capell T,Christou P

更新日期：2007-06-01 00:00:00

abstract：:A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiqui...

journal_title：Transgenic research

authors： Christensen AH,Quail PH

更新日期：1996-05-01 00:00:00

abstract：:With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...

journal_title：Transgenic research

authors： Devos Y,Cougnon M,Vergucht S,Bulcke R,Haesaert G,Steurbaut W,Reheul D

更新日期：2008-12-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00