Tissue specific high level expression of a full length human endogenous retrovirus genome transgene, HERV-R, under control of its own promoter in rats.

Abstract:

:Human endogenous retrovirus-R (HERV-R) is one of a full length HERV with a long open reading frame in the env region. The env transcripts are expressed in various human tissues. To investigate the biological role of HERV-R in vivo, we established two lines of transgenic rats carrying a full sequence of HERV-R under control of its own long terminal repeat (LTR) promoter. One line with tandem integration of multiple copies of the transgene expressed HERV-R mRNA in various organs with different expression levels and relatively higher in Harderian and submandibular salivary glands. In another line, the transgene was integrated as a single copy in a haploid and the expression was detected only in Harderian and submandibular salivary glands. In the placenta, one of the tissues with high levels of the HERV-R expression in humans, the transcription was evident starting the 12th day after gestation. A rabbit antiserum against synthetic peptides corresponding with the HERV-R env gene sequence led to detection of an 85 kDa product as a glycoprotein in the Harderian glands. While no pathological significance was observed in either line, the transgenic rat may prove to be a suitable model for analyzing the role of HERV-R function in vivo.

journal_name

Transgenic Res

journal_title

Transgenic research

authors

Tanaka S,Ikeda H,Otsuka N,Yamamoto Y,Sugaya T,Yoshiki T

doi

10.1023/a:1023381819572

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

319-28

issue

3

eissn

0962-8819

issn

1573-9368

journal_volume

12

pub_type

杂志文章
  • Identifying and genotyping transgene integration loci.

    abstract::The random germline integration of genetically engineered transgenes has been a powerful technique to study the role of particular genes in variety of biological processes. Although the identification of the transgene insertion site is often not essential for functional analysis of the transgene, identifying the site ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-008-9190-7

    authors: Liang Z,Breman AM,Grimes BR,Rosen ED

    更新日期:2008-10-01 00:00:00

  • Canadian regulatory aspects of gene editing technologies.

    abstract::The development of gene editing techniques, capable of producing plants and animals with new and improved traits, is revolutionizing the world of plant and animal breeding and rapidly advancing to commercial reality. However, from a regulatory standpoint the Government of Canada views gene editing as another tool that...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-019-00153-2

    authors: Ellens KW,Levac D,Pearson C,Savoie A,Strand N,Louter J,Tibelius C

    更新日期:2019-08-01 00:00:00

  • A study on the influence of different promoter and 5'UTR (URM) cassettes from Arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.

    abstract::Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

    journal_title:Transgenic research

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11248-013-9757-9

    authors: Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

    更新日期:2014-04-01 00:00:00

  • Alteration of floral organ identity by over-expression of IbMADS3-1 in tobacco.

    abstract::The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9420-7

    authors: Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

    更新日期:2011-04-01 00:00:00

  • Silencing of an aphid carboxylesterase gene by use of plant-mediated RNAi impairs Sitobion avenae tolerance of Phoxim insecticides.

    abstract::RNA interference (RNAi) describes the ability of double-stranded RNA (dsRNA) to inhibit homologous gene expression at the RNA level. Its specificity is sequence-based and depends on the sequence of one strand of the dsRNA corresponding to part or all of a specific gene transcript. In this study we adopted plant-mediat...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-013-9765-9

    authors: Xu L,Duan X,Lv Y,Zhang X,Nie Z,Xie C,Ni Z,Liang R

    更新日期:2014-04-01 00:00:00

  • Accumulation of functional recombinant human coagulation factor IX in transgenic soybean seeds.

    abstract::The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9461-y

    authors: Cunha NB,Murad AM,Ramos GL,Maranhão AQ,Brígido MM,Araújo AC,Lacorte C,Aragão FJ,Covas DT,Fontes AM,Souza GH,Vianna GR,Rech EL

    更新日期:2011-08-01 00:00:00

  • Effect of DNA concentration on transgenesis rates in mice and pigs.

    abstract::A retrospective analysis of transgenesis rates obtained in seven pronuclear microinjection programs was undertaken to determine if a relationship existed between the amount of DNA injected and transgenesis rates in the pig. Logistic regression analysis showed that as the concentration of DNA injected increased from 1 ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/a:1013007329936

    authors: Nottle MB,Haskard KA,Verma PJ,Du ZT,Grupen CG,McIlfatrick SM,Ashman RJ,Harrison SJ,Barlow H,Wigley PL,Lyons IG,Cowan PJ,Crawford RJ,Tolstoshev PL,Pearse MJ,Robins AJ,d'Apice AJ

    更新日期:2001-12-01 00:00:00

  • Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.

    abstract::Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus as...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-007-9147-2

    authors: Ling KS,Zhu HY,Gonsalves D

    更新日期:2008-08-01 00:00:00

  • The expression of a mammalian proteinase inhibitor, bovine spleen trypsin inhibitor in tobacco and its effects on Helicoverpa armigera larvae.

    abstract::The cDNA for bovine spleen trypsin inhibitor (SI), a homologue of bovine pancreatic trypsin inhibitor (BPTI), including the natural mammalian presequence was expressed in tobacco using Agrobacterium tumefaciens-mediated transformation. Stable expression required the N-terminal targeting signal presequence although sub...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1023/a:1015210919077

    authors: Christeller JT,Burgess EP,Mett V,Gatehouse HS,Markwick NP,Murray C,Malone LA,Wright MA,Philip BA,Watt D,Gatehouse LN,Lövei GL,Shannon AL,Phung MM,Watson LM,Laing WA

    更新日期:2002-04-01 00:00:00

  • Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis.

    abstract::Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-009-9288-6

    authors: Kvell K,Czömpöly T,Hiripi L,Balogh P,Kóbor J,Bodrogi L,Pongrácz JE,Ritchie WA,Bosze Z

    更新日期:2010-02-01 00:00:00

  • Transportability of confined field trial data for environmental risk assessment of genetically engineered plants: a conceptual framework.

    abstract::It is commonly held that confined field trials (CFTs) used to evaluate the potential adverse environmental impacts of a genetically engineered (GE) plant should be conducted in each country where cultivation is intended, even when relevant and potentially sufficient data are already available from studies conducted el...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-014-9785-0

    authors: Garcia-Alonso M,Hendley P,Bigler F,Mayeregger E,Parker R,Rubinstein C,Satorre E,Solari F,McLean MA

    更新日期:2014-12-01 00:00:00

  • Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis.

    abstract::This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-009-9350-4

    authors: Pai CW,Chen YH

    更新日期:2010-08-01 00:00:00

  • Fish can be first--advances in fish transgenesis for commercial applications.

    abstract::Over the past 15 years researchers have generated stable lines of several species of transgenic fish important for aquaculture. 'All-fish' growth hormone (GH) gene constructs and antifreeze protein (AFP) genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审

    doi:10.1023/a:1024267416522

    authors: Zbikowska HM

    更新日期:2003-08-01 00:00:00

  • Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors.

    abstract::Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-010-9388-3

    authors: Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

    更新日期:2011-02-01 00:00:00

  • Human bcr-abl gene has a lethal effect on embryogenesis.

    abstract::The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/BF02512996

    authors: Heisterkamp N,Jenster G,Kioussis D,Pattengale PK,Groffen J

    更新日期:1991-12-01 00:00:00

  • Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori.

    abstract::To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-009-9328-2

    authors: Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

    更新日期:2010-06-01 00:00:00

  • Biosafety of E. coli beta-glucuronidase (GUS) in plants.

    abstract::The beta-glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicologic...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审

    doi:10.1023/a:1008832711805

    authors: Gilissen LJ,Metz PL,Stiekema WJ,Nap JP

    更新日期:1998-05-01 00:00:00

  • Environmental impact of herbicide regimes used with genetically modified herbicide-resistant maize.

    abstract::With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-envir...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-008-9181-8

    authors: Devos Y,Cougnon M,Vergucht S,Bulcke R,Haesaert G,Steurbaut W,Reheul D

    更新日期:2008-12-01 00:00:00

  • A germline-competent embryonic stem cell line from NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ (NSG) mice.

    abstract::The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-012-9629-8

    authors: Landel CP,Dunlap J,Patton JB,Manser T

    更新日期:2013-02-01 00:00:00

  • Partial correction of abnormal cardiac development in caspase-8-deficient mice by cardiomyocyte expression of p 35.

    abstract::Baculovirus p 35 protein protects cells from apoptotic cell death by inhibiting caspase activation. We have established transgenic mouse lines specifically expressing p 35 in cardiomyocytes, and primary cardiomyocytes isolated from these mice exhibit resistance to staurosporine-induced apoptosis. In a previous study, ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-005-4350-5

    authors: Yajima N,Yamada S,Morisaki T,Toyokuni S,Yonehara S,Sakamaki K

    更新日期:2005-10-01 00:00:00

  • Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant.

    abstract::Seedling stand establishment is a critical factor affecting crop yield in low-precipitation agricultural regions. This is especially true for small seeded crops, such as Camelina (Camelina sativa) and canola (Brassica napus), that need to be planted shallow. Deeper planting would be desirable so that seeds can access ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-020-00208-9

    authors: Sharma Koirala P,Neff MM

    更新日期:2020-08-01 00:00:00

  • Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications.

    abstract::Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using re...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-007-9114-y

    authors: Zhang D,Corlet A,Fouilloux S

    更新日期:2008-06-01 00:00:00

  • Development of transgenic sweet potato with multiple virus resistance in South Africa (SA).

    abstract::Multiple infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KwaZulu-Natal, South Africa. In order to addres...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-013-9759-7

    authors: Sivparsad BJ,Gubba A

    更新日期:2014-04-01 00:00:00

  • Protection against vascular leak in neprilysin transgenic mice with complex overexpression pattern.

    abstract::Neprilysin (NEP) is a cell surface metallopeptidase found in many tissues. Based mostly on pharmacological manipulations, NEP has been thought to protect blood vessels from plasma extravasation. We have suggested that NEP may protect against pulmonary vascular injury. However, these prior studies did not utilize mice ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-016-9969-x

    authors: Wick MJ,Loomis ZL,Harral JW,Le M,Wehling CA,Miller YE,Dempsey EC

    更新日期:2016-12-01 00:00:00

  • Transgene transmission to progeny by oMt1a-oGH transgenic mice.

    abstract::Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation ...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-005-4349-y

    authors: Thomas AD,Murray JD,Oberbauer AM

    更新日期:2005-08-01 00:00:00

  • Gene targeting, genome editing: from Dolly to editors.

    abstract::One of the most powerful strategies to investigate biology we have as scientists, is the ability to transfer genetic material in a controlled and deliberate manner between organisms. When applied to livestock, applications worthy of commercial venture can be devised. Although initial methods used to generate transgeni...

    journal_title:Transgenic research

    pub_type: 杂志文章,评审

    doi:10.1007/s11248-016-9932-x

    authors: Tan W,Proudfoot C,Lillico SG,Whitelaw CB

    更新日期:2016-06-01 00:00:00

  • Effect of volunteers on maize gene flow.

    abstract::Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate a...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-009-9250-7

    authors: Palaudelmàs M,Peñas G,Melé E,Serra J,Salvia J,Pla M,Nadal A,Messeguer J

    更新日期:2009-08-01 00:00:00

  • A T7-driven silencing system in transgenic plants expressing T7 RNA polymerase is a nuclear process.

    abstract::We previously demonstrated a case of silencing in transgenic plants expressing T7 RNA polymerase in which expression of a reporter gene placed under the control of the T7 promoter was silenced. Here we demonstrate that endogenous genes can be silenced by the same system. The T7-driven silencing system does not conform...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-007-9146-3

    authors: Peretz Y,Levy M,Avisar E,Edelbaum O,Rabinowitch H,Sela I

    更新日期:2008-08-01 00:00:00

  • Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT).

    abstract::Childhood-onset dermatitis is one of the most common skin disorders in children. Although various mouse models that mirror aspects of dermatitis have become available, there is still a need for an animal model that develops dermatitis in childhood and is more suitable for performing tissue transplantation experiments....

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-016-9939-3

    authors: Kaneko R,Sato A,Hamada S,Yagi T,Ohsawa I,Ohtsuki M,Kobayashi E,Hirabayashi M,Murakami T

    更新日期:2016-08-01 00:00:00

  • Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits.

    abstract::Vitis vinifera L. cv. 'Thompson Seedless' presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that partici...

    journal_title:Transgenic research

    pub_type: 杂志文章

    doi:10.1007/s11248-006-9044-0

    authors: Hanania U,Velcheva M,Or E,Flaishman M,Sahar N,Perl A

    更新日期:2007-08-01 00:00:00