Abstract:
:Pierce's disease (PD) caused by Xylella fastidiosa prevents cultivation of grapevine (Vitis vinifera) and susceptible hybrids in the southeastern United States and poses a major threat to the grape industry of California and Texas. Genetic resistance is the only proven control of X. fastidiosa. Genetic engineering offers an alternative to heretofore ineffective conventional breeding in order to transfer only PD resistance traits into elite cultivars. A synthetic gene encoding lytic peptide LIMA-A was introduced into V. vinifera and a Vitis hybrid to assess in planta inhibition of X. fastidiosa. Over 1050 independent transgenic plant lines were evaluated in the greenhouse, among which nine lines were selected and tested under naturally-inoculated field conditions. These selected plant lines in the greenhouse remain disease-free for 10 years, to date, even with multiple manual pathogen inoculations. However, all these lines in the field, including a grafted transgenic rootstock, succumbed to PD within 7 years. We conclude that in planta production of antimicrobial lytic peptides does not provide durable PD resistance to grapevine under field conditions.
journal_name
Transgenic Resjournal_title
Transgenic researchauthors
Li ZT,Hopkins DL,Gray DJdoi
10.1007/s11248-015-9876-6subject
Has Abstractpub_date
2015-10-01 00:00:00pages
821-36issue
5eissn
0962-8819issn
1573-9368pii
10.1007/s11248-015-9876-6journal_volume
24pub_type
杂志文章abstract::At least 25 wild type and high peroxidase tobacco Nicotiana tabacum L. plants were examined semiweekly over several weeks for pest insect distribution and damage in a 2 year field study. Incidence and/or severity of naturally occurring caterpillar damage (dingy cutworm (Feltia ducens Walker), black cutworm (Agrotis ip...
journal_title:Transgenic research
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journal_title:Transgenic research
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Transgenic research
pub_type: 杂志文章
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journal_title:Transgenic research
pub_type: 杂志文章
doi:10.1023/a:1008838616203
更新日期:1998-07-01 00:00:00
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pub_type: 杂志文章,评审
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